体细胞电融合技术因其融合效率高、对细胞无毒害作用而愈来愈普遍地用于细胞工程技术操作中。
Electric fusion of somatic cells has been more widely used recently in cell engineering manipulation because of its high fusion frequency and non-toxic action to the cells.
采用微加工技术将芯片的细胞电融合小室制作成微电极阵列以实现细胞的大量融合，提高细胞的融合效率。
The cell fusion room of chip was completed by MEMS and made into an array of the microelectrode to realize huge cell fusion and to improve the efficiency of cell fusion.
目的：探讨胞质内直接注核法和电融合法对大鼠-小鼠种间体细胞核移植重组胚发育的影响。
Objective:To study the effect of reconstructed embryos by cytoplasm injection and electrical fusion in rat-mouse inter-species somatic cell nuclear transplantation.
结论优化的电融合参数是决定供体核-卵母细胞复合体融合与重组胚激活的关键因素。
Conclusion Optimal parameters for electrofusion are crucial for the fusion of donor-recipient pairs and the activation of the reconstructed embryo.
结果电融合与磁性分选技术相结合可获得95%的融合细胞纯度。
Results The higher cell fusion efficiency can be obtained with electroporator and about 95% purity of fusion cells achieved by magnetic cells sorting unit.
结果电融合与磁性分选技术相结合可获得95%的融合细胞纯度。
Results The higher cell fusion efficiency can be obtained with electroporator and about 95% purity of fusion cells achieved by magnetic cells sorting unit.
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