目的提出一个可供分离培养结核杆菌的简易血清琼脂斜面培养方法。
Objective To develop a simple serum-agar slant medium for cultivation and isolation of tubercle bacilli.
目的研究结核杆菌微菌落培养中细菌繁殖的周期。
Objective To study the reproductive cycle of bacteria in the culture of mycobacterium tuberculosis microcolony.
痰中结核杆菌的检测方法常规采用痰涂片及痰培养。
Methods TB was detected by smearing directly or smearing of concentrated sputum and by sputum culturing.
结果荧光定量pcr法检出结核杆菌阳性率显著高于痰涂片抗酸染色和培养法，其他非肺结核结果阳性率仅为2。
Results The positive rate of MTB detected with FQ-PCR was higher than that with smear acid-fast and culture of organism. The positive rate of non-tuberculosis was only 2.7%.
结果荧光定量pcr法检出结核杆菌阳性率显著高于痰涂片抗酸染色和培养法，其他非肺结核结果阳性率仅为2。
Results The positive rate of MTB detected with FQ-PCR was higher than that with smear acid-fast and culture of organism. The positive rate of non-tuberculosis was only 2.7%.
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