方法组织块培养法。
组织块培养法对于原代细胞的培养要优于酶消化法。
The method of explanted tissue culture is better than the method of enzyme digestion for the primary cells.
结果建立了猪皮肤成纤维细胞系组织块培养法优于胰酶法。
Results Porcine skin fibroblast line was established and the tissue piece method was superior to trypsin methods.
经酶消化法短期培养成功率为83.3%，明显高于组织块培养法。
The successful rate of the enzymatic digestion culture method was 83.3%, which was prior to the tissue pieces culture method significantly.
方法采用显微解剖法分离出耳蜗血管纹，组织块培养法进行体外培养。
Methods Cochlea stria was separated by micrergy from guinea pigs and the stria tissue nubbles were cultured in vitro.
方法结合酶消化法和组织块培养法，体外单层培养人退变纤维环原代细胞。
Method:Primary cells of human degenerative AF were obtained through in vitro monolayer culture combined with enzyme digestion method and tissue pieces culture method.
方法：取瘢痕疙瘩和正常皮肤，采用组织块培养法进行成纤维细胞体外培养。
METHODS: Keloid and norm al skin tissue were collected for the fibroblasts in vitro using tissue-block cultivation.
通过组织块培养法，获得了传代培养的鲁西黄牛耳皮肤成纤维细胞和上皮细胞。
Luxi cattle-ear skin fibroblast and epithelial cells were derived by fragment of tissue.
方法：取5-10周流产人胚胎生殖腺嵴，采用组织块培养法培养人EG细胞。
Methods: Through tissue culture, the human EG cells were obtained by taking the gonadal ridges from human embryos aged 5~10 weeks.
分别采用酶消化法和组织块培养法，建立了猪小肠黏膜上皮细胞原代培养的方法。
To establish the method on the primary culture of porcine intestinal epithelial cell, different digestion protocols and explant culture were used to identify the best method.
分别采用酶消化法和组织块培养法，建立了猪小肠黏膜上皮细胞原代培养的方法。
The method of explant culture was used successfully in this study to establish neonate goat intestinal epithelial cell(IEC)line.
方法采用组织块培养法培养羊膜细胞，酶消化法对细胞进行传代，应用免疫细胞化学的方法对细胞进行鉴定。
Methods Amnion cells from human were cultured with tissue piece method, passaged by trypsin digestion and identified with immunocytochemistry.
结果：倒置显微镜观察，悬浮细胞培养法A组和组织块培养法B组扩增的角膜缘上皮细胞均能在羊膜上单层生长。
Results: A monolayer of cultured corneal limbal epithelial cells was observed by inverted microscope in both A group and B group.
结果两种方法均能得到乳腺癌原代细胞，组织块培养法的成功率为90.0%，细胞培养法的成功率为20.0%。
Results the breast carcinoma cells could be cultured by these two methods with a success rate of 90% in the tissue culture method and that of 20% in the cell culture method.
方法用组织块法培养人瘢痕成纤维细胞。
Methods The hypertrophic scar fibroblasts were cultured with tissue culture method.
资料综合：许旺细胞的培养方法包括组织块法和酶消化培养法。
DATA SYNTHESIS: The Schwann cells could be cultured by tissue block method and enzymes digesting method.
方法血管平滑肌细胞采用组织贴块法培养。
Methods Vascular smooth muscle cells were cultured by explant method.
用组织块直接培养法，也可以得到皮肤的原代培养细胞。
The primary culture cells of rabbit skin tissue could be obtained by explant culture too.
方法：分别采用组织块法、酶消化法、组织块酶消化法进行人牙髓细胞原代培养。
METHOD: Human pulp cells were primary cultured with tissue block method, enzymolytic method, and tissue block enzymolytic method separately.
采用组织块贴壁培养法，成功地构建北京鸡成纤维靶细胞系。
Using the organization to adherent culture, we succeeded in constructing Beijing fatty chicken fibroblast specific target cell.
结论：在对根、冠髓细胞的原代培养中，组织块法优于组织块酶解法。
CONCLUSIONS: tissue explant method was superior to tissue explant-collagenase digestion method for the culturing of human dental coronal and root pulp cells.
方法采用低钙培养法对人角膜缘基底层细胞组织块进行培养。
Methods Limbal button with basal cells was cultured in low calcium medium.
采用组织贴块法分离培养兔平滑肌细胞。
Rabbit smooth muscle cells were isolated and cultured using tissue explant method.
方法：采用组织块贴壁法培养大鼠主动脉平滑肌细胞。
Methods: VSMCs were cultured by tissue explants adherent method.
用胰蛋白酶和胶原酶消化法和组织贴块法培养猪肺动脉干内皮细胞和平滑肌细胞。方法简便易行，成功率高。
Endothelial cells and smooth muscle cells of porcine pulmonary trunk were cultured by enzyme-dispersed and substrate-attached explant methods.
改良组织块法是一种良好的大鼠牙囊细胞培养方法。
Improved explant culture was a good culturing method for rat DF cells.
改良组织块法是一种良好的大鼠牙囊细胞培养方法。
Improved explant culture was a good culturing method for rat DF cells.
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