同时收集26名正常儿童样本为对照组，进行相同血清细胞因子检测。
The samples of 26 normal children were also collected as control group for the same serum cytokines detection.
现就其技术原理、特点及其在泪液细胞因子检测中的应用作简要介绍。
This article briefly reviews the principle and characteristic of liquichip and its application in tear cytokine analysis.
细胞内细胞因子检测的影响因素较多，分析时需注意选择适当的测定条件。
There are many influence factors in the method. It is noted to select the appropriate conditions at the analysis.
《国际肝病》：当检测这些细胞的治疗价值时，患者是否需要注射细胞因子？
Hepatology Digest; Do you have to inject cytokines into humans when testing these cells for therapeutic benefit?
因此，对手术后病人进行动态检测细胞因子，对恶性肿瘤的疗效及预后判断均有指导意义。
It was thought that continual monitoring of cytokines levels in patients with malignant tumor after operation might help to judge the prognosis.
分别应用抗原刺激试验、酶联免疫反应、细胞内细胞因子染色检测上述T细胞的细胞因子表达。
The cytokine profiles of these T cells were detected by antigen stimulated assay, ELISA and intracellular cytokine staining.
目的：探讨ami患者尿激酶治疗后相关细胞因子水平的变化及检测价值。
Objective to explore the changes of the serum levels of related cytokines after urokinase therapy in patients with AMI.
检测CHF大鼠细胞因子的变化，验证CHF的免疫激活理论。
Measure the change of cytokines in CHF rats and verify the theory of immunity activation in CHF.
方法：用流式细胞仪检测30例根治切除的肺癌患者术前、术后不同时期外周血淋巴细胞亚群中各种细胞因子。
Method:The peripheral blood lymphocyte subsets were examined in 30 patients with lung cancer before and after operation by flow cytometric analysis.
本文用半定量RT-PCR方法检测多种细胞因子、细菌抗原、丝裂原对P2X7受体表达的诱导作用，探索P2X7受体的诱导表达模式。
RT-PCR was used to detect the constitutive level of P2X7 receptor and the levels upon stimulation with bacteria, bacterial product, mitogen and various cytokines in human PBMC and monocytes.
生物芯片检测经过归类可列成序列，并可同时对细胞因子和生长因子、孕激素和甲状腺激素、细胞黏附分子以及滥用药物进行检测。
Biochip tests are grouped together as arrays and allow the detection of cytokines and growth factors, fertility and thyroid hormones, cell adhesion molecules, and drugs of abuse.
本文介绍了4种细胞因子的检测方法，并从方法学和应用角度给出了简要评价。
In this article, 4 methods of measurement on cytokine are given and respectively a brief comment is made on the ba-sis of its methodology and application.
方法：采用流式细胞术检测凋亡细胞百分比及基因和细胞因子蛋白含量。
Methods: Flow cytometry was adopted to detect the percentage of apoptosis cells and the expression of gene and cell factor.
检测了细胞倍增时间和IL - 6、趋化因子等细胞因子，从倍增时间和因子产生能力看，各克隆差异很大。
None of the cloned cells were keratin negative. The double time and IL-6 as well as chemokine production varies among different clones.
结论腹腔引流液炎性细胞因子水平检测，可对胆瘘进行早期诊断，避免严重后果。
Conclusions The peritoneal drainage fluid levels of inflammatory cytokines detection can do early diagnosis for biliary fistula and avoid serious consequences.
结果表明，建立的实时荧光定量pcr方法特异性强、重复性好，为定量检测鸡细胞因子的一种快速、准确的方法。
The results showed that the established QRT-PCR was specific and reproducible, and could be used for the rapid and accurate detection of chicken cytokines.
通过检测细胞增殖和细胞因子产生，研究AZM对活化的CD4+细胞的影响。
The effect of AZM on activated CD4+ cells was evaluated with respective cell proliferation and cytokine production.
方法：对酒精性肝硬化代偿期和失代偿期患者及健康者用放射免疫分析法检测细胞因子的含量进行分析。
Methods: The changes in serum cytokine level in compensated and decompensated alcoholic liver cirrhosis patients and healthy controls were measured by radioimmunoassay technique.
目的建立流式细胞术检测细胞内细胞因子的方法。
Objective To develop the technique for measuring intracellular cytokines by3 - colour flow-cytometry.
方法对30例患者经中药治疗前后及26名正常对照者，采用酶联免疫吸附试验（ELISA）检测血清中上述2种细胞因子的含量。
Methods The serum IL-2 and IL-8 levels were determined in 30 patients during acute phase and after disappearance of symptoms and 26 normal controls by enzyme-linked immunosorbent assay(ELISA).
方法对30例患者经中药治疗前后及26名正常对照者，采用酶联免疫吸附试验（ELISA）检测血清中上述2种细胞因子的含量。
Methods The serum IL-2 and IL-8 levels were determined in 30 patients during acute phase and after disappearance of symptoms and 26 normal controls by enzyme-linked immunosorbent assay(ELISA).
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