研究人员已经研制出一种比绿色荧光蛋白小的蓝色荧光探针，它更容易标记活细胞中肽融合重组蛋白。
Researchers have engineered a blue fluorescent probe that is smaller than GFP and will improve the labeling of peptide-fused recombinant proteins in living cells.
然而，尽管有了充分特定的应用，但如绿色荧光这样较大的荧光蛋白经常会破坏细胞中蛋白的正常活动。
However, while perfectly specific, the large size of fluorescent proteins like GFP often disrupts the natural activity of proteins inside cells.
目的构建携带报告基因增强型绿色荧光蛋白（EGFP）的反转录病毒载体，并且探讨病毒载体对SK-N-SH神经母细胞瘤细胞株的感染效率。
Objective To construct the retroviral(RV) vector with report gene enhanced green fluorescent protein(EGFP) and to explore the gene transfection efficiency of RV on SK-N-SH neuroblastoma cells.
目的通过对人退变髓核细胞的原代培养，了解其生物学特性，并将绿色荧光蛋白基因转染至细胞内。
Objective Human degenerative nucleus pulposus cells were cultured in monolayer, the biological action of the cells was observed.
以不同感染强度的携带绿色荧光蛋白基因重组腺病毒转染诱导后的脂肪细胞。
The adipose cells were transfected with Ad-GFP at the different multiplicity of infection (MOI).
目的稳定培养人胚胎干细胞，并通过慢病毒载体对其进行绿色荧光蛋白标记。
Objective To establish a culture system for human embryonic stem cell and label the hES cells with GFP by lentivirus.
结论采用绿色荧光蛋白为报告基因的真核细胞转染技术中，脂质体法是效率高、安全性大的方法。
ConclusionUsing GFP as a report gene in eukaryotic cell transfection, the lipofectamine method could get a higher transfection efficiency and higher survival rate for cells.
在他的第一个实验，他的个人色彩六个细胞的透明蛔虫秀丽隐杆线虫的帮助下，绿色荧光蛋白。
In one of his first experiments, he coloured six individual cells in the transparent roundworm Caenorhabditis elegans with the aid of GFP.
方法 将绿色荧光蛋白 （GFP）表达质粒导入 膀胱癌细胞株BTT T739，筛选出高表达GFP的细胞克隆并配置成细胞悬液。
Methods BTT-T739 cells, a mouse bladder transitional cell carcinoma cell line, were tranfected with GFP plasmid to screen stable GFP expressing clones.
方法 将绿色荧光蛋白 （GFP）表达质粒导入 膀胱癌细胞株BTT T739，筛选出高表达GFP的细胞克隆并配置成细胞悬液。
Methods BTT-T739 cells, a mouse bladder transitional cell carcinoma cell line, were tranfected with GFP plasmid to screen stable GFP expressing clones.
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