目的：介绍一种简单有效的单细胞分离技术。
OBJECTIVE: To introduce a simple and effective method for single cell separation.
掌握简单有效的单细胞分离技术为准确、定量的单细胞水平研究奠定基础。
A simple and effective method for single cell separation would be a foundation of exactly and quantitative study at single cell level.
传统的亚细胞分离技术结合质谱鉴定技术为亚细胞蛋白质组学的研究提供了技术平台。
The classical subcellular fractionation methods coupled with mass spectrometry identification technique have created opportunities for the study of subcellular fractions.
随着单细胞分离技术及单细胞测序技术日益成熟，单个肿瘤细胞全基因组测序已成为肿瘤研究的一个崭新的领域。
With the development of the technology of single-cell separation and sequence, sequencing single tumor cell has become a new area of cancer research.
为了解答这些问题，研究人员运用活细胞成像技术到人胚胎干细胞和鼠胚胎干细胞最初的分离阶段。
To answer these questions, the researchers applied live-cell imaging to the earliest phase of dissociation in human and mouse ES cells.
结论在本实验室，离心淘洗技术已能成功应用于肝脏细胞分离过程。
Conclusion Centrifugal elutriation technique could be applied successfully during the separation of liver cells in our laboratory.
然后利用独特的、临床可行的技术将患者血样中每种淋巴细胞类型分离成适合研究的纯样。
The team then used unique, clinically applicable techniques to separate out each immune cell type in the patients' blood into pure samples for study.
目的采用离心淘洗技术分离纯化大鼠肝非实质细胞亚群。
Objective In order to isolate and purify nonparenchymal cells subgroups from rat liver, centrifugal elutriation technique was applied.
方法：应用人表皮细胞的分离、培养技术，并进行MTT检测、病理切片以及电镜检测等手段进行观察。
Method: Use tissue engineering technology to separate and culture human keratinocyte in vitro and observe them using MTT method and histological and electron microscopic methods.
肝细胞通过毛地黄皂苷-胶原酶灌流技术分离。
Hepatocytes were isolated by the digitonin-collagenase perfusion technique.
在细胞里和在身体里理解蛋白质的功能使强有力的分离和分析的技术用先进的讯息科学结合。
It combines powerful separation and analytical technology with advanced informatics to understand the function of proteins in the cell and in the body.
方法实验采用的方法是全细胞膜片钳技术，实验材料为急性分离的牛蛙背根神经节神经元。
Method The experiment was carried out in freshly isolated bullfrog using a whole-cell patch-clamp technique.
表皮细胞分离培养技术的第一步就是要从皮肤中分离基底层细胞。
Isolation and culture of Keratinocyte, it is the first step to isolate the epidermal basal layer cells.
应用酶解技术将纤维质和果胶质水解，提取出细胞质和细胞间液，再经分离纯化可提取海带多糖。
The cytoplasm and intercellular fluid can be extracted from enzyme hydrolyzed cellulosic material and pectin, then the laminarin can be obtained via separation and purification.
方法从胚胎大鼠的大脑皮质、海马、纹状体等组织分离神经干细胞，用无血清培养技术在体外进行培养、扩增、传代和诱导分化。
The advantage of serum free and clone culturing technology was performed to isolate, culture, passage and induce neural stem cells from embryonic rat cortex, hippocampus and striatum.
方法：用膜片钳实验技术记录胶原酶急性分离的豚鼠心室肌细胞钾通道电流。
Methods The whole cell patch clamp technique was used in isolated ventricular cells of guinea pigs.
结论DADS能诱导人白血病HL 6 0细胞分化，并引起相关的基因发生改变，而抑制性消减杂交技术能有效地分离差异表达的基因。
CONCLUSION DADS can induce human leukemia HL 60 cells differentiation, and suppression subtractive hybridization (SSH) technique is an effective method to isolate those differentially expressed genes.
方法：利用无血清培养和细胞克隆培养技术，从胚胎大鼠海马、纹状体、脊髓等区分离神经干细胞，进行体外扩增培养、传代、贴壁分化观察。
Methods: The advantage of serum free and clone culturing technology was performed to isolate, culture and passage neural stem cells from embryonic rat hippocampus, striatum and spinal cord.
方法利用无血清悬浮培养、单细胞克隆技术分离神经上皮干细胞；
Methods Serum free cells suspension culture and single cell cloning technique were used to isolate neuroepithelial stem cells.
运用单细胞凝胶电泳技术，研究了太原市大气细颗粒物(PM 2.5)对分离的大鼠肺泡巨噬细胞DNA的损伤作用。
Damage from atmospheric fine particles (PM2.5) in isolated DNA of alveolar macrophages of rats in Taiyuan City was studied applying single cell gel electrophoresis technique.
方法：应用全细胞膜片箝电流箝制技术，对急性分离的大鼠DRG细胞进行电流箝制。
Methods: The isolated neurons of dorsal root ganglion (DRG) were studied using the whole cell patch clamp technique in current clamp mode.
目的探讨建立人皮肤汗腺细胞体外分离及纯化培养的技术。
Objective To investigate the method of isolation and purification of epithelial cells of human eccrine sweat gland in vitro.
前言：目的：建立分离猪淋巴管内皮细胞的技术及其培养方法。
Objective: To establish the technology of isolation of Lymphatic endothelial cells of pig thoracic duct and the culture method of their endothelium.
方法：采用标准酶分离技术和细胞培养技术分离培养兔内皮细胞。
METHODS: Rabbit endothelial cells were cultured by standard enzyme separation technique and cell culture technique.
笔者应用机械分离获得豚鼠单个初级精母细胞，采用全细胞式膜片钳技术记录该细胞离子通道电流。
Mechanical dissociation was used to obtain single primary spermatocytes from guinea pigs and iron channel currents in the primary spermatocytes were recorded by whole cell patch-clamp technique.
采用染色细胞分离的方法对该技术分离单细胞的效率进行验证。
Success rates of separation were evaluated by separated staining of single cells.
方法采用全细胞膜片钳技术在急性分离的大鼠背根神经节细胞上观察龙血素B对辣椒素诱发的辣椒素受体电流的影响。
Methods In acutely isolated rat DRG neurons, effects of loureirin B on capsaicin-evoked currents were observed using whole-cell patch-clamp technique.
借助超声分离技术，对细化前后牦牛纤维皮质细胞形态的变化进行分析，发现纤维皮质细胞被拉长变细且不发生细胞间的滑移。
The shape changes of cortical cell of yak hair fiber have been analyzed before and after stretching slenderization by means of ultrasonic separation.
方法：采用高阻抗密封膜片箝全细胞技术，记录分离的豚鼠和家兔心肌单个细胞的钾电流。
METHODS: A tight seal whole-cell patch-clamp technique was used to record ITO, IK, and IK1 in single cells isolated from guinea pig and rabbit hearts.
由于在体环境异常复杂，细胞生理或病理现象的研究仍依赖于细胞体外分离和培养技术，因此，构建细胞体外加载实验系统是目前研究细胞的力学响应机制的主要途径。
Because of the staggering complexity of the in vivo environment, studying cellular responses to mechanical stress and strain has relied heavily on the use of in vitro mechanical loading systems.
应用推荐