目的和方法:用噬菌体随机7肽库对神经胶质瘤母细胞(SWO - 38)进行全细胞筛选,并通过竞争结合实验检验所筛选出的克隆的结合特异性。
AIM and METHODS: The Ph. D. -7 phage display library was used to isolate peptides specific for glioma SWO-38 cell by whole cell screening.
抗体具有非常特异性,每种抗体只识别和结合一种蛋白质区域。
Antibodies are very specific in that each antibody will recognize and bind to one protein region only.
研究者们接下来通过特异性结合细胞表面蛋白质的抗体将神经胶质瘤细胞分成不同的类型。
The researchers then sorted glioma cells into different types using antibodies that stick to specific proteins on a cell's surface.
这件装置是一种HIV蛋白,它经过科学家的修改,可以只与病毒结合其感染细胞的部位特异性作用。
The device is an HIV protein that the scientists modified so it would react only with antibodies specific to the site where the virus binds to cells it infects.
生物素结合位点的复杂性和结构的不规则性使得它最适合与生物素结合并且表现出与链霉素亲和素-生物素复合体的高度特异性(4)。
The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the streptavidin-biotin complexes (4).
首先他们分离与DNA的启动序列特异性结合的转录因子。
First, they isolated transcription factors that were specifically bound to DNA at their promoter sites.
用直接和间接ELISA方法检测噬菌体阳性克隆与CTGF结合的特异性。
The specificity of phages combined with CTGF was identified by direct and indirect-ELISA.
利用溴甲酚绿与血清白蛋白的特异性结合,测定血清白蛋白的含量。
The specific combination of the bromocresol green (BCG) with serum albumin was made use of assaying for serum albumin.
方法利用抗原、抗体特异性结合原理,在组织和细胞中进行抗原、抗体准确定性、定位的检测。
Methods:Applying the principle of specific binding of antibody and antigen, make detection on accurate localization and qualitaliveness in tissues and cells.
此复合体中P23作为HSP90的伴侣分子,能够和HSP90特异性结合,调节HSP90与ATP或ADP结合的形式而达到结合或释放靶蛋白的功能。
P23, as one co-chaperone of HSP90, could bind to HSP90 specially, let HSP90 bind or release the target protein by regulating the form of the HSP90 binding with ATP or ADP.
用单克隆抗体作为酶结合物抗体可以提高实验方法的特异性。
The specificity of the assay can be improved by using monoclonal antibody as enzyme-linked antibody.
目的:从噬菌体呈现12肽库中筛选与流感病毒神经氨酸酶特异性结合的肽。
Objective: to select the peptide binding to influenza virus neuraminidase from phage display 12 peptide library.
叶酸在特异性结合蛋白——叶酸结合蛋白(fbp)的作用下,完成其吸收、转运、代谢等一系列生理过程。
The physiologic process of folic acid, including absorbing, transporting, and metabolism, were mediated by a specific binding protein-folate binding protein (FBP).
药物对映体结构特异性对局麻药药代动力学的各个过程都有着很大影响,包括药物的吸收、蛋白结合率、代谢等。
The enantiomer-specific structure of local anaesthetics has large impact on the pharmacokinetic process of these drugs, including absorption, protein-binding ratio, metabolism, and so on.
结论PNA与靶序列dna结合有高度的特异性,选用PNA作探针,可提高压电基因传感器检测的特异性。
Conclusion PNA combining with the DNA of target sequence had a highly specificity. Choosing PNA as the probe may improve the determined specificity of piezoelectric gene sensor.
病毒颗粒可以包裹一种多肽,能够和整合素特异性结合。
The virus particles could be coated externally with peptides that bind to integrins.
决定氨基酸残基和碱基相互关系的结合几何图形表示为立体化学图,它表明了识别的特异性。
The binding geometry determined by interaction of the residues and bases in recognition area is indicated in the stereochemical chart.
目的:研究凋亡因子TRAIL结合端粒酶启动子对肿瘤的特异性杀伤作用。
OBJECTIVE: to investigate the specificity role of the apoptosis factor TRAIL gene with telomerase promoter in tumor gene therapy.
测定时多将普适性量表和针对某一病种的特异性量表结合使用,采用自评量表法。
The common instrument and special instrument were used in assessment and the method of self-measuring scale was adopted.
前言:目的:观察中西医结合治疗慢性非特异性溃疡性结肠炎的疗效。
Objective: To observe the curative effect of the therapy of integrated TCM-WM in treating nonspecific ulcerative colitis.
以固相化的SARS抗原淘筛抗体库,并用ELISA检测噬菌体抗体结合SARS病毒的特异性。
Antibodies against SARS virus were screened by biopanning with immobilized virus antigen. The binding specificity of phage antibody to SARS virus was detected by ELISA.
结论:肌钙蛋白c2能特异性结合癌基因TRE17,为进一步研究TRE17的恶性转化机制提供了新的线索。
CONCLUSION: Troponin C2 specifically associates with TRE17 oncogene, which provides some new clues to make more researches on mechanism of TRE17 oncogene's transformation.
目的:鉴定自制的抗CD3-抗胶质瘤双特异性抗体(双抗)的双向结合能力,测定其免疫活性及其对T淋巴细胞的作用。
Objective: To study bidirectional binding ability and immunoactivity of anti-CD3/anti-glioma bispecific antibodies(BsAb) , and to study its effect on T lymphocytes cells.
结论:利用噬菌体抗体库技术筛选可得到与破伤风外毒素特异性结合的人抗破伤风类毒素基因工程抗体。
Conclusion: Human genetic engineering antibody, which is able to react specifically to tetanus toxin, is obtained through screening phage antibody library.
因此,通过膜连蛋白V或乳凝集素与凋亡细胞膜表面外翻的磷酯酰丝氨酸特异性结合,可用于冠状动脉易损斑块的早期检测。
The specific binding of phosphatidylserine exposed on the cell surfaces undergoing apoptosis with annexin V or lactadherin, can be used for early detection o.
目的:研究TRAIL基因结合端粒酶启动子特异性靶向治疗的作用。
AIM: To explore targeted gene therapy of tumor by using the combination of TRAIL gene with the telomerase promoter.
本文对检测血吸虫病抗体的胶乳凝集试验重新进行了评价,证明共价结合的日本血吸虫卵胶乳抗原有较高的敏感性和特异性。
Re-evaluation of latex agglutination test to detect the anti-schistosome antibody showed that the covalently bounded latex-egg antigen was highly sensitive and specific.
结果该双功能抗体与舌癌组织特异性结合率为85.29%,与血管内皮细胞结合率为82.35%。
Results: the specific conjugation rate between bifunctional antibody and lingual cancer tissue was 85.29%, and that between the antibody and vascular endothelial cells was 82.35%.
结论肺部韦格氏肉芽肿的CT表现缺乏特异性,结合临床资料综合分析可减少误诊。
Conclusion CT findings of Wegener's granulomatosis are nonspecific. It is essential for accurate diagnosis to correlate CT findings with clinical data.
结论肺部韦格氏肉芽肿的CT表现缺乏特异性,结合临床资料综合分析可减少误诊。
Conclusion CT findings of Wegener's granulomatosis are nonspecific. It is essential for accurate diagnosis to correlate CT findings with clinical data.
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