茜素红s染色观察钙结节计数并测定细胞钙沉积含量。
Alizarin red s staining was applied to count the calcium nodus, and cell calcium depositions were measured.
茜素红s染色观察钙结节计数并测定细胞钙沉积含量。
Alizarin red s staining was applied to count the calcium nodus and the cell calcium depositions were measured.
主要观察指标：形态观察、增值率测定、碱性磷酸酶染色、细胞基质钙含量测定、茜素红染色显示矿化结节数。
MAIN OUTCOME MEASURES: Morphological observation, determination of proliferation ratio, ALP stain, determination of matrix calcium accumulation and number of mineralized nodes with alizarin red stain.
细胞培养4周后，茜素红s染色法示结节呈红色钙阳性反应。
Alizarin red s staining showed that the reaction of calcifying nodules was positive when cultured for 4 weeks.
通过茜素红（ARS）染色，MTT法及其他组织化学的方法来研究在RCCS中培养14天时力学环境对成骨细胞功能状态的影响及其三维培养的研究的成骨潜能。
The osteogenic potential of the cell-CHA scaffold constructs cultured in RCCS were investigated by Alizarin Red S(ARS) staining, MTT method and histochemistry at 14th .
通过茜素红（ARS）染色，MTT法及其他组织化学的方法来研究在RCCS中培养14天时力学环境对成骨细胞功能状态的影响及其三维培养的研究的成骨潜能。
The osteogenic potential of the cell-CHA scaffold constructs cultured in RCCS were investigated by Alizarin Red S(ARS) staining, MTT method and histochemistry at 14th .
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