细胞贴壁率最高达60%左右。
该细胞贴壁生长,具有接触抑制的性质。
These cells were able to grow adhering to the wall and stop growing with contact inhibition.
体外培养观察软骨细胞贴壁和生长均较缓慢。
The in vitro observation showed that cell adherence and growth was slow.
复苏的细胞贴壁时间延迟,但培养72小时以后,细胞活力明显增强。
The activity of frozen cell was lower than fresh chondrocyte in the first 24h, but the activity was obviously enhanced after 72h.
通过计算细胞贴壁率检测BBS及其受体拮抗剂对细胞粘附能力的影响;
The effect of BBS and its receptor antagonist on cell spreading ability was detected by the percentage of cell adhesion.
而B组(弃掉表皮干细胞组)细胞贴壁相对缓慢,且形成的集落小而少。
But B set (leave the epidermis stem-cell set) the cell stick wall opposite and slow-moving, and formation of gather to fall small and little.
结果大鼠骨髓基质细胞贴壁呈集落生长,5 -氮胞苷诱导骨髓基质细胞转化为心肌样细胞。
Results Mesenchymal stem cells were spindle-shaped and active proliferation capacity in vitro, they can be differentiation into cardiac-like muscle cells after adding 5-azacytidine.
细胞增殖抑制试验采用细胞贴壁培养,乙醇固定,结晶紫染色,单层细胞密度计测定细胞密度。
Cell growth inhibition was tested by cell sticking cultivation, ethanol fixation, crystal violet staining and monolayer cell density assaying.
应用体外骨髓基质细胞贴壁培养的方法,观察了小鼠骨髓贴壁细胞的形态及其对造血的调控作用。
By using the techniques of in vitro bone marrow long-term liquid culture system, we observed the morphological features of murine bone marrow stroma cells and their hemotopo etic regulation.
利用生物化学和细胞培养方法对研制出的新型羧甲基壳多糖微载体CX-2的生物亲和性、细胞贴壁及生长和扩展特性进行了研究。
The properties of the newly developed microcarrier (MC) CX-2 were investigated using biochemical and cytological techniques, including bio-affinity, cyto-compatibility, eurytopicity, and so on.
提取的骨髓基质细胞呈贴壁生长,诱导后可向成骨细胞分化,细胞呈成骨细胞形态为梭形和多角形。
BMSC grew by static adherence, differentiated to osteoblasts and had strong capacity of proliferation, the shape of cells was fusiform or polygon, like osteoblasts.
鸡囊胚细胞体外培养时,添加10%的胎牛血清,细胞的贴壁率最高;
When culturing blastodermal cells of chicken in vitro, the cell attachment rate is maximum with 10% fetal calf serum.
而且细胞的贴壁效果也是观测分析的关键条件之一。
The effect of cellular adherent was a major condition for analysis.
结果表明:各组细胞正常贴壁,生长良好,细胞形态正常。
The results showed: each group of cells grown well and the form of cell was normal.
对贴壁细胞的生长及增殖活性进行观察;
Then growth and proliferation of the cells were observed from the micrograph.
人胎盘胶原提高了气管上皮细胞的贴壁性。
Coating human placenta collagen enhanced attachment of epithelial cells.
观察不同分离条件对表皮细胞生长情况、贴壁率、克隆形成率的影响。
The output of viable cells, rates of adherence to dish, colony forming efficiency(CFE) of the isolated epidermal ce.
非贴壁层细胞形成CFU-GM的能力达6周以上,但随着培养时间的延长,CFU-GM的形成活性明显下降。
The non-adherent cells of the cultures could generate CFU-GM for more than 6 weeks With a rapid decline in it's output as the cultures going on.
结论:人脐血CD34+细胞群中存在造血基质细胞的前体细胞,人脐血贴壁细胞具有造血基质细胞的某些生物学特点。
It is concluded there are hematopoitic adherent precursors in cord blood CD34+ cells and the HUCBAC shows some biological behavior of hematopoitic stromal cells.
结果丝素膜能支持细胞在其表面贴壁,生长曲线和染色体正常。
Results Bone marrow stem cells can attach and grow on silk fibroin films; growth curve and chromosome were normal.
结论视网膜细胞混合培养有利于神经节细胞的贴壁和生长。
Culturing retinal cells in commixture benefits the paste and growth of ganglion cells.
转化细胞则不大贴壁、圆缩并呈杂乱分布。
Transformed cells are loosely attached, rounded and randomly oriented.
结果:高能X线照射后,贴壁细胞减少。
RESULTS: Fewer adenoid cystic carcinoma cells survived after radiation of high energy X-ray.
体外培养以获得贴壁细胞。
无菌条件下取正常足月剖腹产的脐带近胎儿段,剔除动静脉,取出其中的间质组织,胶原酶消化法原代培养细胞进行培养和纯化获得贴壁细胞。
Artery and vein were removed and mesenchymal tissue was taken out. The primary cells were performed culture and purification with collagenase digestion method to obtain the adherent cells.
第3天贴壁的细胞球继续增殖(约50个细胞大小),漂浮的细胞则增殖成4~8个大小的细胞球;
At 3 days, the adhesive cell balls continued to proliferate(about 50 cells), and the floating cells proliferated into cell balls(4 to 8 cells).
复苏后分离所得胎肝细胞用锥虫兰染色评估细胞活率,并记录贴壁时间,观察集落生长情况。
The survival rate of thawed cells was evaluated by trypan blue staining, and the adherence time and colony growth were observed.
复苏后分离所得胎肝细胞用锥虫兰染色评估细胞活率,并记录贴壁时间,观察集落生长情况。
The survival rate of thawed cells was evaluated by trypan blue staining, and the adherence time and colony growth were observed.
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