目的探讨n -甲基- D -天冬氨酸(NMDA)对大鼠视网膜神经节细胞层(RG CL)神经元的毒性作用。
Objective to investigate the neurotoxic effect of N-methyl-D-aspartate on neurons of retinal ganglion cell layer in rat.
VIP表达主要位于视网膜光感受器外节、内核层、内网状层和脉络膜，神经节细胞层和神经纤维层也有弱表达；
The expression of VIP exists on photoreceptor- outer segment, inner nuclear layer and inner plexiform layer of retina and choroids, and weak expression exists on ganglion cell and nerve fiber layer.
然后进行视网膜切片，计数视神经节细胞层细胞，定量视神经损伤。
The retina was sectioned for counting the cells in the ganglionic layer of the optic nerve and quantitating optic nerve lesion.
TUNEL观察结果：糖尿病3及6个月组视网膜发现TUNEL阳性细胞，主要集中于神经节细胞层及内核层，外核层未发现阳性细胞。
TUNEL: there were positive expression in 3 month group and more positive expression in 6 month group, and the TUNEL cells were in the ganglion cells and the inner nuclear layers.
TUNEL观察结果：糖尿病3及6个月组视网膜发现TUNEL阳性细胞，主要集中于神经节细胞层及内核层，外核层未发现阳性细胞。
TUNEL: there were positive expression in 3 month group and more positive expression in 6 month group, and the TUNEL cells were in the ganglion cells and the inner nuclear layers.
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