目的建立一种新的细胞周期特异性凋亡检测方法。
Objective The current study was designed to establish a new method for the analysis of cell cycle specific apoptosis.
细胞周期分析表明，经癌宁处理后，细胞的增殖指数为33.7%，无细胞周期特异性。
Cell cycle analysis showed that the proliferation index was 33.7% after the human gastric adenocarcinoma cells treated with Aining, and there was no cell phase specialty.
结论：“API技术”和亚g 1峰检测法的细胞凋亡域不一致，“API技术”对细胞周期特异性凋亡的检测结果与亚g 1峰检测结果也不一致。
Conclusion: Regions of apoptotic cells in "API method" and sub-G1 method were not the same. There is no coincidence in results of apoptotic percentage detected by "API method" and sub-G1 method.
FCM分析表明该抑制作用是细胞周期非特异性的。
FCM analysis indicates that this inhibiting action is nonspecific cell cycle.
结论载体表达的特异性核酶能有效地抑制HSC的细胞周期蛋白d1的表达和激活。
Conclusion Ribozyme targeting cyclin Dl may effectively inhibit cyclin D1 expression and activation in the HSC T6 cell line.
结论载体表达的特异性核酶能有效地抑制HSC的细胞周期蛋白d1的表达和激活。
Conclusion Ribozyme targeting cyclin Dl may effectively inhibit cyclin D1 expression and activation in the HSC T6 cell line.
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