提纯获得了纯化病毒，并免疫家兔制备出抗血清。
The virus was purified and antiserum was prepared by immunized rabbit.
这病毒外部蛋白然后被提纯并产生几百到几千毫升的纯化病毒蛋白，作为抗原，是疫苗中的活性成分。
The outer proteins of the virus are then purified and the result is several hundred or thousand liters of purified virus protein that is referred to as antigen, the active ingredient in the vaccine.
方法将森林脑炎病毒感染地鼠肾单层细胞，收获病毒液，经超滤浓缩、柱层析纯化病毒后配制而成。
Methods Hamster kidney cells were infected with tick - borne encephalitis virus, and the harvested virus suspension was concentrated by ultrafiltration and purified by column chromatography.
用化学品杀死不完全纯化的病毒。
在病毒和毒物攻击器官时血液纯化仪就滤出病毒。
It is designed to filter out viruses and toxins before they attack organs.
近年来又发现了果胶酸裂解酶还可用于植物病毒的纯化、纸浆漂白和以纺织品的生物精炼等。
It was also found recently that the pectate lyase could be used for the purification of plant virus, bleaching of paper pulp and the bio-refining of textiles etc.
应用elisa和病毒毒力滴度测定两种方法对狂犬病疫苗纯化过程中各步样品进行检测，并应用NIH效力测定法对ELISA法测定的结果进行验证。
Different level examples of rabies virus during purification process were tested by ELISA and virus infectivity titre. and the results were compared with that of results of NIH.
然后是除菌级滤器验证的章节，随后的章节提到了用来分离（纯化）和除病毒的滤器验证。
Next is a section on the validation of sterilizing-grade filters. Subsequent sections address validation of filters used for clarification and virus removal filters.
用亲和层析纯化的猪繁殖与呼吸综合征病毒(PRRSV)重组核衣壳蛋白gst - N作为诊断抗原，建立检测prrs血清抗体的间接ELISA方法。
Using recombinant nucleocapsid protein GST-N of porcine reproductive and respiratory syndrome virus (PRRSV) as antigen, an indirect ELISA for the detection of antibodies against PRRSV was developed.
本研究以从山西西瓜上分离纯化得到的西瓜花叶病毒2号（WMV-2）分离物为材料，用RT-PCR方法得到其外壳蛋白（CP）。
A watermelon mosaic virus 2 (WMV-2) isolate from diseased watermelon in Shanxi Province was used for cDNA synthesis of WMV-2 CP gene by RT-PCR.
方法从Z 10株病毒感染的细胞提取总rna，将逆转录pcr扩增的产物纯化后克隆于t载体并进行序列测定，应用dnastar软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR product was cloned into t vector, sequenced and analyzed using DNASTAR software.
方法从Z 10病毒感染的细胞提取总rna，将逆转录聚合酶链反应(RT PCR)扩增的产物纯化后克隆于t载体并进行序列测定，应用dnas TAR软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR products was cloned into t vector, sequenced and analyzed by using DNASTAR software.
方法利用传统鸡胚培养方法制备新城疫病毒（NDV），通过PEG-6000沉淀，差速离心和蔗糖密度梯度离心等方法提取纯化了新城疫病毒（NDV）。
Methods Newcastle disease virus(NDV) was prepared in embryonic egg. NDV was purified by deposited of PEG-6000, speed centrifugation and sucrose—density gradient—centrifugation(SDGC).
目的利用几种助沉剂和核酸纯化方法富集病毒rna，建立甲型H1N 1流感超敏感诊断方法。
Objective To establish a super-sensitive diagnosis method for influenza a (H1N1) by enrichment of virus RNA using several settling agents and nucleic acid purification methods.
经一次差速离心分别提取鸡胚尿囊液中的F_（48）E_8和N-79株鸡新城疫病毒（NDV），获得纯化抗原。
The Newcastle Disease viruses (NDV) of strains F48Eg and N-79 prepared from embryonated eggs were purified by one differential centrifugation.
原核表达并纯化单纯疱疹病毒特异性糖蛋白抗原，用于建立单纯疱疹病毒的临床检测方法。
To express and purify the specific glycoprotein antigens of herpes simplex virus (HSV) as used in HSV clinical detection.
结论：改进的方法是一种高效、简便、快捷地包装并纯化重组溶瘤腺病毒的方法，采用该方法包装的重组溶瘤腺病毒能满足实验的需要。
Conclusion: It is a simple, rapid and high efficient method for the packaging and cloning purification of recombination adenovirus, which meet the demands of subsequent experiments.
将腮腺炎病毒S79株培养液用中空纤维超滤器进行浓缩，经PEG沉淀后，采用蔗糖密度梯度离心法纯化抗原；
The tissue culture of mumps virus S79 strain was purified by ultrafiltration on hollow fiber, PEG precipitation and density gradient ultracentrifugation.
方法设计出针对各片段的特异性引物，用P CR方法从Z 37病毒感染的细胞提取细胞总rna，逆转录扩增、产物克隆t载体，纯化后测序，测定的序列应用DNASTAR软件比较分析。
Methods the total RNA was extracted from Z37 virus infected cells and the RT-PCR products were cloned into t vector, sequenced and analyzed by DNASTAR software.
目的获得纯化的新城疫病毒，为下一步病毒的应用打下基础。
Objective to acquire purified NDV virus to be foundation for next research.
本研究使用纯化的鸡传染性支气管炎病毒（IBV），从12肽噬菌体展示随机肽库中筛选特异性结合多肽。
This study utilized purified IBV H52 strain to screen specific virus-binding peptides from Ph. D. -12TM phage display peptide library.
证实了大孔吸附树脂吸附法纯化的微生物发酵液中小分子化合物粗提物具有不同程度的流感病毒神经氨酸酶抑制活性。
The small molecules can be greatly enriched and the amount of fermentation liquid required in resin purification method is much less than that of organic reagent extraction.
纯化的病毒亚单位用核酸酶消化后，与等量的福氏佐剂混合，免疫家兔制备抗体。
Afterwards, the preparation digested by RNase was equally mixed with Freund's adjuvant and immunized rabbits.
方法将特异性引物从S8546病毒感染细胞PCR扩增的产物克隆于T载体，正确的克隆纯化后测序，应用DNASTAR软件比较分析。
Methods RT PCR products were amplified from S85 46 virus infected cells, cloned into T vector, sequenced and analyzed using DNASTAR software.
该重配病毒免疫雌性山羊后获得较好的抗血清，且对纯化该抗血清的方法进行了探索。
The reassortant also has a higher yield via microcarrier culture. We could get the better anti-serum by immuning female goat, and selected suitable purification method of the anti-serum.
结论成功表达并纯化了埃博拉病毒科特迪瓦型核蛋白，为后续研究奠定了基础。
In conclusion, successfully expressed and purified Ebola virus nucleoprotein type Cted 'ivoire aid the foundation for the followup study.
目的通过对人类疱疹病毒（HHV）-6B纯化后鉴定手段的比较，筛选或优化出简单、快速、准确特异的方法。
Objective To select or optimize simple, fast and special means by compare of identification methods after purification of Human herpesvirus-6B.
目的通过对人类疱疹病毒（HHV）-6B纯化后鉴定手段的比较，筛选或优化出简单、快速、准确特异的方法。
Objective To select or optimize simple, fast and special means by compare of identification methods after purification of Human herpesvirus-6B.
应用推荐