目的探讨人羊膜上皮细胞移植治疗恒河猴脊髓半切损伤的作用。
Objective to explore the effects of human amniotic cells transplantation into Rhesus monkeys with spinal cord injury.
目的：探讨人羊膜上皮细胞和羊膜间质细胞的分离、培养及鉴定。
Objective: To investigate the isolation, culture and identification of human amniotic epithelial cells and mesenchymal cells.
目的：探讨羊膜上皮细胞是否能在体外促进胚胎脑神经干细胞的存活及分化。
Objective Whether amniotic epithelial cells could promote the survival and differentiation of neural stem cells was explored in vitro.
结果人羊膜上皮细胞可以在体外成功的培养传代，体外可连续传8 ~10代。
Results Human amniotic epithelial cells were successfully cultured and passaged in vitro, and could be passaged successively for 8-10 times.
目的建立体外培养人羊膜上皮细胞的方法，观察体外培养的羊膜上皮细胞的生物学特性。
Objective to establish a method of culturing human amniotic epithelial cells in vitro and to study its biologic features.
结论人羊膜上皮细胞在体外可成功进行原代和传代培养，体外培养的人羊膜上皮细胞在一定时间内可维持增殖能力。
Conclusion Human amniotic epithelial cells can be successfully primary cultured and passaged in vitro, and the cells can proliferate for a certain period.
对照侧上皮细胞的凋亡较羊膜移植侧明显。
Apoptosis of epithelium cells in control was more than in amniotic membrane transplantation.
目的：用体外培养的角膜缘上皮细胞联合人羊膜的方法，行自体移植治疗角膜缘干细胞完全缺乏的兔眼。
Objective: to study in vitro cultivated limbal epithelial cells autograft with amniotic membrane grafts for total limbal stem cell deficiency of rabbit eyes.
目的：观察羊膜对在其上皮面培养的兔角膜上皮细胞增生的影响。
Objective: To investigate the effects of amniotic membrane on the proliferation of the rabbit's corneal epithelium cultured on it.
目前研究主要集中在胎盘不同部位分离的间充质基质细胞和从羊膜分离的上皮细胞。
Most of the research published in this area focuses on mesenchymal stromal cells isolated from various parts of the placenta or epithelial cells isolated from amniotic membrane.
目的：探索羊膜基底膜对结膜上皮细胞形态功能的影响。
Objective: To study the influence of amniotic membrane on the morphology and function of cultured human conjunctival epithelial cells.
结果：倒置显微镜观察，悬浮细胞培养法A组和组织块培养法B组扩增的角膜缘上皮细胞均能在羊膜上单层生长。
Results: A monolayer of cultured corneal limbal epithelial cells was observed by inverted microscope in both A group and B group.
结论：羊膜有促进兔角膜上皮细胞增生的作用，可用做角膜表面结构重建的理想材料。
Conclusion: Amniotic membrane has the effect on enhancing the proliferation of the rabbit's corneal epithelium, so it can be used as an ideal material for the reconstruction of corneal surface.
结论：羊膜有促进兔角膜上皮细胞增生的作用，可用做角膜表面结构重建的理想材料。
Conclusion: Amniotic membrane has the effect on enhancing the proliferation of the rabbit's corneal epithelium, so it can be used as an ideal material for the reconstruction of corneal surface.
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