血管平滑肌细胞的原代培养采用贴块法进行。
The primary culture of vascular smooth muscle cell used attachment-block method.
本研究拟建立人嗜铬细胞瘤细胞的原代培养方法。
The study is to establish a method of separating and primarily culturing of human pheochromocytoma cells.
结论：在对根、冠髓细胞的原代培养中，组织块法优于组织块酶解法。
CONCLUSIONS: tissue explant method was superior to tissue explant-collagenase digestion method for the culturing of human dental coronal and root pulp cells.
目的通过对人退变髓核细胞的原代培养，了解其生物学特性，并将绿色荧光蛋白基因转染至细胞内。
Objective Human degenerative nucleus pulposus cells were cultured in monolayer, the biological action of the cells was observed.
目的：研究体外培养乳鼠胸骨剑突软骨细胞的原代培养方法，探讨该方法应用的可行性和应用价值。
Objective: To investigate the method of the primary culture chondrocyte from newborn mouse breastbone , and discuss the feasibility of this method and it's application value.
方法：(1)建立与改良小鼠成骨细胞的原代培养方法，并通过碱性磷酸酶和钙结节染色方法进行细胞来源鉴定。
Methods: (1) We built and improved the method of osteoblasts primary culture in vitro in mice, and identified the origination of osteoblasts by AKP and calcium nodes dyeing.
本论文进行了中国对虾血细胞的原代培养，用WSSV感染培养的细胞，检测了病毒对培养细胞的感染以及在细胞内的增殖。
We primarily cultured haemocytes of Fenneropenaeus chinensis, infected with WSSV, and detected the WSSV and its propagation in cultured cells.
方法：膜片钳全细胞记录测量原代培养大鼠海马神经元膜的钾离子电流。
Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.
迄今为止我们已取得了突变的原代培养的细胞以及蠕虫”。
To date we have made mutant tissue-cultured cells as well as worms.
方法以人脐静脉内皮细胞（HUVEC）原代培养模型，MTT法测定紫杉醇对HUVEC增殖的影响；
Methods Human umbilical vein endothelial cells were cultured primarily in vitro and influence of Paclitaxel on HUVEC proliferation was evaluated by MTT method.
结果：原代培养的RPE细胞含大量色素呈多角形，传代的细胞透明呈梭形。
Results: Primary cultured RPE cells contained large amount of pigments in polygonal shape. Passages of cultured RPE cells presented transparent spindle shape.
目的研究原代培养的脊髓神经干细胞在小鼠视网膜的整合和分化情况。
Objective to study the integration and differentiation of spinal neural stem cells after transplantation into mice retina.
研究芳烃类有机溶剂对原代培养大鼠神经胶质细胞的谷氨酸重摄取的影响。
To study effect of aromatic hydrocarbon solvents on the reuptake of amino acid by primary cultured glia cells.
结论：酶消化法原代培养牛视网膜周细胞是一种稳定、可靠的方法。
Conclusion The enzyme digestion procedure is a stable and reliable method to obtain bovine retinal pericytes.
目的：探索原代培养大鼠肝细胞的最佳分离方法。
Aim: To explore the best isolation methods of rat liver cells from primary culture.
结论1、用原代培养的方法培养肾上腺细胞可获成功。
Conclusion: 1, the adrenal cells can be cultured by the methods of primary successfully.
目的以原代培养小鼠骨髓基质细胞为饲养层，探讨精原干细胞能否在该饲养层上生长及生长的影响因素。
To study whether spermatogonial stem cells can grow on the feeder layers of bone marrow stromal cells, and their effecting factors in vitro.
为了更为有效地利用该模型小鼠，我们通过原代培养建立了该模型的肿瘤细胞系。
We also established the cell line of this transgenic mice model through primary cell culture to make use this model more efficiently.
目的：建立大鼠耳蜗感觉上皮细胞(CSEC)的原代培养体系，为研究毛细胞再生的分子机制提供大量来源一致的细胞。
Objective: To further investigate the mechanisms of hair cell generation or regeneration, the primary culture systems of cochlear sensory epithelial cell (CSEC) of rats were established.
目的研究溶血磷脂酸(LPA)对原代培养大鼠小脑颗粒细胞的细胞毒性作用及其损伤机制。
Objective to study the injurious mechanism and the cytotoxic effect of lysophosphatidic acid (LPA) on primary rat cerebellar granule cells.
目的对大肠腺瘤细胞原代培养的方法作进一步的改进。
Objective To improve the primary cell culture methods of colorectal adenomas.
结果流式细胞术证明，MSC具有间充质细胞的特征。原代及传代培养显示，胎儿骨髓msc具有活跃增殖的能力。
Results Flow cytometry analysis showed that fetal bone marrow derived MSC exhibited the characteristics of mesenchymal cells, MSC had an active proliferative ability in primary and passage cultures.
目的：建立快速、有效的绒毛滋养细胞体外原代培养的方法以及建立人滋养细胞株和初步评价。
Objective:To establish the quick and valid primary culture method of human placental villus in vitro and set up a human trophoblastic cell line with preliminary assessment.
用组织块直接培养法，也可以得到皮肤的原代培养细胞。
The primary culture cells of rabbit skin tissue could be obtained by explant culture too.
目的探讨体外原代培养大鼠肝细胞的分离方法。
ObjectiveTo explore the isolating methods of rat hepatocytes for primary culture.
结果原代培养的细胞ALP染色阳性区域可以达90%以上，传代培养后细胞阳性率为100%。
Results the area positive for ALP staining is no less than 90% in the primary culture and almost 100% in the subculture.
目的：建立一种简单高效的中脑多巴胺神经元细胞原代培养方法，并观察胰酶消化对中脑多巴胺能神经元突起生长的损伤作用。
Objective: To establish an easy and high-yield method of rat dopaminergic neuron primary culture, and to observe the damage to the neurites by the trypsogen.
目的探讨糖皮质激素对原代培养老鼠脂肪细胞的糖转运活动及胰岛素信号肽的影响。
Objective To explore the effect of glucocorticoid on glucose transport activity and the expression of insulin signaling peptides in the primary cultured rat adipocytes.
观察柴胡有效成分柴胡皂甙对原代培养贮脂细胞（FSC）激活及合成细胞外基质（ECM）的作用。
Objective: to observe the effects of Saikosaponin, an effective component of Bupleurum, on activating the primary cultured Fat Store Cells (FSC) and synthesizing extracellular matrix (ECM).
方法：通过建立新生大鼠皮层神经元原代培养，用不同浓度醋酸铅染毒和给予牛磺酸干预，观察神经细胞的生长和存活情况。
Methods:Put taurine and different concentrations of lead acetate in cultures of cortex neurons and observe the length of dendites and the growth activity of neurons.
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