目的：利用二维凝胶电泳法分离人肾组织磷酸化蛋白质组。
Objective: To separate human kidney phosphoproteome by two-dimensional gel electrophoresis.
用十二烷基硫酸钠-凝胶电泳(SDS -PAGE)和二维凝胶电泳鉴定蛋白质纯度。
The obtained proteins were checked for the purity through SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
所不同的是我们采用了优化的一维凝胶电泳和液相色谱串联质谱联合的复杂蛋白质混合物鉴定技术。
The difference of this chapter is that we use an optimized one-dimensional gel electrophoresis and liquid chromatography tandem mass spectrometry combined protein mixture identification technology.
常见的用于宿主细胞蛋白的分析的技术包括酶联免疫吸附法(酶联免疫吸附试验)，一维或二维凝胶电泳，免疫印迹和质谱分析。
Common technologies used for HCP analysis include ELISA (Enzyme-linked Immunosorbent Assay), one-dimensional or two-dimensional gel electrophoresis, Western Blot and Mass Spectrometry.
方法利用二维凝胶电泳获得新生隐球菌孵育后血管内皮细胞与正常细胞差异表达蛋白质点，并对部分差异蛋白点进行质谱鉴定分析。
Methods The differentially expressed proteins between HUVEC incubated with Cryptococcus and normal HUVEC were obtained by 2-D electrophoresis and identified by MALDI-TOF MS technology.
定量后，分别进行一维等电聚焦分离和二维聚丙烯酰胺凝胶电泳分离。
After quantification, the protein samples were separated by isoelectric focusing electrophoresis and then by SDS-PAGE.
定量后，分别进行一维等电聚焦分离和二维聚丙烯酰胺凝胶电泳分离。
After quantification, the protein samples were separated by isoelectric focusing electrophoresis and then by SDS-PAGE.
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