实现了快速制备组织细胞裂解液。
Thus, quick preparation of the histiocyte lysate can be realized.
全血红细胞裂解液;
向细胞裂解液中加入蛋白酶抑制剂后可以保护细胞提取物中的靶蛋白。
The addition of protease inhibitors to the cell lysis buffer AIDS in the preservation of target proteins in the cell extract.
向细胞裂解液中加入磷酸酶抑制剂后可以保护细胞裂解时磷酸化残基。
The addition of phosphatase inhibitors to the cell lysis buffer AIDS in the preservation of phosphorylated residues at the time of cell disruption.
结果经过5轮筛选,细胞裂解液中洗脱出噬菌体效价有500倍以上增长;
Results 500?fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.
本文首先用多次联合消化脂肪组织的方法分离培养脂肪干细胞,通过换液的方法去除红细胞,减小了红细胞裂解液对细胞的损伤。
Human ADSCs were isolated firstly in this study with improved method, through the exchange of the cell medium to remove the red blood cells, reducing the damage of cells.
在免疫激活剂昆布多糖存在下,分别测得小菜蛾幼虫血细胞碎片、血细胞裂解液和血浆的酚氧化酶活性为26.80U, 16.68 U和2.53 U。
When laminarin as an immune activator was present, phenoloxidase (PO) activities in cell debris, hemocyte lysate solution and plasma were measured as 26.80, 16.68, and 2.53 u, respectively.
RIPA缓冲液用于裂解细胞和组织。
然后裂解细胞,测定细胞抽提液中TRAP的活性并且测定od 450值。
Cells were lysed and TRAP activity of cell extracts was assessed and OD450 determined.
另外选取肿瘤细胞株H1299,倍比稀释后裂解细胞应用配制好的反应液进行ATP检测,分析最低检测细胞数。
Then standard curve of ATP were drawed. Tumor cell lines H1299 were serial diluted and their ATP were measured by reaction solution prepared to analyse the minimum cell number.
以萘为底物生长时,ND6菌株的细胞提取液中既存在催化邻位裂解途径的儿茶酚1,2-双加氧酶活性,也存在催化间位裂解途径的儿茶酚2,3-双加氧酶活性。
When naphthalene was used as a substrate for growth of strain ND6, catechol 1,2-dioxygenase and catechol 2,3-dioxygenase activities were both detected in their crude extract.
结果与结论:血小板裂解液对脐带间充质干细胞的增殖活性及诱导分化有正面影响,这使血小板裂解液在干细胞移植治疗中将会起到无可比拟的作用。
RESULTS AND CONCLUSION:Platelet lysate plays a positive role on the proliferation and differentiation of UCMSCs, thus indicating a significant role in the stem cell transplantation.
结果与结论:血小板裂解液对脐带间充质干细胞的增殖活性及诱导分化有正面影响,这使血小板裂解液在干细胞移植治疗中将会起到无可比拟的作用。
RESULTS AND CONCLUSION:Platelet lysate plays a positive role on the proliferation and differentiation of UCMSCs, thus indicating a significant role in the stem cell transplantation.
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