本发明涉及细胞培养技术领域。
The invention concerns the field of cell culture technology.
方法：采用细胞培养技术和组织工程学方法。
METHODS: Cell culture technique and tissue engineering method were used.
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方法：采用标准酶分离技术和细胞培养技术分离培养兔内皮细胞。
METHODS: Rabbit endothelial cells were cultured by standard enzyme separation technique and cell culture technique.
方法利用体外细胞培养技术，通过MTT测定、细胞计数等方法。
Methods To observe the protecfive effecf of TF by measuring MTT metablism and cell numbers and cell culturing in vitro.
方法：应用细胞培养技术、噻唑盐比色测定(MTT)和酶动力学方法。
Methods: Using cell culture technique, MTT and enzyme kinetics methods.
本文综述了十多年来通过固定植物细胞培养技术生产天然产物的研究概况。
This article reviews the advances in the production of natural products by immobilized plant cell cultures.
野生资源的合理利用、红豆杉的人工种植和细胞培养技术是目前主要的解决途径。
To solve this problem, the principal approaches are reasonable utilization of natural resources, artificial growing of Taxus and innovation of tissue culture technology.
方法采用体外细胞培养技术进行皮肤成纤维细胞的分离、培养、传代、冻存、复苏及鉴定。
Methods Cell culture in vitro technique was used, including isolation, culture, subculture, frozen storage, recovery and identification of human fibroblasts, in this study.
方法应用体外细胞培养技术观察MC3T3 - E1成骨细胞株在不同处理的牙骨质表面的生长状况。
Methods Cell culture technique in vitro had been used to study the growth condition of MC3T3-E1 osteoblast line on the surface of cementum treated with various methods.
同时对基因重组技术、细胞培养技术在白藜芦醇制备方面的应用作了系统阐述，并对其应用前景进行了评价。
Meanwhile, the applications of gene recombination technology and cell culture technology in preparation of resveratrol were explained systematically, and its application prospect was also viewed.
本研究为进一步利用细胞培养技术生产甜菜红素以及研究甜菜红素在细胞内的生物合成调节机制奠定了基础。
These results laid the foundation for further producing betacyanin by plant cell culture and studying betacyanin biosynthesis regulation mechanism in plant cell.
方法采用细胞培养技术、免疫细胞化学方法观察新生大鼠皮层神经干细胞向胆碱能神经元定向分化的时空变化。
Observation the space-time change of cortex nerve stem cell in newborn rat orientation differentiation to cholinergic neuron by cell cultured and immunocyte chemistry methods.
三维细胞培养技术应用在生物医学、临床应用和生物制药等领域有重要意义，并讨论与其相关技术装备的进展和应用。
Three-dimensional cell culture has been very significant in the fields of biotechnology, clinical medicine. This article is to present the recent characteristics and applications of these instruments.
方法应用细胞培养技术，测定加入细胞因子及地塞米松后细胞间粘附分子1（ICAM 1）表达和DNA合成情况。
Methods ICAM 1 expression and DNA synthesis in cultured retroocular fibroblasts incubated with cytokines and dexamethasone were measured.
医学院校研究生在课题研究阶段普遍要用到细胞培养技术，所以在基础学习阶段有必要针对性地开展细胞培养实验课。
The technology of cell culture is very useful to graduate students in medical university, so it necessary to apply the cell culture experiment courses to them.
紫杉醇生物合成调控是实现红豆杉细胞培养生产紫杉醇的关键技术之一。
The regulation of taxol biosynthesis is one of the key techniques to produce taxol by cell culture.
另外一种较为复杂的技术，则是把胚胎细胞培养成各式各样的细胞种类及组织。
Another, more complex, technique takes embryo cells and develops them into a wide variety of cell types and tissues.
采用外周血淋巴细胞培养及染色体制片技术，对延边黄牛及其利延杂交牛染色体核型和C带进行了研究。
The chromosome caryogram and C-banding of Yanbian cattle and Liyan hybrid cattle were researched by the culture of peripheral hemolymph cell and chromosomal banding technique.
方法应用细胞培养、疫组织化学、荧光标记、光逆行追踪和酶组织化学等技术。
Methods Cell culture, immunohistochemistry, nucleus fluorescence labeling, fluorescence retrograde labeling and enzymatic histochemistry technique were used.
方法应用细胞培养、免疫组织化学、核荧光标记、荧光逆行追踪和酶组织化学等技术。
Methods Cell culture, immunohistochemistry, nucleus fluorescence labeling, fluorescence retrograde labeling and enzymatic histochemistry technique were used.
方法采用细胞培养染色体畸变技术。
体外肝细胞培养是生物制药和人工肝支持系统的技术基础。
Cultured cells are the basic element of biologic pharmacy and artificial liver system.
目的和方法：利用细胞培养和RT P CR技术研究新生大鼠心脏成纤维细胞增加心肌细胞搏动频率的作用。
Aim and Methods: To study the effect of ventricular fibroblasts increase beating rate on cultured neonatal rat cardiomyocytes by using cell culture and RTPCR technique.
采用外周血淋巴细胞培养及染色体分带技术，分析了海门白山羊的染色体核型与C-带。
The karyotype and C banding of Haimen White goat breed were investigated with the peripheral blood lymphocyte culture and chromosome banding techniques.
采用外周血淋巴细胞培养方法和常规染色体制备技术，对内蒙古乌审马染色体核型进行了分析研究。
In this paper, chromosomal karyotype of Wushen horse in Inner Mongolia was studied by using the peripheral blood lymphocytes culture method and conventional chromosome analysis techniques.
而血清处理和细胞冻存过程中的技术细节若处理不当，会对细胞培养的效果产生较明显的影响。
But, serum handles and produces if cell frozen technology detail in storing process being not handled properly, being going to be to the cell cultivation effect more obvious effect.
而血清处理和细胞冻存过程中的技术细节若处理不当，会对细胞培养的效果产生较明显的影响。
But, serum handles and produces if cell frozen technology detail in storing process being not handled properly, being going to be to the cell cultivation effect more obvious effect.
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