结论羊膜组织和培养羊膜细胞中有神经干细胞特异性标记蛋白的表达。
ConclusionSpecific mark proteins of neural stem cell are expressed in human amnion tissue and cultured amniotic cells.
目的探讨羊膜细胞培养液治疗兔眼重度碱烧伤的机制，为临床应用提供理论依据。
Objective To investigate the therapeutic mechanism of extract of human amniotic cells in treating rabbit corneal alkali burn and to provide the rational evidence for its clinical application.
方法采用组织块培养法培养羊膜细胞，酶消化法对细胞进行传代，应用免疫细胞化学的方法对细胞进行鉴定。
Methods Amnion cells from human were cultured with tissue piece method, passaged by trypsin digestion and identified with immunocytochemistry.
通过试验发现，WT在各试验浓度对人羊膜细胞株FL、小鼠纤维母细胞株L929无明显的抑制增殖的作用；
Part Two Toxicity of WT in Vitro The study used MTT method to detect the toxic activity of WT on FL cell line, L929 cell line and the spleen cells of mice.
目的建立羊膜组织共聚焦激光扫描显微镜光学切片的方法，探讨早期人胚羊膜细胞结构、分层及细胞骨架的空间结构。
Objective to develop a method for the optical section of laser scanning confocal microscopy of amnion and to study the spatial structure of cell and actin cytoskeleton in amnion of human embryo.
电镜下见成纤维细胞胞膜基底部向HA-ECM内伸出数个突起，细胞与羊膜基质粘附相当牢固，胞外有较多胶原原纤维堆积。
The basal membrane of fusiform fibroblasts with oval nucleus and rich in chromatin reached much protuberance into matrix of HA ECM and collagen fibril cumulating outside cells.
对照侧上皮细胞的凋亡较羊膜移植侧明显。
Apoptosis of epithelium cells in control was more than in amniotic membrane transplantation.
结论：（1）羊膜基底膜可作为筛选某些肿瘤细胞亚系的有效侵袭滤膜；
We conclude that(1) Amniotic basement membrane can be used as an effective invasive filter for the selection of some tumor cell sublines.
结果纤维软骨细胞于羊膜上牢固粘附，3天开始增殖，2周时已有大量细胞生长于羊膜上。
Results Fibrocartilage cells attached to amnion closely and begin proliferate after 3d, 2 weeks after had lots of cells proliferate onto amnion.
目的：探讨深低温冷冻保存角膜缘干细胞羊膜片的活性及异体移植疗效。
Objective:To investigate the activation of cryopreserved corneal limbus epithelium on the upper parts of amnion and the effect of hetero grafting.
结论1. MC3T3 - E1细胞在羊膜基质上以常规培养方法培养可以存活3周，并聚集成簇复层排列，相互沟通。
Conclusion: 1. MC3T3-E1 cells can survival on the HAAM under routine culture for 3 weeks, and migrated into clusters with communication.
目的：探讨人羊膜上皮细胞和羊膜间质细胞的分离、培养及鉴定。
Objective: To investigate the isolation, culture and identification of human amniotic epithelial cells and mesenchymal cells.
目的观察自体角膜缘干细胞移植或羊膜移植治疗翼状胬肉的临床效果。
Objective To observe the clinical effect of autograft limbal stem cell transplantation or amniotic membrane transplantation for pterygium.
目的探讨人羊膜上皮细胞移植治疗恒河猴脊髓半切损伤的作用。
Objective to explore the effects of human amniotic cells transplantation into Rhesus monkeys with spinal cord injury.
生物羊膜联合带自体角膜缘干细胞的结膜瓣移植治疗伴睑球粘连的复发性翼状胬肉是一种较理想的治疗方法。
Biological amnion combined with conjunctival flap with limbus stem cell transplant in the treatment of recurrent pterygium complicated with symblepharon was an ideal therapeutic method.
目的比较角膜缘干细胞移植术和羊膜移植术治疗复发性翼状胬肉的临床疗效。
Objective to compare the therapeutic effects of recurrent pterygium treated by limbal stem cell autograft transplantation and amniotic membrane transplantation.
结论人羊膜上皮细胞在体外可成功进行原代和传代培养，体外培养的人羊膜上皮细胞在一定时间内可维持增殖能力。
Conclusion Human amniotic epithelial cells can be successfully primary cultured and passaged in vitro, and the cells can proliferate for a certain period.
目的：用体外培养的角膜缘上皮细胞联合人羊膜的方法，行自体移植治疗角膜缘干细胞完全缺乏的兔眼。
Objective: to study in vitro cultivated limbal epithelial cells autograft with amniotic membrane grafts for total limbal stem cell deficiency of rabbit eyes.
目的：探讨体外培养人羊膜间充质细胞的神经生物学特征。
Objective: To evaluate the neurobiological features of human amnion mesenchymal cells in vitro.
目前研究主要集中在胎盘不同部位分离的间充质基质细胞和从羊膜分离的上皮细胞。
Most of the research published in this area focuses on mesenchymal stromal cells isolated from various parts of the placenta or epithelial cells isolated from amniotic membrane.
结论羊膜能促进结膜杯状细胞的生长及分化，是一种良好的结膜重建材料。
Conclusions Amniotic membrane helps to the growth and transdifferentiation of goblet cell and...
目的探索羊膜组织来源的细胞向神经细胞分化的可能性，为神经移植探寻新的细胞来源。
Objective To search for culture conditions in which the cells from human amnion could differentiate into neural cells and hence to explore a new cell source for neural transplantation.
目的建立体外培养人羊膜上皮细胞的方法，观察体外培养的羊膜上皮细胞的生物学特性。
Objective to establish a method of culturing human amniotic epithelial cells in vitro and to study its biologic features.
目的观察人羊膜移植联合自角膜缘干细胞移植治疗中重度眼部碱烧伤的临床效果。
ObjectiveTo observe the clinical effects of amniotic membrane transplantation and combination stem cell of self-limbal graft for medium and severe ocular alkaline burn.
结果人羊膜上皮细胞可以在体外成功的培养传代，体外可连续传8 ~10代。
Results Human amniotic epithelial cells were successfully cultured and passaged in vitro, and could be passaged successively for 8-10 times.
目的观察以羊膜为载体的兔角膜缘干细胞膜片移植治疗兔角膜缘干细胞缺损的效果。
Objective to study the treatment of rabbit limbal stem cell deficiency by cultured limbal stem cells growing on amniotic membrane for autograft transplantation.
结论：人羊膜是角朊细胞在体外培养的良好载体，对角朊细胞有明显的促增殖作用。
CONCLUSION: Human amitotic membrane is a good carrier of keratinocytes cultured on it in vitro, and is able to promote the proliferation of keratinocytes significantly.
目的探讨羊膜为载体培养的胎兔角膜缘干细胞移植治疗兔角膜缘碱烧伤的效果。
Objective To evaluate the effect of transplantation for treatment of rabbit limbal alkali burn by cultured limbal stem cells of fetal rabbits growing on amniotic membrane ex vivo.
目的：探索羊膜基底膜对结膜上皮细胞形态功能的影响。
Objective: To study the influence of amniotic membrane on the morphology and function of cultured human conjunctival epithelial cells.
目的：探索羊膜基底膜对结膜上皮细胞形态功能的影响。
Objective: To study the influence of amniotic membrane on the morphology and function of cultured human conjunctival epithelial cells.
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