结论:超声驻波血细胞分离法与离心分离法相似,安全、可靠。
Conclusion Ultrasonic standing wave blood cell separation technology is safe and reliable, and is similar to centrifugal blood separation method.
方法采用改进的酶解分离法分离单个细胞并经膜片钳全细胞记录法进行鉴定。
Methods We used the improved enzymatic dissociation method and the patch clamp whole cell recording.
目的建立一种人肠系膜小动脉平滑肌细胞急性酶分离法并在分离的细胞上研究其离子通道特性。
Objective To establish an enzymatic isolation method of human mesenteric arteriolar smooth muscle cells and study ion channel characteristics of the isolated cells.
材料和方法:采用酶辅助的显微分离法分离新生牛眼ipe细胞,酶消化法分离角膜内皮细胞,进行体外细胞培养。
Material and Methods: From bovine eyes IPE cells were isolated with enzyme-assisted microdissection, corneal endothelium cells with enzyme digestion and then were cultured in vitro.
方法采用酶消化法及酶辅助机械分离法分别分离IPE和RPE细胞。
Methods IPE and RPE cells were isolated by enzymatic digestion method and enzyme-assisted microdissection method respectively.
目的建立脱脂棉柱纯化人外周血T淋巴细胞的方法,并与尼龙棉柱分离法进行比较。
Objective To establish a method to purify T lymphocytes from human peripheral blood mononuclear cells (PBMCs) using cotton column and compare its result with a nylon wool cotton column.
目前国外采用较多的有荧光激活细胞分选法、磁活化细胞分选法、免疫磁珠分离法、密度梯度离心法。
Various methods of enrichment exit:fluorescent activated cell sorting, magnetic-activated cell sorting, immunomagnetic beads, density gradient.
用消化分离法,利用计算机图像分析系统测心肌细胞的体积;
The cardiomyocytes' volumes was measured by computer photograph analysis system.
方法采用酶辅助机械分离法分离并培养兔眼虹膜色素上皮细胞。
Methods the IPE of rabbits were detached and cultivated according to the method of enzyme-assisted microdissection.
方法采用酶辅助机械分离法分离并培养兔眼虹膜色素上皮细胞。
Methods the IPE of rabbits were detached and cultivated according to the method of enzyme-assisted microdissection.
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