而构建缺氧培养环境作为确立细胞缺氧损伤模型实验的基础工作是极其重要的。
And as the basic for the whole experimental work, designing the model of hypoxia cell culture is very important.
目的：探讨外源性a20基因在缺氧培养条件下对内皮细胞e -选择素表达的影响。
Objective: To observe the effect of exogenous A20 gene on hypoxia-induced E-selectin expression of endothelial cells.
目的观察脑溢安血清对体外缺氧培养的大鼠脑微血管内皮细胞血管内皮生长因子（VEGF）基因表达的影响。
Objective To study the effects of Nao Yi An(NYA) serum on the expression of vascular endothelial growth factor(VEGF) gene in cultured rat cerebral microvascular endothelial cell(BMEC)with hypoxia.
目的探讨淫羊藿甙对体外原代培养神经元缺氧缺糖损伤的作用。
Objective To investigate the effects of Icariin on anoxic and glucose-deprived injury in primary cultured neurons.
目的利用氟桂利嗪(FNZ)研究钙离子拮抗剂对体外培养海马神经元缺血缺氧损伤的作用及其作用机制。
Objective To study the dual effects of calcium antagonists on ischemic hypoxic injury of hippocampal neurons cultured in vitro by using flunarizine (FNZ) and the mechanism of its action.
培养的第2～3代细胞适用于缺氧实验和药物作用机制的研究。
Cultured pulmonary trunk wall cells at the 2nd to 3rd passage were applicable to investigations of hypoxic experiments and efficiency of drugs.
目的：观察缺氧条件下血管紧张素-2对培养的血管内皮细胞分泌内皮素的影响及茶多酚的保护作用。
Objective:To observe changes of expression level of endothelin in endothelial cell after exposure to angiotensin-2 in hypoxia and investigate the protection manchanism of tee polyphenols.
目的建立体外培养大鼠皮质及海马神经元缺氧缺糖模型。
Objective To establish oxygen-glucose deprivation model of rats′ cortex and hippocampusal neurons in vitro.
目的：通过建立体外培养的人视网膜色素上皮细胞模型，观察缺氧对该细胞的影响；
ObjectiveTo establish the model of human retinal pigment epithelium(RPE) cells in vitro culture and investigate the cells under hypoxia.
应用免疫细胞化学技术研究BDNF、GDNF蛋白在体外培养正常神经干细胞和缺氧损伤神经干细胞中的表达以及脑溢安的影响。
Using immunocytochemistry to study the expression of BDNF and GDNF in normal NSC and NSC that had been damaged by hypoxia.
目的：建立耳蜗器官体外培养模型，观察缺氧对体外培养耳蜗内毛细胞（IHC）及外毛细胞（OHC）的影响。
Objective To establish a practical model for Wistar rat cochlea organ cultivated in vitro and observe the effect of hypoxia on the inner hair cells(IHC) and outer hair cells(OHC).
目的探讨缺氧对体外培养成骨细胞增殖及凋亡的影响。
Objective to study the effect of hypoxia on osteoblast proliferation and apoptosis.
方法：采用酶消化法传代培养乳sd大鼠颅盖骨细胞并建立缺氧模型。
Methods: the osteoblasts obtained from the calvaria of neonatal SD rats were cultured by method of enzyme digestion in vitro.
将好氧污泥与厌氧污泥混合培养，在缺氧反应器中培养出了厌氧氨氧化细菌，实现了在缺氧反应器中进一步降解NH3-N的目标。
The mixture of aerobic and anaerobic sludge is used to culture denitrifying bacteria using ammonium as donors in anoxic reactor. The aim of NH3-N degradation in anoxic reactor is achieved.
结论缺氧具有抑制体外培养成骨细胞增殖，促进成骨细胞凋亡的作用。
ConclusionHypoxia can not only inhibit the proliferation of osteoblasts but also facilitate their apoptosis.
本文用新生大鼠下丘脑培养细胞，研究了低氧预处理对下丘脑细胞缺氧耐受性的影响及其与线粒体膜电位的关系。
The relationship between enhanced anoxic tolerance induced by hypoxic preconditioning and mitochondrial membrane potential (MMP) was studied in cultured hypothalamic cells.
目的：在神经元培养时制作实验性缺氧缺糖模型。
OBJECTIVE: To produce experimental OGD models in cultured neurons.
目的：探讨低分子肝素对体外培养的新生大鼠大脑皮层神经细胞缺氧性凋亡的作用机制。
AIM: to investigate the mechanism by which low molecular weight heparin (LMWH) inhibits apoptosis of primary cultured cerebral cortical neurons caused by hypoxia.
方法：采用原代培养心肌细胞，建立心肌细胞化学缺氧损伤实验模型。
Methods: The experimental model of chemical hypoxia was established by primary cultured myocardial cells.
用MTT法和混合共培养实验分别检测转基因细胞在缺氧或不缺氧状态下对GCV的敏感性以及HRE-TK/GCV系统的“旁观者效应”；
The sensitivity of transfected cells to GCV and "bystander effect (BSE)" of HRE-TK/GCV system under normoxia or hypoxia conditions were measured by MTT assay and mixed coculture experiment.
方法：将原代培养的乳鼠心肌细胞随机分为五组：正常对照组(N组)、缺氧对照组(H组)、缺氧加黄芪组1 (A 1组)、缺氧加黄芪组2 (A 2组)、缺氧加黄芪组3 (A 3组)。
Methods: Primary cultured neonatal rat myocardial cell were randomly divided into five group: normal control group (n), hypoxic group (h), AM 1 (A1), AM 2 (A2), AM 3 (A3).
介绍了缺氧-好氧-接触氧化法处理难降解焦化废水的工艺原理、污泥的培养驯化及试运行效果。
The combined biochemical process includes three sections: anaerobic biochemical reduction, aerobic biochemical oxidation and contact biological degradation.
介绍了缺氧-好氧-接触氧化法处理难降解焦化废水的工艺原理、污泥的培养驯化及试运行效果。
The combined biochemical process includes three sections: anaerobic biochemical reduction, aerobic biochemical oxidation and contact biological degradation.
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