目的研究骺板软骨细胞冻存的方法。
Objective To study frozen reservation method of epiphyseal plate chondrocyte.
细胞冻存复苏后保持87%的存活率。
部分细胞冻存，另一部分细胞进行体外培养。
Some of the suspension cells were frozen and the others were cultured.
目的：观察细胞浓度对造血干细胞冻存效果的影响。
Objective:To obsreve the effect of cell concentration on hemopoietic stem cell cryopreservation.
目的研究视网膜干细胞冻存复苏后的成活率及再培养后的增生分化特性。
Objective To investigate the viability and the characters of proliferation and differentiation of retinal stem cells (RSCs) after cryopreservation and anabiosis.
而血清处理和细胞冻存过程中的技术细节若处理不当，会对细胞培养的效果产生较明显的影响。
But, serum handles and produces if cell frozen technology detail in storing process being not handled properly, being going to be to the cell cultivation effect more obvious effect.
鹿茸细胞采用常规细胞DMSO冻存法复苏贴壁率优于程序式冻胚仪冷冻法、乙二醇常规细胞冻存法。
The antler cell adopt cell routine DMSO frozen methods is superior to program frozen methods and ethylene glycol cell routine freeze methods.
经生长激素(GH)测定、细胞染色及计算机图像分析，证明胎儿垂体细胞冻存前、后经体外培养均有活性。
The results of growth hormone (GH) determination, cell stain and computer image analysis showed that cells of both fresh and cryopreserved pituitary were of biological viability.
方法：1。标本获取及分组采集107例白血病、20例MDS病人及20例正常对照组骨髓进行染色体培养、制备及细胞冻存。
Methods 1. Patient samples: Bone marrow or blood cells were obtained from 107 patients with leukemia, 20 patients with MDS and 20 controls for chromosome and frozen cell purpose.
但是，如果在英国，当每个人出生时，我们将其保留下来并冻存干细胞，我们将有在需要时，用于制造肾脏的冻存细胞。
But if we kept it and froze down the stem cells of everybody born in the UK, there would be cells that could build kidneys waiting for them, frozen, in case they ever needed them.
ACT小组也将实验中的这些胚胎组织冻存，使用分离的单细胞作为人类胚胎干细胞的来源。
The ACT team also froze the embryos and used the single cell that was removed as a source of human embryonic stem cells.
从癌症患者血液中提取数以百万计的干细胞并冻存。
Millions of stem cells are filtered from the blood of a cancer patient and frozen.
在细胞培养实验中，血清的处理和细胞的冻存这两方面的问题，是较为复杂也是比较容易出错的。
That the serum treatment and frozen cells exist in the cell cultivation, is comparatively complicated and comparatively easy to make mistakes.
因而证明，液氮冻存法是培养细胞长期保种的一个行之有效的方法。
Liquid nitrogen was shown to be an effective measure for long-term preservation of cultured hepatoma cells.
结论兔角膜缘上皮细胞可以在体外培养、冻存和复苏。
Conclusion the limbal corneal epithelial cells of rabbit could be cultivated, cryopreserved and resuscitated in vitro.
目的探讨三维组织细胞旋转培养系统(RCCS)对胚胎胰岛冻存复苏后质量的影响。
Objective To study the impact of rotary cell culture system (RCCS) on the quality and activity of fetal islets after cryopreservation and resuscitation.
本文报告了体外培养的人体肝癌细胞系(QGY- 7703)冻存于液氮。
A cultured cell line of human hepatoma (QGY-7703) preserved in liquid nitrogen for seven years has been successfully revived.
目的探讨不同冻存方法对脐血造血细胞的影响。
Objective To show the effects of different methods on preservation of cord blood hematopoietic cells below zero degree.
复苏后的内皮细胞的生长曲线与未冻存细胞的生长曲线无差异。
No difference was found in growth curves of postthawed endothelial cells and non-frozen ones.
以体外培养的布尔山羊和黄牛耳组织成纤维细胞为研究对象，进行了哺乳动物体细胞的冻存与冷藏试验。
The experiment of cryopreservation and cold storage of fibroblast cells cultured in vitro of Boer goat and yellow cattle′s ear skin was conducted.
它们能成为稳定的细胞系和长期冻存。
They can be propagated into stable cell lines and cryopreserved.
结论：在冻存液中加入10%DMSO和4%BSA，可在低温保存的过程中较好地保护细胞的存活及微囊的完整性。
Conclusion:The frozen stock solution with 10%DMSO and 4%BSA can preserve the cells and permeability of microcapsules in the process of low temperature preservation.
目的建立兔角膜缘上皮细胞体外培养、冻存和复苏的方法。
Objective to establish a method of cultivating limbal corneal epithelial cells of rabbit in vitro and to study its cryopreservation and resuscitation.
人骨髓冻存后其造血祖细胞活力有一定程度下降，本研究对这种下降的可逆性作了初步观察。
The reversibility of decreased viability of hemopoietic cells in human bone marrow after cryopreservation was studied.
细胞传代培养和冻存6个月，7td1MC 5细胞对IL - 6的反应性和依赖性均无明显变化。
No obvious variation of reactivity and dependence of 7td1 MC5 cell on IL-6 was observed after 6 months Subculture and Cryostorage.
试验对新建立的细胞系冷冻保存了一定数量的细胞，经冻存前和复苏后细胞活率检测，结果均在90%以上。
A certain extent number of cells for these two breeds were freezed, and viability examination of these cells showed that viable rates were higher than 90% before freezing and after recovery.
冻存不同时间的软骨细胞组之间无明显差异。
There was no obvious difference among the chondrocytes frozen at different time.
目的观察超低温冻存对人骨髓基质干细胞(BMSC)生物学特性的影响。
Objective To study the effects of cryopreservation on biological characters of human bone marrow stromal cell (BMSC).
结论深低温冻存的同种异体肌腱移植后异体细胞在关节外环境存活不超过8周。
Conclusion Viable donor cells in cryopreserved tendon allografts did not survive longer than 8 weeks following transplantation in an extra-articular environment.
通过冻存后复苏的细胞继续培养后发现，冻存对其生长、形态、遗传学等特征无明显影响。
After the cells were subcultured, the frozen storage didn't influence their growth, morphology, and genetic characteristics.
应用推荐