方法采用聚合酶链反应技术（PCR）检测沙眼衣原体和解脲支原体的DNA。
Methods DNA of CT and UU was detected with polymerase chain reaction(PCR) technique.
方法以一段特异性通用引物并配合热启动聚合酶链反应技术来检测临床标本中的棘阿米巴原虫。
Methods We used a hot initiated PCR based method with asset of universal acanthamoeba specific primers to detect acanthamoeba.
方法：应用半巢式聚合酶链反应技术检测113例孕产妇血清及其新生儿脐血的TTV—DNA。
Methods: TTV-DNA of 113 cases of parturients' blood serum and neonatal umbilical blood were detected by semi-nested polymerase chain reaction.
目的采用顺序特异引物聚合酶链反应技术(PCRSSP)，建立汉族人群HLAB40交叉反应组高分辨度dna分型方法。
Objective To establish a method of high resolution DNA typing for HLA B40 cross reactive groups (CREG) in Chinese with polymerase chain reaction with sequence specific primers (PCR SSP).
方法应用嵌套式逆转录聚合酶链反应技术检测印记基因p57kip2、LIT1、TSSC3在人类卵母细胞及植入前胚胎的正常表达。
Methods Using nested reverse transcription-PCR to analyze the expression of P57KIP2, LIT1, TSSC3 in human oocytes and preimplantation embryos.
方法采用病例对照分子流行病学研究方法和聚合酶链反应技术，检测89例原发性胃癌病例和94例对照GSTM 1和GSTT1基因型。
Mehtods a case control study was carried out and polymerase chain reaction (PCR) technique was used to identify GSTM1, GSTT1 genotype in 89 cases of primary gastric cancer and 94 controls.
方法运用序列特异性引物聚合酶链反应技术，检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的HLA-DQB1等位基因。
METHODS HLA-DQB1 gene polymorphisms were typed by sequence specific primer based polymerase chain reaction, in 42 patients with esophageal neoplasm and 136 normal control subjects.
方法：应用多聚合酶链反应技术(PCRT)，对10份遗传性卵巢癌组织中BRCA1基因内部的D17S855微卫星位点进行LOH检测。
Methods: Using the polymerase chain reaction technique (PCRT), LOHs in 10 samples of hereditary ovarian cancer at intragenic loci were detected.
现行的多元聚合酶链反应(PCR)技术最多只能在50种生物内进行一次检测。
Current multiplex polymerase chain reaction (PCR) techniques can at most offer detection from among 50 organisms in one test.
大多数分子标记体系的利用都取决于聚合酶链反应（PCR），这是一项扩增特定DNA序列的重要技术。
Use of most molecular marker systems depends on the polymerase chain reaction (PCR), which is an important technique for amplifying specific DNA sequences.
方法：采用聚合酶链反应(PCR)技术，检测了64例肺结核患者的外周血与痰标本结核杆菌dna。
Methods: Polymerase chain reaction (PCR) was adopted in detection of DNA of mycobacterium tuberculosis from peripheral blood and sputum in 64 tuberculosis patients.
其技术主要包括胚胎活检、聚合酶链反应及荧光原位杂交。
The basic techniques currently used involve embryo biopsy, the polymerase chain reaction and fluorescence in situ hybridization.
采用聚合酶链反应（PCR）技术和光密度扫描检测两组线粒体DNA的缺失突变情况。
Deletion mutation state of the two groups of mitochondria DNA were detected by PCR technology and photodensity scan.
目的：利用聚合酶链反应定点突变技术构建人血小板反应素1基因第13外显子编码钙结合域突变体。
AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.
需要使用包括聚合酶链反应（PCR），琼脂糖凝胶电泳，探针的标记与检测等生物工程技术。
This technique employs standard operations of biomedical engineering, such as polymerase chain reaction(PCR), agarose gel electrophoresis, labeling and detecting of probe.
目的探讨荧光定量聚合酶链反应（FQ-PCR）技术诊断结核病临床应用价值。
Objective To evaluate the clinical value of fluorescence quantitative PCR(FQ-PCR) technique in diagnosing tuberculosis.
方法采用单管四重聚合酶链反应(PCR)技术。
Methods Single barrel four-seat PCR technique was performed.
聚合酶链反应(PCR)技术用于病毒核酸的检测具有灵敏度高，特异性强等优点，已广泛用于各型肝炎病毒的实验室检测。
Polymerase Chain Reaction (PCR) technique has been widely used in detecting different hepatitis viruses in laboratory, because of its high specificity and sensitivity.
方法在140名汉族健康人的外周血中，应用聚合酶链反应（PCR）限制性片段长度多态性分析（RFLP）及多重PCR技术，进行NAT1等位基因分型研究。
Methods Using multiple PCR and PCR-RFLP methods, we studied the NAT1 genotypes and its genetic polymorphisms of the peripheral blood samples from 140 Han people.
方法：采用逆转录聚合酶链反应(RT - PCR)、流式细胞仪和免疫荧光细胞染色分析技术分析肺上皮细胞系a549细胞PAR s的表达情况。
METHODS: We used RT-PCR, flow cytometry and immunofluorescence cell staining techniques to observe the expression of PARs on A549 cells.
方法：用甲基化敏感的限制性核酸内切酶消化，结合聚合酶链反应（PCR）技术。
Methods: 20 cases of MDS patients were studied using methylation sensitive restriction enzyme digestion and polymerase chain reaction(PCR) technique.
目的探讨聚合酶链反应(pcr)加反相杂交技术在细菌DNA检测中的应用。
Objective To develop a molecular biologic technique for detection of bacterial DNA in all bacteria with PCR and reverse hybridization.
方法应用聚合酶链反应（ PCR）、DNA测序和限制性片段长度多态性（RFLP）等技术对1个帕金森病家系及120例散发性帕金森病患者进行PINK1基因R492X的突变分析。
Methods One family and 120 sporadic patients with Parkinson's disease were studied using polymerase chain reaction, DNA sequencing and restriction fragment length polymorphic (PCR-RFLP) techniques.
方法采用聚合酶链反应（PCR）技术进行淋菌、沙眼衣原体、解脲脲支原体3种常见病原体检测。
METHODS Polymerase chain reaction(PCR) technology was taken to detect out Neisseria gonorrhoeae, Chlamydia trachomatis and Ureaplasma urealytium.
方法应用聚合酶链反应限制性片段长度多态性技术检测296例两个基因多态位点的等位基因、基因型。
MethodGenotypes and alleles of polymorphisms of both genes were determined with polymerase chain reactionrestriction fragment length polymorphism assay (PCRRFLP) of 296 subjects in Han Chinese.
方法采用微量稀释法检测21种抗菌药物的敏感性，运用聚合酶链反应（PCR）技术检测氨基糖苷类修饰酶基因。
METHODS The dilution test was performed to detect the susceptibility to 21 kinds of antimicrobial agents and genotypes of AMEs of 20 E. coli strains were analyzed by polymerase chain reaction(PCR).
方法采用微量稀释法检测21种抗菌药物的敏感性，运用聚合酶链反应（PCR）技术检测氨基糖苷类修饰酶基因。
METHODS The dilution test was performed to detect the susceptibility to 21 kinds of antimicrobial agents and genotypes of AMEs of 20 E. coli strains were analyzed by polymerase chain reaction(PCR).
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