收集细胞悬液,再经低渗处理后滴片染色。
Cell suspension was collected, and then stained after hypotonic treatment.
在心肌梗死后瘢痕组织中移入干细胞悬液。
Myocardial infarction transplantation group Stem cell suspension was injected into the scar tissue after myocardial infarction.
所回收细胞悬液中活细胞量用染料排除试验超过90%。
The amount of viable cells present in the recovered cell suspension exceeds 90% as examined by dye exclusion test.
把软骨细胞悬液直接滴加到PGA支架上进行复合培养。
The chondrocytes were seeded on the PGA scaffolds directly in control group.
本实验比较了胎脑组织块和细胞悬液脑实质内移植两种方法。
The fetal brain tissue piece and cell suspension graft were compared and the results showed that the method of the cell suspension was better than of tissue piece.
目的分析重症缺铁性贫血患儿输注红细胞悬液或全血的临床疗效。
Objective to analyse clinical curative effect to the advanced case of childhood IDA in the red cell suspension liquid or whole blood.
结论模拟临床钻孔减压手术向兔股骨头内注入细胞悬液是可行的。
Conclusion femoral head osteonecrosis can be treated with trephination decompression of femoral head and infusion of cell suspension into femoral head of rabbits.
骨骼肌在制成单细胞悬液过程中的人为损伤,可造成DNA提前降解。
The artificial damage of the skeletal muscle during the preparation of single cell suspension could cause the earlier degradation of the DNA.
将细胞悬液滴入预先制好的生物骨衍生材料粗糙面上,使之充分渗入。
The cells were dripped and filtered into the derived material's coarse faces.
目的:探索全胚和胎盘组织细胞悬液及其间充质干细胞的抗衰老作用。
Objectives: To explore the anti-aging effect of the cells suspension and mesenchymal stem cells derived from murine fetus and placenta.
本文介绍了胎肝细胞悬液的制备方法,此法简便、成本低廉、使用安全。
This paper introduces a method of preparing fetal liver cell suspension, it is simple and convenient, much cheaper and safer.
通过对骨微管结构的细胞及细胞悬液的两相流分析,得出了结构中细胞浓度的分布。
Two-phase flow analysis in the bone microtubules is analyzed and the distribution of cell volume concentration is demonstrated.
方法用S180腹水型肉瘤小鼠的腹水肿瘤细胞悬液接种小鼠左腋皮下复制肿瘤模型。
Methods S180 ascites sarcoma mice models were made by transplanting the ascites sarcoma cell suspension in the mice left anterior subcutaneous part.
用妊娠4 ~5月胎肝制成新鲜胎肝细胞悬液输注,治疗了再障和化疗后的白血病。
Freshly prepared fetal liver cell suspension derived from the foetuses of 4-5 months of gestation were transfused to a group of patients with aplastic anaemia and leukemia after chemotherapy.
以18天鸡胚睾丸为实验材料,经胶原酶和胰蛋白酶两步酶消化法得到生精上皮细胞悬液。
Testes from 18-day-old embryonic chickens were used to isolate seminiferous epithelial cells by sequential two-step enzyme digestion with collagenase and trypsin.
方法取大鼠胚胎分离成纤维细胞,分别采用细胞悬液法和胰酶消化植块培养法对其进行培养。
Methods Separate the embryonic fibroblast of mice and culture with the method of suspension culture and method of trypsinization.
方法:实验采用干细胞分离液,利用梯度密度法分离患者自体骨髓干细胞,制备干细胞悬液。
METHODS: Autologous BMSCs were isolated by density gradient centrifugation with stem cell extract liquid to prepare stem cell suspension, and were intramuscularly injected into the suffered limbs.
将心肌细胞悬液分为6组,分别为常氧及缺氧条件下的对照组、葛根素注射液组、硝苯地平组。
The myocardial cell suspension was divided into 6 groups: control group, puerarin injection group, nifedipine group under normal oxygen and hypoxia respectively.
无菌采集正常分娩或剖宫产胎儿脐带血,分离干细胞,经肝动脉介入将干细胞悬液定植入肝脏。
The therapeutic stem cells were collected and isolated from the umbilical blood of fetal delivered by normal or uterine-incision woman.
程序降温法:以甘油和二甲基亚砜为低温保护剂,分别以不同的比例添加到含有细胞悬液的冻存管中。
Programmed cooling method: The glycerol and Dimethylsulfoxide, as cryoprotective agents, were mixed with COC1 cells suspensions at different concentration, respectively.
但是,保存前白细胞滤除更有利于红细胞悬液的保存和输注安全,更适合临床尤其是白血病患者使用。
But it is beneficial to RBC preservation and transfusion safety, and more suitable for clinical use especially patient who suffer from leukemia.
库存两周的红细胞悬液中也可发现细胞碎片及少量微聚体,其红细胞形态正常率与清洗前的回收血相近。
But some particles and even a neurocyte bigger than erythrocyte still could be found in the washed blood.
方法:采用流式细胞术对30例新鲜肺癌组织和5例正常对照组组织制成的单细胞悬液进行了DNA含量分析。
Methods:We analyzed the single-cell suspensions of 30 fresh lung carcinoma tissues and 5 normal tissues of control group to get their DNA levels by using flow cytometry.
将人成纤维细胞制成细胞悬液,分别采用静态接种、转瓶接种和灌注接种方式将细胞接入三维胶原-壳聚糖海绵。
In the present work, human fibroblasts were seeded to collagen-chitosan sponges by static seeding, stirred seeding and perfusion seeding.
结论消化振荡法对人胚胎神经干细胞的活性影响较小,是一种有效、安全的将神经球打散成单细胞悬液的实验方法。
Conclusion This study suggests a valid and safe method that can be used for efficient passage of neural stem cells.
脾脏放置在含有细胞培养用的20毫升10%胎牛血清培养皿里,剪成两瓣,用无菌玻璃的边缘挤压,获得单细胞悬液。
Place the spleen in a Petri plate with 20 ml of RPMI 10% FBS, cut in two pieces and squeeze each of them between two sterile glass slides to obtain a single cell suspension.
自1982年以来,我们为8例慢性再生障碍性贫血孕妇(妊娠4~6月),引产后作自身胎肝细胞悬液转输(FLT)。
Since 1982, 8 pregnant women(in pregnancy of 4~6 months)suffering from chronic aplastic anemia have been treated with auto-FLT after induced abortion.
C57BL小鼠10只,注射B16单细胞悬液于小鼠腹腔(腹腔组)和左后肢(后肢组),构建小鼠黑色素瘤生物力学模型;
The biomechanical animal model was built by injecting the B16F10 melanoma cell suspension into the abdominal cavity and left skeletal muscles in 10 C57BL mice.
结果蚕丝三维支架上滴加软骨细胞悬液后,软骨细胞在不规则轻微漂动和缓慢下沉过程中粘附在蚕丝上,1~2天后完全贴壁。
Results The chondrocytes were adhered to silks slowly after chondrocytes were seeded into silk scaffolds and cells fixed on silks well 1 or 2 days later.
本文对食管癌组织、脱落细胞和血液标本的悬液制备方法作了初步探讨,结果表明,胶原酶对实体肿瘤组织单细胞悬液制备是可行的。
In this report, the method for preparing mono-cellular suspensions from solid mass of esophagus carcinoma, exfoliative cells and blood sample was described.
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