目的建立流式细胞术检测细胞内细胞因子的方法。
Objective To develop the technique for measuring intracellular cytokines by3 - colour flow-cytometry.
细胞内细胞因子检测的影响因素较多，分析时需注意选择适当的测定条件。
There are many influence factors in the method. It is noted to select the appropriate conditions at the analysis.
目的探讨CD4+T细胞内细胞因子在视神经脊髓炎及其临床分期中的特点及变化。
Objective To analyze intracellular cytokines of CD4 + T cells in neuromyelitis optica (NMO) during relapse phase and remission phase.
分别应用抗原刺激试验、酶联免疫反应、细胞内细胞因子染色检测上述T细胞的细胞因子表达。
The cytokine profiles of these T cells were detected by antigen stimulated assay, ELISA and intracellular cytokine staining.
目的：分析过继转输的小鼠胚胎抗原耐受T细胞内细胞因子及细胞表面协同刺激分子的表达特征。
Objective: to explore the expression characteristics of intracellular cytokines and surface costimulatory molecules in embryo antigen tolerant t cells transferred in pregnant mice.
通过二聚体染色和HBV肽刺激后的细胞内细胞因子染色测定肝和脾中HBV特异性CD 8 +T细胞的频率和功能。
The frequency and functionality of HBVspecific CD8 + t cells in the liver and spleen were determined by dimer staining and intracellular cytokine staining after stimulation with HBV peptides.
许多转录因子、细胞内信号转导调节蛋白、炎症细胞因子和细胞凋亡相关基因的表达均发生了明显的调节变化。
The expressions of many genes encoding transcription factor, cytokines, cell signaling modulators and apoptosis associated proteins were found to have changed.
许多转录因子、细胞内信号转导调节蛋白、炎症细胞因子和细胞凋亡相关基因的表达均发生了明显的调节变化。
The expressions of many genes encoding transcription factor, cytokines, cell signaling modulators and apoptosis associated proteins were found to have changed.
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