目的:研究艾灸对HCC癌前病变大鼠细胞周期调控相关基因的影响。
Objective: To study the effect of moxibustion in relative cell cycle control gene change of HCC precancerous lesion on big rats.
目前在分子水平上的研究显示肿瘤的发生与癌基因的激活和抑癌基因的失活以及细胞周期调节失控密切相关。
It has been proved that the occurrence of oncogenesis is in close relationship with the oncogene activation, the tumor suppressor gene inactivation and the disorder of cell cycle modulation.
结论HCMV感染使部分细胞周期相关蛋白基因差异性表达可能是HCMV导致宿主细胞周期紊乱的原因之一。
Conclusions Significantly differential expression of some cell cycle related genes in infected cells could be the mechanism for the cell cycle dysregulation caused by HCMV infection.
这些基因分别与细胞增殖、基因转录、凋亡、信号转导、DNA损伤修复、肿瘤分化和浸润转移及细胞周期调节等相关。
These genes were involved in cell proliferation, gene transcription, apoptosis, signal transduction, DNA damage and repair, tumor differentiation and metastasis, cell cycle, and so on.
免疫细胞化学法检测细胞周期蛋白及凋亡相关基因蛋白的表达。
The expression of cell cycle protein and apoptosis-associated gene protein was detected with immunohistochemical technique.
方法利用免疫细胞化学,蛋白质印迹法,RT -PCR和流式细胞术等方法分析细胞周期和对与细胞周期相关的基因表达进行研究。
Methods the cell cycle and the expression of related genes were analyzed by the methods of immunocytochemistry, protein blotting, RT PCR and flow cytometry.
目的观察dna甲基化和组蛋白乙酰化对人结肠癌细胞系癌相关基因的表达和细胞周期的影响。
Purpose to investigate the effects of DNA methylation and histone acetylation on the expression of tumor associated genes and cell cycle progression in human colonic cancer cells.
结论FTY720在体外可呈时间依赖诱导正常大鼠gmc凋亡,其机制与影响细胞周期调节蛋白及凋亡相关基因的表达有关。
Conclusion FTY720 can induce GMC apoptosis in a time-dependent manner, probably through influencing gene expression of cell cycle regulatory proteins.
表达发生变化的基因主要为与细胞周期和细胞凋亡相关的基因。
The changed genes were mainly associated with cell cycle and apoptosis.
免疫细胞化学法检测细胞周期蛋白及凋亡相关基因蛋白的表达。
The expression of cell cycle protein and apoptosis-associated genes protein was detected with immunohistochemical technique.
我们对部分细胞周期、细胞凋亡、细胞应激与炎症反应相关基因进行了分析并用实时荧光定量pcr进行了验证,证实了基因芯片结果的可靠性。
We analyze some genes including cell cycle, apoptosis, stress and inflammation, which are confirmed by the real time RT-PCR, and the results are corresponded to that of cDNA microarray.
我们以人急性早幼粒白血病细胞株HL-60作为实验对象进行石蒜碱抗白血病作用机制研究,在凋亡相关基因表达变化和细胞周期阻滞两方面展开探讨。
We used human acute promyelocytic leukemia cell line HL-60 to study the anti-tumor mechanisms of lycorine in two aspects: apoptosis and cell cycle arrest.
我们以人急性早幼粒白血病细胞株HL-60作为实验对象进行石蒜碱抗白血病作用机制研究,在凋亡相关基因表达变化和细胞周期阻滞两方面展开探讨。
We used human acute promyelocytic leukemia cell line HL-60 to study the anti-tumor mechanisms of lycorine in two aspects: apoptosis and cell cycle arrest.
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