• 实现了快速制备组织细胞裂解液

    Thus, quick preparation of the histiocyte lysate can be realized.

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  • 血红细胞裂解

    Whole blood red cell lysing reagent;

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  • 细胞裂解加入蛋白酶抑制剂后可以保护细胞提取物中的蛋白

    The addition of protease inhibitors to the cell lysis buffer AIDS in the preservation of target proteins in the cell extract.

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  • 细胞裂解加入磷酸酶抑制剂后可以保护细胞裂解时磷酸化基。

    The addition of phosphatase inhibitors to the cell lysis buffer AIDS in the preservation of phosphorylated residues at the time of cell disruption.

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  • 结果经过5筛选细胞裂解液洗脱出噬菌体效价有500以上增长;

    Results 500?fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

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  • 本文首先多次联合消化脂肪组织方法分离培养脂肪细胞通过方法去除细胞减小细胞裂解液细胞损伤

    Human ADSCs were isolated firstly in this study with improved method, through the exchange of the cell medium to remove the red blood cells, reducing the damage of cells.

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  • 免疫激活剂昆布多糖存在下,分别得小菜蛾幼虫细胞碎片细胞裂解血浆的酚氧化酶活性26.80U, 16.68 U和2.53 U。

    When laminarin as an immune activator was present, phenoloxidase (PO) activities in cell debris, hemocyte lysate solution and plasma were measured as 26.80, 16.68, and 2.53 u, respectively.

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  • RIPA缓冲用于裂解细胞组织

    RIPA buffer is used to lyse cells and tissues.

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  • 然后裂解细胞测定细胞抽提中TRAP活性并且测定od 450值。

    Cells were lysed and TRAP activity of cell extracts was assessed and OD450 determined.

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  • 另外选取肿瘤细胞H1299,倍比稀释裂解细胞应用配制好的反应进行ATP检测分析最低检测细胞

    Then standard curve of ATP were drawed. Tumor cell lines H1299 were serial diluted and their ATP were measured by reaction solution prepared to analyse the minimum cell number.

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  • 生长ND6菌株细胞提取存在催化裂解途径的儿茶酚1,2-双加氧酶活性,也存在催化间位裂解途径的儿茶酚2,3-双加氧酶活性。

    When naphthalene was used as a substrate for growth of strain ND6, catechol 1,2-dioxygenase and catechol 2,3-dioxygenase activities were both detected in their crude extract.

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  • 结果结论血小板裂解脐带间充细胞增殖活性诱导分化正面影响,这使血小板裂解液细胞移植治疗中将会起到无可比拟的作用

    RESULTS AND CONCLUSION:Platelet lysate plays a positive role on the proliferation and differentiation of UCMSCs, thus indicating a significant role in the stem cell transplantation.

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  • 结果结论血小板裂解脐带间充细胞增殖活性诱导分化正面影响,这使血小板裂解液细胞移植治疗中将会起到无可比拟的作用

    RESULTS AND CONCLUSION:Platelet lysate plays a positive role on the proliferation and differentiation of UCMSCs, thus indicating a significant role in the stem cell transplantation.

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