基于中蜂囊状幼虫病病毒基因组序列AF469603中的RNA依赖的RNA聚合酶基因，建立该病毒的半套式RT-PCR检测方法。
A specific semi-nested RT-PCR based on the RNA-dependent RNA polymerase gene in AF469603 of Chinese bee (Apis sinensis) sacbrood virus was established to detect Chinese bee sacbrood virus.
RNA病毒的聚合酶基因总体上和宿主密码子使用类型不一致，限制了聚合酶基因的及早和过高表达，但其密码子使用频率对聚合酶的限制是适中的。
RNA polymerase in RNA virus is different from host cells restrict it's high and early expression. The restrict is moderate compared with other virus genes.
更重要的是，后者激发了两倍的聚合酶——大约14个——塞满了整条基因，不停制造mRNA。
More significantly, the latter group of cells recruited twice as many polymerase enzymes - about 14 - which crammed along the gene's length, all producing mRNA.
有各种逆转录酶聚合酶链反应(扩增核糖核酸基因组RT–PCR)检测试验方法，但灵敏度各不相同。
Various reverse transcriptase–polymerase chain reaction (RT–PCR) methods are available but are of variable sensitivity.
应用一种缓慢但高度准确的试验，检测病毒的基因物质，叫即时逆转录-聚合酶链反应，或是rRT - P CR，全部做了评价。
All had been evaluated using a slower but highly accurate test called real-time reverse transcription-polymerase chain reaction, or rRT-PCR, which checks for the genetic material of the virus.
H5N 1中最重要的蛋白质可能是RNA聚合酶，它使病毒以所有自身基因物质而完成自我复制。
In H5N1, perhaps the most important of these proteins is RNA polymerase, which contains the instructions that allows the virus to copy itself along with all of its genetic material.
例如，RNA聚合酶ii发现集中在成千上万个基因的促销员，导致在该领域的极大兴趣，在调节基因表达的重点转移到伸长的作用。
For example, RNA polymerase II is found concentrated at the promoters of thousands of genes, causing much interest in the field to shift focus to the role of elongation in regulating gene expression.
方法采用逆转录—聚合酶链反应(RT -PCR)方法检测40例患者的肺癌组织ttf - 1基因的表达。
Methods 40 patients with lung cancer were studied to detect TTF-1 gene by using reverse transcription-polymerase chain reaction assay (RT-PCR).
通过DNA聚合酶反应，获得了改造的荧光假单孢菌脂肪酶基因。
Through DNA polymerase reaction, a modified lipase gene was obtained.
用逆转录-聚合酶链反应(RT - PCR)方法检测主动外排泵基因CDR1和CDR2的表达水平。
Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to measure the mRNA levels of active efflux gene CDR1 and CDR2.
目的：通过在大肠杆菌中分段表达禽流感病毒聚合酶酸性蛋白(PA蛋白)，探索PA基因中可能影响表达的区域。
Objective: To study the effect of different fragments of PA gene on the expression of PA protein of avian influenza virus in Escherichia coli.
方法提取人外周血单个核细胞总RNA，反转录聚合酶链反应（RT-PCR）扩增目的基因；
Methods RNA from human peripheral blood mononuclear cells were extracted and target genes were amplified by RT-PCR.
RNA聚合酶突然被启动，沿着DNA上升并读取基因。
Suddenly, RNA polymerase is let go, raising along the DNA to read the gene.
采用单链聚合酶链反应(PCR)和测序法检测肿瘤组织中VHL基因的突变情况。
Mutation of VHL gene from tumor tissue was detected from tumor tissue by polymerase chain reaction (PCR) and direct sequencing.
目的：利用聚合酶链反应定点突变技术构建人血小板反应素1基因第13外显子编码钙结合域突变体。
AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.
将位于多巴胺运输器基因3'端未转译区段的40 -碱基多形性重复序列予以纯化、经聚合酶链锁反应放大。
A 40-base polymorphic repeat sequence located in the 3 '-untranslated region of the DAT gene was purified and amplified by polymerase chain reaction (PCR).
