与人骨髓间充质干细胞培养上清共孵育。流式细胞仪检测表面抗原。
The monocytes were co-incubated with supernatant from human marrow(mesenchymal) stem cells culture, and flow cytometer was used to detect surface antigen.
制备免疫耐受大鼠脾淋巴细胞培养上清，用夹心elisa法测定其中IL 10的水平。
The culture supernatants of splenic lymphocytes from oral immune tolerance rats were prepared. The IL-10 level in the supernatants was detected by sandwich ELISA.
在细胞培养上清和小鼠腹水抗体的检测中，其灵敏度比经典的醛化致敏血球提高4～8倍。
The sensitivity of the test was proved to be 4—8 times as sensitive as that of classical passive hemagglutination test.
在细胞培养上清和小鼠腹水抗体的检测中，其灵敏度比经典的醛化致敏血球提高4～8倍。
The sensitivity of the test was proved to be 4—8 times as sensitive as that of classical pa…
结论：推测梅花鹿脾细胞培养上清具有生长因子样活性，并在细胞培养基中代替血清的作用。
Conclusion: it is identified that the splenocytes culture supernatant has growth factor like action and can substitute for serum in the medium.
方法：采用经具核梭杆菌LPS刺激的单核细胞培养上清作用于体外培养的人牙髓细胞，通过OD值测定，观察单核细胞培养上清对人牙髓成纤维细胞碱性磷酸酶（ALP）活性的影响。
Methods:The culture supernatant of monocytes stimulated with Fn LPS was applied to human dental pulp cell in vitro, and activity of ALP in human dental pulp fibroblast was monitored by OD test.
方法：采用经具核梭杆菌LPS刺激的单核细胞培养上清作用于体外培养的人牙髓细胞，通过OD值测定，观察单核细胞培养上清对人牙髓成纤维细胞碱性磷酸酶（ALP）活性的影响。
Methods:The culture supernatant of monocytes stimulated with Fn LPS was applied to human dental pulp cell in vitro, and activity of ALP in human dental pulp fibroblast was monitored by OD test.
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