应用MTT法测定脾淋巴细胞转化指数。用免疫荧光法测定脾t淋巴细胞亚群细胞数，观察其对免疫功能的影响。
Spleen lymphocyte transforming index was measured with MTT method, and subtypes of t lymphocyte were counted by immunofluorescence to observe the influence on the immunological function.
方法采用APAAP桥联酶标法测定外周血t细胞亚群。
Methods t lymphocyte subpopulation in peripheral blood was measured by APAAP method.
目的测定围术期外周血T淋巴细胞亚群的变化，旨在探讨术中及术后连续硬膜外镇痛对免疫功能的影响。
Objective To investigate the influences of continuous epidural analgesia on immune function, authors observed the changes of peripheral blood T lymphocyte subsets during perioperative period.
方法采用多色流式细胞术结合血液分析仪测定38例SARS患者外周血淋巴细胞及其亚群的表达情况。
Method Flow cytometer and hematology analyzer were used to detect the expression of lymphocytes and its subsets in 38 patients of SARS.
方法：本研究采用间接免疫荧光法测定T细胞亚群；
Method:The indirect immunofluorescent assay was used in the present study.
方法应用单克隆抗体技术对32例乳腺癌患者进行了外周血T淋巴细胞亚群的测定，并与35名正常健康作对照。
Methods T lymphocyte subgroup level was determined by monoclon antibody in 32 cases of breast cancer and 35 normal controls.
方法分别采用流式细胞技术和散色比浊法测定HSP急性期与恢复期及对照组儿童血清免疫球蛋白浓度和T淋巴细胞亚群水平。
Methods The immunoglobulin concentration and T-cell subgroup level of acute stage and convalescent stage HSP children were detected with FCM and nephelometer.
方法采用SAP法分别测定28例老年acs患者、34例老年对照组(NC)的外周血t淋巴细胞亚群水平。
Methods The level of T-lymphocyte subpopulations in blood was assayed by SAP method from 28 senior citizens with ACS, 34 cases normal controls (NC).
方法分别采用双抗体夹心elisa法与抗体致敏的红细胞花环试验法，对55例慢性病毒性肝炎患者血清il - 12水平及T细胞亚群进行了测定。
Methods Serum IL-12levels and t subsets in55patients with chronic viral hepatitis were detected with double-sandwich ELISA and erythrocyte garland test respectively.
方法分别采用双抗体夹心elisa法与抗体致敏的红细胞花环试验法，对55例慢性病毒性肝炎患者血清il - 12水平及T细胞亚群进行了测定。
Methods Serum IL-12levels and t subsets in55patients with chronic viral hepatitis were detected with double-sandwich ELISA and erythrocyte garland test respectively.
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