本研究采用地高辛标记的RNA探针,用原位杂交的方法研究了大鼠胃底腺中胃蛋白酶产生细胞的个体发育。
In this study, the ontogeny of pepsinogen producing cells in rat fundic glands was studied by in situ hybridization using a digoxigenin-labeled RNA probe.
方法组织化学、原位杂交和完整细胞RNA斑点印迹。
Methods Histochemistry, in situ hybridization and intact cell RNA dot blot techniques were used.
肾组织免疫组化染色和原位杂交显示肾小球及肾小管上皮细胞内存在VZV抗原及转录产物rna。
VZV antigen and RNA transcript were found in glomerular and tubular cells by immunohistochemical staining and in situ hybridization of renal tissues, respectively.
利用转化有QHB启动子驱动GUS报告基因的转基因植株和原位杂交,我们发现QHB在根静止中心的中央细胞内特异表达。
Using transformants carrying the QHB promoter-GUS and in situ hybridization, we found that QHB was specifically expressed in the central cells of a quiescent center (QC) of the root.
方法采用常规染色体G 带分析和荧光原位杂交(FISH)方法对155例NHL患者的淋巴结组织进行细胞和分子遗传学研究。
Methods Routine G banding chromosome analysis and fluorescent in situ hybridization(FISH)were performed on lymph node specimens from 155 NHL patients.
方法:淫羊藿甙诱导培养的兔血管平滑肌细胞凋亡,组织原位杂交观察凋亡基因GRP78在兔斑块组织中的表达水平。
Methods: icariin induced cultured rabbit vascular smooth muscle cell apoptosis, in situ hybridization in rabbit plaque GRP78 gene expression levels of the organization.
荧光原位杂交是当前分子细胞遗传学最重要的技术之一,克服了实体瘤经典细胞遗传学研究的主要障碍。
Florescence in situ hybridization (FISH), one of the most important molecular cytogenetic techniques, overcomes the main inconvenience in classical cytogenetic methods in solid tumors.
并可用于流式细胞术、同位素标记、单层细胞超微结构、免疫细胞化学和图像分析、原位杂交等的研究。
These methods were applicable to flow cytometry, isotope label, monolayer cell ultrastructure, immunocytochemistry, image analysis and in situ hybridization as well.
本研究采用KIAA1173基因特异性原位杂交探针,检测其在NPC组织及细胞株中的表达,探讨KIAA1173基因与NPC发病的关系。
This study was to detect the expression of KIAA1173 gene in NPC tissues and cell lines, and investigate its involvement in NPC.
方法用免疫组化和原位杂交研究24例良性肾上腺嗜铬细胞瘤、11例恶性肾上腺嗜铬细胞瘤及11例正常肾上腺组织中vegf的表达情况。
Methods The expression of VEGF in 11 normal adrenal gland, 24 benign adrenal pheochromocytomas, and 11 malignant pheochromocytomas was detected by immunohistochemistry and in situ hybridization.
这9个类型中有的结合细胞学镜检与核酸原位杂交技术已确定为异附加系。
Some of the 9 types have been identified as alien addition lines through cytology techniques and in situ hybridization of nuclear acids.
方法MTT方法观察对照组、RA处理组细胞的增殖变化,原位杂交检测两组细胞中msx基因扩增水平的变化。
Methods To observe the proliferation of the cells by MTT and the expression of MSX gene in the cells between ra treated and control group.
目的建立多色荧光原位杂交技术检测人卵细胞染色体非整倍体的方法。
Objective: To establish the method of aneuploidy detection in human oocytes by multicolor fluorescence in-situ hybridization (M-FISH).
银屑病;成纤维细胞生因子10;原位杂交。
Psoriasis; Fibroblast Growth Factor 10; In situ hybridization.
目的探讨多重荧光原位杂交(M -FISH)技术检测慢性淋巴细胞白血病(CLL)复杂核型异常(CCA)的价值。
Objective To investigate the value of multiplex fluorescence in situ hybridization (M-FISH) in the detection of complex chromosomal abnormalities (CCAs) of chronic lymphocytic leukemia (CLL).
免疫组化和原位杂交阳性信号多位于单核巨噬细胞系统胞浆内。
The positive signals of immunohistochemistry and in situ hybridization were mostly located in cytoplasm of monocyte-macrophage system.
抗病易位系Y96060可通过细胞遗传分析,染色体C分带和原位杂交技术,RAPD技术得到确认。
Said disease-resisting translocation line Y96060 can be confirmed by means of cytogenetic analysis, chromosome C zoning and in situ hybridization and RAPD technique.
方法免疫细胞化学和原位杂交技术。
方法联合应用常规细胞遗传学、间期荧光原位杂交技术和多重荧光原位杂交技术对14例伴有复杂核型异常的aml患者进行研究。
Methods Multiplex FISH was used in combination with conventional cytogenetics (CC) and interphase FISH to study 14 cases of AML with complex karyotypic abnormalities.
骨桥蛋白和骨钙素的表达也呈阳性,与活体组织原位杂交的实验结果相符合,表明牙囊细胞具有合成矿化组织的能力。
The results indicated that dental follicle cells not only exhibit fibroblastic characteristics, but also have some characteristics of osteoblasts or cementoblasts.
测定荧光原位杂交(FISH)分析法对现场未确定细胞病理学结论的患者的敏感性和特异性。
To determine the sensitivity and specificity of FISH analysis in patients with inconclusive on-site cytopathology results.
在免疫组化、原位杂交、细胞学检测等领域均有优异的表现。
The product has excellent performance in the fields of immunohistochemistry , in-situ hybridization, cytological detection, etc.
方法:取试管婴儿助孕技术后未能受精成功的卵细胞,采用多色荧光原位杂交方法检测卵细胞13,16 ,18,2 1和2 2号染色体的情况。
Methods:Multicolor fluorescence in situ hybridization (M-FISH) was applied to check the chromosome status in oocytes for chromome 13,16,18,21 and 22.
方法:取试管婴儿助孕技术后未能受精成功的卵细胞,采用多色荧光原位杂交方法检测卵细胞13,16 ,18,2 1和2 2号染色体的情况。
Methods:Multicolor fluorescence in situ hybridization (M-FISH) was applied to check the chromosome status in oocytes for chromome 13,16,18,21 and 22.
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