方法：采用逆转录-聚合酶链反应(RT - PCR)检测48例胃癌组织及30例正常组织中vegf基因的表达。
Methods: The expression of VEGF gene in 48 samples of human gastric carcinomas and 30 samples of corresponding normal tissue specimens was detected by RT-PCR.
应用聚合酶链反应得到2株蓝绿藻的毒素聚酮合成酶(PKS)基因，并进行基因序列分析。
Gene of polyketide synthase (PKS) from 2 toxin-producing cyanobacteria was prepared by polymerase chain reaction and the gene sequence was analyzed.
方法应用实时监测聚合酶链反应检测这三种受体基因在雌激素受体阳性和雌激素受体阴性乳腺癌细胞系中的表达。
Methods These three genes were quantified by real-time quantification polymerase chain reaction (PCR) in er positive and er negative breast cancer cell lines.
方法：采集2000 ~ 2001年肾综合征出血热(HFRS)患者的血液标本，通过逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒M片断基因。
Methods: the blood samples were collected from HFRS patients between 2000 ~ 2001, and the m segment genome of hantavirus was amplified by RT-PCR.
方法：采用多重聚合酶链反应方法，以提取的基因组d NA为模板，在同一反应管中同时扩增细胞周期素d 1基因和P 16基因。
METHODS: Method of multiplex polymerase chain reaction (PCR) was adopted. Cyclin D1 gene and P16 gene were amplified in the same tube, using extracted genome DNA as template.
方法采用聚合酶链反应扩增该家系患者和健康对照个体atp2c1基因的全部外显子，直接测序法进行DNA测序，100例无亲缘关系的正常人作为对照。
Method all exons of ATP2C1 gene were analyzed with polymerase chain reaction and DNA sequencing in all patients of this family and 100 unrelated population-match controls.
从RNA聚合酶顶端延伸出的黄色链便是RNA，它是基因信息的一个副本。
The yellow chains sticking out of the top is the RNA, a copy of the genetic message.
方法采用病例对照分子流行病学研究方法和聚合酶链反应技术，检测89例原发性胃癌病例和94例对照GSTM 1和GSTT1基因型。
Mehtods a case control study was carried out and polymerase chain reaction (PCR) technique was used to identify GSTM1, GSTT1 genotype in 89 cases of primary gastric cancer and 94 controls.
方法采用聚合酶链反应-限制性片段长度多态性分析(PCR - RFLP)检测136例HBVDNA阳性的上海籍HBV感染者的基因型。
Methods HBV genotypes were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism analysis (RFLP) in 136 HBV DNA positive patients who were born in Shanghai.
方法对活禽市场分离的3株H3N8亚型鸭源流感病毒聚合酶PB1基因进行了序列分析。
Methods To illustrate the relationship of H3 subtype influenza virus with other subtypes, we carried out the sequence analysis of PB1 polymerase genes of three H3N8 subtype du.
家蚕细小病毒样病毒；基因组；DNA聚合酶；克隆；表达。
Bombyx mori parvo-like virus; Genome; DNA Polymerase; Cloning; Expression.
方法应用聚合酶链反应限制性片段长度多态性分析方法(PCR -RFLP)对40例口腔鳞癌组织中apc基因的杂合缺失(LOH)进行检测。
Methods We used the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) to examine 40 oral squamous cell carcinomas for loss of heterozygosity (LOH) at APC.
结合挖掘结果，运用网络结构可视化思想构建心气虚证网络结构图，并运用逆转录聚合酶链反应(RT - PCR)探索心气虚证基因网络结构。
To integrate the results of clustering data mining and based on visual model requirement, network structure diagram of HQDS would be drawn, gene network structure be study with RT-PCR.
结合挖掘结果，运用网络结构可视化思想构建心气虚证网络结构图，并运用逆转录聚合酶链反应(RT - PCR)探索心气虚证基因网络结构。
To integrate the results of clustering data mining and based on visual model requirement, network structure diagram of HQDS would be drawn, gene network structure be study with RT-PCR.
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