方法:细胞分离培养于椎板和髂嵴的松质骨,镜下计数细胞数目和台盼蓝染色检测细胞活力。
Methods. Cells obtained from cancellous bone of both vertebral lamina and iliac crest were cultured and proliferation was evaluated by direct cell counting and viability detected by Trypan blue.
方法:对30例大白鼠进行左侧单肺移植,利用透射电镜和病理学技术,检测移植肺、健侧肺及空白对照肺组织胀亡细胞,MDA及SOD活力。
Methods: Pulmonary transplantation was carried out with 30 rats. Cell oncosis, MDA, and SOD activity were detected by using transmission electron microscope and pathological techniques.
对玻璃化冻存的胚脑细胞结构与功能进行检测,分析影响冻存后胚脑细胞活力的因素。
The structures and functions of ECC after cryopreservation were examined, and the factors affecting the viability of ECC were analyzed.
免疫组化方法观察电针组星形胶质细胞BDNF的表达,MTT法检测电针血清对星形胶质细胞活力的影响。
Immunohistochemical method was introduced to observe the BDNF expression of astrocytes in electrical needle group, MTT method to examine the effect of serum on the astrocytes activity.
用外周血淋巴细胞ACP活力检测作为淋巴细胞溶酶体膜通透性改变的标志。
Estimation of lymphocyte acid phosphatase (ACP) activity was adopted as the marker of the change of lysosomal membrane permeability.
以强化训练的武术运动员为观察对象,检测实验前后红细胞中超氧化物歧化酶(SOD)活力,丙二醛(MDA)含量。
The Wushu athletes during their intensive training were observed, and the SOD activities and MDA contents of erythrocyte were tested.
用乳酸脱氢酶(LDH)的释放和四唑盐比色法(MTT法)评估细胞活力及检测细胞增殖。
The viability of HPMC was assessed by lactate dehydrogenase (LDH) release and tetrazolium salt colorimetry assay (MTT assay); MTT assay was also used to assess the growth of the cells.
检测模型动物外周血常规、胸腺指数和脾脏指数、红细胞膜atp酶活力变化。
Changes of orbit blood routine, thymus index, spleen index and ATPase activity of red cell membrane of model mice were tested.
台盼蓝染色作为细胞活力指标,用显微镜检,NBT还原实验和墨汁吞噬从形态、功能两方面检测细胞分化情况。
We take Trypan blue stain as the index of cell activity and do morphological and functional examination by NBT-reducing and ink- phagocytosing.
方法:采用体外培养,MTT法检测维持性血液透析患者血清对细胞相对活力的影响。
METHODS: We adopt the outside development of HUVEC, and use the MTT method to investigate the influence of the maintenance hemodialysis patients' serum to HUVEC.
方法:采用体外培养,MTT法检测维持性血液透析患者血清对细胞相对活力的影响。
METHODS: We adopt the outside development of HUVEC, and use the MTT method to investigate the influence of the maintenance hemodialysis patients' serum to HUVEC.
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