外源性核酸为模板的活性胶实验显示聚合酶和逆转录酶活性无变化。
But the activities of DNA polymerase and reverse transcriptase were found to remain active by activity gel with exogenous templates.
再次检查合成出的产物并更正其中错误的过程。dna聚合酶和rna聚合酶都具有一定的校正能力，以防止所合成的链中存在错误。
The process of rechecking work and correcting errors. DNA polymerase and RNA polymerase both have some ability to proofread the strands they are synthesizing.
他进一步解释道：“我要问，我能看到不同的人类DNA聚合酶(蛋白质)系列和另一种大肠杆菌DNA聚合酶序列的可能性有多少吗？”
"I asked, What's the probability that I would see a human DNA polymerase [protein] sequence and another protein with an E. coli DNA polymerase sequence?" he explained.
依据聚合酶链反应(PCR)和酶联免疫吸附检测(ELISA)的方法目前被应用于主要食源性致病菌的检测。
Polymerase chain reaction-based (PCR-based) and enzyme-linked immunoabsorbent assay (ELISA) methods are now applied in the detection of major food-borne pathogens.
原来是，如果您使用我的假设，即人类和大肠杆菌的DNA聚合酶其实是相关的，那么其概率就会高得多。
It turns out that probability is much higher if you use the hypothesis that [humans and E. coli] are actually related.
经过240周的治疗后，HBV聚合酶的蓄聚率、病毒的耐药率和临床耐药率分别是29%，20%和11%。
After 240 weeks of therapy, the cumulative rates of HBV polymerase mutations, virologic resistance, and clinical resistance were 29%, 20%, and 11%, respectively.
对单分子PCR产物的错误率进行了理论分析，结果表明：根据实验目的和条件，选择忠实性不同的聚合酶是十分关键。
The error rates of single molecule PCR under different conditions showed that it is essential to select different polymerases according to different experimental p.
RNA病毒的聚合酶基因总体上和宿主密码子使用类型不一致，限制了聚合酶基因的及早和过高表达，但其密码子使用频率对聚合酶的限制是适中的。
RNA polymerase in RNA virus is different from host cells restrict it's high and early expression. The restrict is moderate compared with other virus genes.
讨论了O 抗原糖基转移酶和聚合酶对底物的特异性；
The substrate specificity of O-antigen glycosyltransferases and polymerases are proposed.
但是研究人员不清楚的是聚合酶是怎样避免把构成DNA链的NTP添加到RNA链上的，毕竟脱氧核核苷酸和核糖核苷酸只是相差一个氧原子。
And the scientists didn't know either how the polymerase avoids substituting the NTPs that constitute DNA for the correct RNA building blocks, molecules that differ by only one oxygen atom.
用逆转录-聚合酶链反应(RT - PCR)方法检测主动外排泵基因CDR1和CDR2的表达水平。
Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to measure the mRNA levels of active efflux gene CDR1 and CDR2.
方法：采用多重聚合酶链反应方法，以提取的基因组d NA为模板，在同一反应管中同时扩增细胞周期素d 1基因和P 16基因。
METHODS: Method of multiplex polymerase chain reaction (PCR) was adopted. Cyclin D1 gene and P16 gene were amplified in the same tube, using extracted genome DNA as template.
采用单链聚合酶链反应(PCR)和测序法检测肿瘤组织中VHL基因的突变情况。
Mutation of VHL gene from tumor tissue was detected from tumor tissue by polymerase chain reaction (PCR) and direct sequencing.
DNA饱和分析法，是一种简便的测定rna聚合酶相对含量和相对比活性的方法。
DNA saturation experiment is a convenient method for measuring the relative amount and specific activity of RNA polymerase.
聚合酶链反应(PCR)可作为一种快速检测并鉴别马疱疹病毒1型(EHV 1)和马疱疹病毒4型(EHV 4)的诊断方法。
A rapid method for detection and identification of equine herpesvirus 1 (EHV-1) and equine herpesvirus 4 (EHV-4) was developed using polymerase chain reaction (PCR).
