建立了对发酵液中的普那霉素进行分离纯化的硅胶柱层析方法。
The separation and purification of pristinamycins from the fermentation broth by the method of silica gel column chromatography was developed.
最后经HA柱层析，得到高纯度的蒜氨酸酶，最终纯化倍数为20.68倍，回收率为38.53%。
Ultimately, alliinase was purified up to 20.68 times with a recovery of 38.53% by HA chromatography.
确立了以有机溶剂提取海藻中的生长素，以乙醚萃取法和柱层析法对生长素进行分离纯化的方法。
The method of extracting the auxin in seaweeds with organic solvent, separating and purifying the auxin by ether extraction method and column chromatography has been established.
将含量较高的前二者进一步纯化，乙酸纤维膜电泳和葡聚糖凝胶g- 75柱层析的结果表明纯化后的褐藻酸钠、褐藻糖胶是均一的。
Sodium alginate and fucoidin were further purified, the purity of which was demonstrated homogeneously by chromatography on a sephadex G-75 column and acetate cellulose membrane electrophoresis.
本实验用CF-11纤维素柱层析，从白萝卜提取物中分离纯化到一种双链RNA，简称E。
Using cellulose (CF-11 ) chromatography a double stranded RNA was purified from the extract of R. sativus-root, termed E.
方法：利用柱层析、结晶从山栀子的成熟果实中进行分离纯化栀子苷。
Methods: Silica gel column chromatography and crystallization were used to obtained gardenoside from Gardenia jasminoides.
提取液经柱层析分离及薄层层析和纸层析进一步纯化。
The extracts were separated by silica gel column chromatography, thin-layer chromatography and paper chromatography.
采用柱层析法分离纯化番茄红素。
Separation and purification of lycopene by column chromatogram were studied.
采用碱裂解法与DEAE -纤维柱层析法提取与纯化抑制素质粒dna，用分光光度法测定其浓度和纯度。
The inhibin plasmid DNA was extracted and purified by alkaline lysis method and DEAE-fiber column chromatography and its concn.
用硅胶柱层析法分离纯化，用光谱法进行结构鉴定。
METHODS ? The isolation and purification was performed by silica gel column chromatography and the structures were identified by spectral analysis.
方法应用柱层析及RP- HPLC，从眼镜蛇毒粗毒中分离纯化细胞素素（CTX）。
METHODS CTX-dwas fractionated and purified from Naja naja atra venom by column chromatography and RP-HPLC.
研究了通过大孔吸附树脂纯化苹果中原花青素柱层析的最佳条件。
We studied the optimum conditions for separating procyanidins from apple juice by macroporous resin.
用离子交换柱层析法分离，用反相高效液相色谱法进一步纯化，经聚丙烯酰胺凝胶电泳分析确定相对分子质量。
Methods The peptide was separated by ion exchange column chromatography and further purified by reverse phase HPLC, and the molecular weight was determined by tricine gel electro phoresis.
方法运用重结晶和柱层析法进行分离和纯化。
METHODS Recrystallization and column chromatography were used to get purer hyodeoxycholic acid and some other components.
总收率72％，比原分步工艺高16％，目标产品纯化不需柱层析。
The total yield was 72%, and 16% higher than the original reactions. The product can be purified without colum chromatograph.
方法采用甲醇索氏提取，中性氧化铝柱层析分离纯化、高效液相色谱法测定连翘中连翘苷含量。
METHODS The content of phillyrin in forsythia was determinated by using Soxhlet extractor with methanol, separation and purification with the neutral aluminum oxide column and HPLC method.
综合利用吸附法、溶媒萃取法和硅胶柱层析法对抗生素进行了纯化。
Finally, the antibiotics were purified by adsorption, solvent extraction and silica gel column chromatography.
酶经渗透压法破碎、硫酸铵沉淀和柱层析纯化。
The enzyme is crushed in osmotic pressure process, and purified in ammonium sulfate precipitation and chromatographic process.
采用硅胶、聚酰胺、葡聚糖凝胶LH-20柱层析法和制备薄层层析法对乙酸乙酯和正丁醇提取物进行了分离、纯化。
The ethyl acetate and n-butanol extraction were chromatographed on silica gel column, polyamide column, sephadex LH-20 column and preparing TLC, respectively.
方法：采用离子交换柱层析和凝胶柱层析纯化得到RRTP；
Method: RRTP-1 was obtained by ion exchange column chromatography and gel chromatography.
方法将森林脑炎病毒感染地鼠肾单层细胞，收获病毒液，经超滤浓缩、柱层析纯化病毒后配制而成。
Methods Hamster kidney cells were infected with tick - borne encephalitis virus, and the harvested virus suspension was concentrated by ultrafiltration and purified by column chromatography.
方法将森林脑炎病毒感染地鼠肾单层细胞，收获病毒液，经超滤浓缩、柱层析纯化病毒后配制而成。
Methods Hamster kidney cells were infected with tick - borne encephalitis virus, and the harvested virus suspension was concentrated by ultrafiltration and purified by column chromatography.
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