利用胰酶-乙二胺四乙酸、核酸酶处理猪心瓣膜制备脱细胞支架。
Decellularized porcine heart valves were prepared by using Trypsin-ethylenediamine tetraacetic acid and DNase.
细胞支架复合组材料表面及孔隙内均有细胞附着。
There were cells adherent to the surface of materials as well as the inside of ventage in the cytoskeleton compound group.
结果：脱细胞支架细胞成分完全去除，胞外基质保存完好；
RESULTS: The cellular components of heart valves were completely removed and the structure of extracellular matrix was well reserved.
背景：应用固态载体作为细胞支架，修复关节软骨缺损，已有成功经验。
BACKGROUND: It has been successful to repair articular cartilage defects by using solid carrier as cytoskeleton.
细胞、生物材料制备的细胞支架，以及组织和器官的构建是组织工程的三大要素。
Cell, biomaterial prepared cells scaffold and formation of tissues and organs are three main factors for the tissue engineering.
目的观察微重力旋转培养系统对细胞种植于支架和细胞支架复合物体外培养的影响。
Objective to investigate the effects of RCCS on cell seeding onto 3-d scaffold and cell-scaffold composite culture in vitro.
目的验证自行制备的温敏性壳聚糖水凝胶作为软骨细胞支架材料构建组织工程化软骨的可行性。
Objective to reconstruct tissue engineered cartilage using temperature responsive chitosan hydrogel, which were prepared as scaffold materials.
英国研究者将小片干细胞支架注射到中风受损的鼠脑内，结果发现受损的鼠脑内长出了新的组织。
Researchers in the UK inserted tiny scaffolds with stem cells attached into the stroke damaged brains of rats and found that they grew into new tissue to fill the holes made by the stroke damage.
曾经有其他的研究在尝试应用干细胞支架长满填充受损的鼠脑，但是发现这些支架迁移到了其他组织。
Other studies have tried to fill holes left by stroke damage with stem cells but found they just migrated to surrounding tissue.
结论具有类骨磷灰石涂层的PLGA具有更高的细胞亲和性，可望成为一类新的骨组织工程细胞支架材料。
Conclusion PLGA with a bone-like apatite coating has a good cell affinity. It is a hopeful new scaffold material to be used in bone tissue engineering.
观察关节软骨细胞在几丁糖凝胶中培养的生物学行为，以探讨几丁糖凝胶作为组织工程细胞支架的可行性。
Objective To observe the biological behavior of chondrocyte in chitosan gels in vitro and the possibility of chitosan using as scaffolds in tissue engineering.
结论PLLA—PD XB复合材料具有优良的细胞亲和性，可望成为一类新的骨组织工程细胞支架材料。
Conclusion the PLLA-PDXB composite has a good cell affinity, and this kind of composite can hopefully become a new scaffold material to be used in the bone tissue engineering.
目的：应用纤维蛋白胶作为细胞支架进行兔骨髓基质细胞立体培养，探讨其作为新型骨组织工程支架材料的可行性。
AIM: to observe three-dimensional culture of rabbit marrow stromal cells (MSCs) in fibrin glue in vitro, and discuss the feasibility of the fibrin glue to be use as a kind of scaffold material.
理想的细胞支架应具有一定的生物降解速度、良好的细胞亲和性、呈特定的三维多孔结构，且含有可控制释放的生长因子。
For an ideal cells scaffold, it has to possess certain biodegradation rate, good cells affinity, three dimension of multi- porous structure, as well as included controllable released growth factor.
方法多聚赖氨酸包埋PLA，PGA，PLGA三维细胞支架。分离培养兔关节软骨细胞，体外扩增后种植到三种支架中。
Methods Coated PLA, PGA, PLGA with poly-l-lysine, free chondrocytes isolated from rabbit articular cartilage were seeded onto three kinds of scaffolds after expansion by in vitro culture.
生物人工肝反应器中肝细胞的数量和功能是能否有效替代已衰竭肝脏功能的关键，与此紧密相关的是细胞支架材料结构和性能的优化。
The numbers and the function of the hepatocytes in the bioartificial liver device is the key point determining the efficiency of the substitution of the crocked up liver.
结果实际完成观察的关节数为44个，其中空白对照组9个，脱细胞软骨支架组11个，静态培养细胞支架组18个，生物反应器培养细胞支架组6个。
Results Observation was completed only for 44 joints, with 9 ones in the blank group, 11 in the simple scaffold group, 18 ones in the static culture group, and 6 ones in the bioreactor culture group.
结果表明，两种支架都具有良好的细胞相容性。
Results showed that both the scaffolds have good cyto-compatibility.
之后包括模板和细胞立方体在内都要用聚乙二醇再次包被，后者作为粘合剂使立方体块紧密相连并牢牢地固定在支架表面。
Both template and cell cubes are coated again with the PEG polymer, which ACTS as a glue that holds the cubes together as they pack themselves tightly onto the scaffold surface.
两天后，他们把泌尿细胞涂在支架里面。
Two days later, they coat the inside of the scaffold with urothelial cells.
所得物保持原来支架的长度和直径，由胶原蛋白和平滑肌细胞组成，其结构类似天然血管。
The result is a tube of the length and diameter of the original scaffold, that is composed of collagen and smooth-muscle cells—a structure similar to a natural blood vessel.
丝类支架可以被用作组织工程学领域的细胞基质，它能提供细胞生长所需的立体环境。
Silk scaffolds are used as a cell substrate in tissue engineering, providing a three-dimensional environment in which cells can grow.
捐赠者的细胞会从气管上剥去，而只剩下软骨支架，随后在其上植入从受移植者者骨髓中采集到的干细胞。
The donated tracheas were stripped of the donors' cells — leaving only the cartilage scaffold — and then reseeded with stem cells derived from the recipients' own bone marrow.
这具支架和生物反应堆随后被海运到斯德哥尔摩，并在此处加入了含有患者干细胞的溶液。
The scaffold and bioreactor were then shipped to Stockholm, where the solution containing the patient's stem cells was added.
尽管打印器官这一技术还处于初级阶段，但是在支架中培育细胞生长已经取得成功。
Though printing organs is new, growing them from scratch on scaffolds has already been done successfully.
目前，这位匿名的男孩依然留在医院，医生需要监视气管上干细胞的生长情况，气管有望在两周内完全生长成型，在此期间，气管被支撑在一个人工支架上，等成型后支架会自溶掉。
The boy, who has not been identified, will remain in hospital while the growth of the cells is monitored. It is expected that the windpipe will be fully formed within two weeks.
两天时间干细胞长满了支架的表面和内测。一个生物学上Beyene自己的组织器官就此诞生。
The stem cells took hold and within just two days had filled the scaffold, creating a new trachea that is, biologically speaking, Beyene’s own tissue.
通过模拟血管壁平滑肌细胞周围基质，这种支架可用于修复血管损伤。
Such scaffolds might be used to repair damaged arteries by mimicking the natural matrices that surround smooth muscle cells in vessel walls.
通过模拟血管壁平滑肌细胞周围基质，这种支架可用于修复血管损伤。
Such scaffolds might be used to repair damaged arteries by mimicking the natural matrices that surround smooth muscle cells in vessel walls.
应用推荐