方法：牛晶体上皮细胞原代培养。
Methods: Bovine lens epithelial cells (BLEC) were cultured in vitro.
目的：探索人脂肪源性基质细胞原代培养方法。
Objective: To establish a primary human adipose-derived stromal cells culture method.
目的对大肠腺瘤细胞原代培养的方法作进一步的改进。
Objective To improve the primary cell culture methods of colorectal adenomas.
目的：建立牛视网膜毛细血管周细胞原代培养的方法。
Objective To establish a method for the primary culture of bovine retinal capillary pericytes.
结论添加晶体皮质可提高晶体上皮细胞原代培养成功率。
ConclusionAdding lens cortex can increase the success rate of primary culture of lens epithelial cells.
方法：分别采用组织块法、酶消化法、组织块酶消化法进行人牙髓细胞原代培养。
METHOD: Human pulp cells were primary cultured with tissue block method, enzymolytic method, and tissue block enzymolytic method separately.
分别采用酶消化法和组织块培养法，建立了猪小肠黏膜上皮细胞原代培养的方法。
To establish the method on the primary culture of porcine intestinal epithelial cell, different digestion protocols and explant culture were used to identify the best method.
分别采用酶消化法和组织块培养法，建立了猪小肠黏膜上皮细胞原代培养的方法。
The method of explant culture was used successfully in this study to establish neonate goat intestinal epithelial cell(IEC)line.
研究人员通过建立动物肝脏再生模型和肝细胞原代培养模型，逐渐发现多种效应因子。
By study on animal models of liver regeneration and primary hepatocytes culture, investigators have proved many types of effective factors.
前言：目的：比较不同的人牙髓细胞原代培养方法，优化培养条件，提高培养成功率。
AIM: to compare the primary culture methods of human pulp cells, to enhance the culture condition, and give rise of the success rate of cultivation.
方法采用人胚脑神经细胞原代培养，接种TCID50的HCMV后用光镜、组织染色观察病变全过程。
Methods The human embryo cerebral neurons were prepared for the primary culture using light microscope, tissue staining after inocula-ting HCMV of TCID50.
目的：探索人脑胶质瘤细胞原代培养的方法，提高原代培养的成功率，快速构建人脑胶质瘤细胞体外试验模型。
OBJECTIVE: to explore the method of primary human glioma cells culture, to improve the success rate of primary culture, and to establish an experimental model for studying glioma in vitro.
方法利用手术显微镜及显微手术器械分离大鼠肠系膜淋巴管。采用植块培养法进行内皮细胞原代培养并传代培养。
Methods With an operating microscope and microsurgical instruments, rat's mesenteric lymphatics were isolated and the endothelium were primarily cultured and subcultured.
方法：胚胎大鼠脊髓神经细胞原代培养，倒置相差显微镜下进行细胞记数和显微测量，观察神经元存活和生长分化的状况。
Methods: Using primary nerve cell culture, we observed the survival and growth of spinal cord neurons with phase-contract microscope.
方法采用神经元细胞原代培养，通过MTT比色法及LDH活力测定观察加入不同浓度人参总皂甙对神经元细胞的保护作用。
Methods To investigate the protective effects of Ginsenosides in different dose level on primary cultured rat cortical neurons through MTT colorimetry, activity detection of LDH.
目的：建立一种简单高效的中脑多巴胺神经元细胞原代培养方法，并观察胰酶消化对中脑多巴胺能神经元突起生长的损伤作用。
Objective: To establish an easy and high-yield method of rat dopaminergic neuron primary culture, and to observe the damage to the neurites by the trypsogen.
结论梯度离心法能成功分离胸腺树突状前体细胞，GMCSF和SCF体外诱导可提供胸腺树突状细胞原代培养较为满意的生长条件。
Conclusion the method of mechanically, gradient centrifugation can successfully separate the TDC. GM-CSF and SCF can promote the growth of the TDC and provide satisfactory growth conditions for them.
方法：膜片钳全细胞记录测量原代培养大鼠海马神经元膜的钾离子电流。
Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.
迄今为止我们已取得了突变的原代培养的细胞以及蠕虫”。
To date we have made mutant tissue-cultured cells as well as worms.
方法以人脐静脉内皮细胞（HUVEC）原代培养模型，MTT法测定紫杉醇对HUVEC增殖的影响；
Methods Human umbilical vein endothelial cells were cultured primarily in vitro and influence of Paclitaxel on HUVEC proliferation was evaluated by MTT method.
目的：探索原代培养大鼠肝细胞的最佳分离方法。
Aim: To explore the best isolation methods of rat liver cells from primary culture.
结果成功原代培养了猪眼rpe细胞。
Results Primary porcine RPE cells were cultured successfully.
结果：原代培养的RPE细胞含大量色素呈多角形，传代的细胞透明呈梭形。
Results: Primary cultured RPE cells contained large amount of pigments in polygonal shape. Passages of cultured RPE cells presented transparent spindle shape.
目的探索人心房肌细胞的原代及传代培养方法。
Objective To explore a method for primary culture and subculture of human atrial myocardial cells.
目的研究原代培养的脊髓神经干细胞在小鼠视网膜的整合和分化情况。
Objective to study the integration and differentiation of spinal neural stem cells after transplantation into mice retina.
目的：建立快速、有效的绒毛滋养细胞体外原代培养的方法以及建立人滋养细胞株和初步评价。
Objective:To establish the quick and valid primary culture method of human placental villus in vitro and set up a human trophoblastic cell line with preliminary assessment.
结论1、用原代培养的方法培养肾上腺细胞可获成功。
Conclusion: 1, the adrenal cells can be cultured by the methods of primary successfully.
本研究拟建立人嗜铬细胞瘤细胞的原代培养方法。
The study is to establish a method of separating and primarily culturing of human pheochromocytoma cells.
目的探讨体外原代培养大鼠肝细胞的分离方法。
ObjectiveTo explore the isolating methods of rat hepatocytes for primary culture.
说明原代培养细胞在血清中能分化为神经元及神经胶质细胞。
It demonstrated that some of the differentiated cells are neurons and the others were glial cells.
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