方法：采用全细胞膜片钳技术，记录钠通道电流。
Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent.
方法传代得到单个HERG-HEK细胞，应用全细胞膜片钳技术记录HERG-HEK细胞上的HERG钾电流和动力学曲线（激活、失活、复活和去活化）。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
膜片钳技术是一种以记录离子通道的离子电流来反映细胞膜上单一的或多个的离子通道分子活动的技术。
Patch clamp technique is a kind of technique which reflecting membrane molecular activity of one or more ion channel by record ion current of ion channel.
方法全细胞膜片箝技术。
采用全细胞膜片钳技术，记录动作电位和上述各离子流。
We used current-clamp technique to record action potentials, and voltage-clamp technique to record ionic currents.
方法：运用全细胞膜片钳技术记录持续性钠电流，并观察其在缺氧-复氧模型下的变化。
Methods: the whole cell patch clamp technology was used to record this current and study its change under the condition of hypoxia-reoxygenation model.
方法：应用全细胞膜片箝电流箝制技术，对急性分离的大鼠DRG细胞进行电流箝制。
Methods: The isolated neurons of dorsal root ganglion (DRG) were studied using the whole cell patch clamp technique in current clamp mode.
应用全细胞膜片钳制技术，我们首次报道了在兔的心室肌细胞存在一种电压依赖性的非特异性阳离子流（NSCC）。
Using the whole-cell patch-clamp technique, clear evidence was provided for the existence of a hitherto unreported, voltage-dependent nonselective cation current (NSCC) in rabbit ventricular myocytes.
方法：全细胞膜片箝技术。
方法采用全细胞膜片钳技术。
方法采用全细胞膜片钳制技术。
方法全细胞膜片钳技术。
方法实验采用的方法是全细胞膜片钳技术，实验材料为急性分离的牛蛙背根神经节神经元。
Method The experiment was carried out in freshly isolated bullfrog using a whole-cell patch-clamp technique.
方法应用全细胞膜片钳技术记录乌头碱对大鼠心肌细胞动作电位时程的作用，分别给予奎尼丁、维拉·帕米处理比较二者对动作电位时程的影响。
Methods Effects of quinidine and verapamil on the alteration of action potential duration induced by aconitine were studied by use of the whole cell patch clamp techniques in rat ventricular myocytes.
方法采用全细胞膜片钳技术，进行电流钳制。
Methods The whole-cell patch clamp with current clamp was used.
方法应用全细胞膜片钳制技术。
方法用急性酶解法获得单个大鼠心室肌细胞，标准的全细胞膜片钳技术记录钠通道电流。
The whole-cell patch-clamp recording technique was used to record the change of sodium channel current influenced by carvedilol.
方法采用全细胞膜片钳技术。
实验利用全细胞膜片钳技术研究了荧光染料核黄（NY）标记的支配牙髓的痛感觉神经元的电生理特征。
Whole-cell patch clamp technique was used to study the electrophysiological characteristics of nociceptive trigeminal ganglion (TG) neurons labeled by nuclear yellow (NY).
常规酶解法分离成年大鼠心室肌细胞，采用全细胞膜片钳技术观察U50，488H对钠电流的作用。
The change of sodium currents (INa) induced by U50,488H was detected by whole-cell recording mode using patch clamp.
方法采用全细胞膜片钳技术在急性分离的大鼠背根神经节细胞上观察龙血素B对辣椒素诱发的辣椒素受体电流的影响。
Methods In acutely isolated rat DRG neurons, effects of loureirin B on capsaicin-evoked currents were observed using whole-cell patch-clamp technique.
作形态学观察，通过单细胞膜片钳技术记录电活动。
Meynert nucleus was obtained and dissociated to observe their morphology and record electric activity by patch clamp technique.
膜片钳技术是一种以记录离子通道的离子电流来反映细胞膜上单一的或多个的离子通道分子活动的技术。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
方法采用全细胞膜片钳制技术。
本文综述了近年来利用膜片钳技术研究中药对心肌细胞膜钙通道作用的现状，并对未来研究进行展望。
This article reviewed lately research status of Chinese herbal effect on calcium channels of myocardial cell by patch clamp technique and look ahead the future research.
方法酶解法分离豚鼠单个心室肌细胞，应用全细胞膜片钳技术研究白藜芦醇对豚鼠单个心室肌细胞钾电流的影响。
Methods Enzyme digestion was performed to isolate single ventricular myocyte of guinea pig and whole-cell patch-clamp technique was used to record potassium current.
方法用急性酶解法获得单个大鼠心室肌细胞，标准的全细胞膜片钳技术记录钠通道电流。
MethodsAfter rat hippocampal or cerebral cortical neurons were isolated by pronase e, the IA currents were recorded by whole-cell patch clamp technique.
膜片钳技术是一种以记录离子通道的离子电流来反映细胞膜上单一的或多个的离子通道分子活动的技术。
Methods Enzyme digestion was used to isolate single ventricular myocyte and whole cell patch clamp technique was used to record potassium current.
方法：采用全细胞膜片钳技术记录蟾蜍灵激活cne - 2 Z细胞膜电流并分析其电流特征。
METHODS: the technique of whole-cell patch clamp was used to record the chloride currents and to analyze the characteristics of the currents in CNE-2Z cells.
方法：应用听觉脑干诱发电位和全细胞膜片钳制技术。
METHODS: Auditory brainstem responses (ABR) and whole-cell patch clamp techniques were used.
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