目的通过对肝细胞与肝癌细胞E钙粘素分布、表达和运动生长行为与其连结状态和骨架预张力状态相关关系的研究，探索肝癌细胞接触抑制丧失机制。
Object Through researching the distribution and expression of E-cadherin, cell movement and cytoskeleton in the liver cells and liver cancer cells, we explore the loss of contact inhibition mechanism.
该细胞贴壁生长，具有接触抑制的性质。
These cells were able to grow adhering to the wall and stop growing with contact inhibition.
细胞呈贴壁生长，趋向复层，无接触抑制。
The cell line grew with adherence, showing a tendency of multi layer growth without contact inhibition.
从转化灶中分离的细胞，失去了在培养中的接触抑制，能在软琼脂培养基中形成集落。
The transformed cells separated from the foci lost their ability of post-confluence inhibition of division, and could grow into colony in soft AGAR medium.
组细胞随培养时间的延长细胞呈多层生长，不发生接触抑制现象。
The cells in the Group a grew with many layers along with the prolongation of the culture time without contacted inhibition.
结论表明生长期的细胞做供体细胞融合率及囊胚率显著高于经过接触抑制处理和血清饥饿处理的细胞。
Through the data we found that growing cells do donor cell fusion rate and blastocyst rate were significantly higher than after treatment and contact inhibition in cells treated with serum starvation.
结论表明生长期的细胞做供体细胞融合率及囊胚率显著高于经过接触抑制处理和血清饥饿处理的细胞。
Through the data we found that growing cells do donor cell fusion rate and blastocyst rate were significantly higher than after treatment and contact inhibition in cells treated with serum starvation.
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