还有核苷和聚合酶。
DNA聚合酶可以校对和修正，但是RNA聚合酶没有这种能力。
DNA polymerases are capable of editing and error correction, but RNA polymerases do not appear to have this capacity.
方法应用实时监测聚合酶链反应检测这三种受体基因在雌激素受体阳性和雌激素受体阴性乳腺癌细胞系中的表达。
Methods These three genes were quantified by real-time quantification polymerase chain reaction (PCR) in er positive and er negative breast cancer cell lines.
应用反向间接血凝试验（RPHA）、酶免疫测定（EIA）和聚合酶链反应（PCR）测定了家禽血清、卵黄和牛乳清中HBV标志物及人HBV-DNA。
The HBV markers and HBV DNA in poultry sera, Yolk and bovine milk whey were detected by reversed passive hemagglutination assay (RPHA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR).
采用聚合酶链反应（PCR）技术和光密度扫描检测两组线粒体DNA的缺失突变情况。
Deletion mutation state of the two groups of mitochondria DNA were detected by PCR technology and photodensity scan.
抑制聚腺苷二磷酸核糖聚合酶对休克和缺血再灌注损伤有良好的防治作用。
Inhibition of poly (ADP-ribose) polymerase has preventive and therapeutic effects on the treatment of shock and ischemia-reperfusion injury.
新的快速检测技术有聚合酶链式反应(PCR)技术、伏安型生物传感器、自动微生物检测系统(ams)、改良MRS培养基和三磷酸腺苷(atp)法等，较传统方法迅速、准确。
The new rapid determination techniques were polyase chain reaction method (PCR), volt-ampere biosensor method, automatic microbe system method (AMS), improvement of MRS culture medium and ATP etc.
方法：采用序列特异性引物聚合酶链反应的方法对汉族72例uc患者和314例正常对照者HLA - DRB1基因分型。
Methods: By using polymerase chain reaction-sequence specific primers, frequency of HLADRB1 alleles in 72 patients with UC and 314 healthy controls were detected.
目的：应用内转录间隔区(its)通用引物建立一种较为快速、简便的检测和鉴定念珠菌的聚合酶链反应(PCR)方法。
Objective: To set up a rapid and simple PCR assay for Candida detection and identification by using internal tran scribed spacer (ITS) universal primers.
采用反转录-聚合酶链反应检测两组大鼠大网膜和皮下脂肪组织中抵抗素基因的表达。
The RT-PCR was adopted to detect the expressions of resistin gene in epiploon and subcutaneous fatty tissues.
方法采用病例对照分子流行病学研究方法和聚合酶链反应技术，检测89例原发性胃癌病例和94例对照GSTM 1和GSTT1基因型。
Mehtods a case control study was carried out and polymerase chain reaction (PCR) technique was used to identify GSTM1, GSTT1 genotype in 89 cases of primary gastric cancer and 94 controls.
方法对医院临床分离的8株耐亚胺培南铜绿假单胞菌进行随机引物聚合酶链反应（PCR）扩增，扩增产物进行电泳和聚类分析。
METHODS The genes of imipenem-resistant P. aeruginosa were amplified by RAPD assay in 8 clinical isolates and PCR products were analyzed by agarose gel electrophoresis and cluster analysis.
采用聚合酶链反应(PCR)方法扩增rho基因第1 ~ 5外显子和第1内含子基因片段，用直接dna测序法筛查rho基因突变。
Extron 1-5 of RHO gene was amplified by polymerase chain reaction (PCR), and the mutation of RHO gene was screened by direct DNA sequence measurement.
方法：应用聚合酶链反应（PCR）-双酶切法，对23例CMT1患者和30例正常人进行基因特异性连接片段的检测。
METHODS:Polymerase chain reaction(PCR) combined with restriction enzyme digest ion were used to detect gene specific junction fragments of the 23 CMT1 patients and 30 normal controls.
方法：应用聚合酶链反应（PCR）-双酶切法，对23例CMT1患者和30例正常人进行基因特异性连接片段的检测。
METHODS:Polymerase chain reaction(PCR) combined with restriction enzyme digest ion were used to detect gene specific junction fragments of the 23 CMT1 patients and 30 normal controls.
应用推荐