方法运用序列特异性引物聚合酶链反应技术，检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的HLA-DQB1等位基因。
METHODS HLA-DQB1 gene polymorphisms were typed by sequence specific primer based polymerase chain reaction, in 42 patients with esophageal neoplasm and 136 normal control subjects.
方法采用基因克隆、测序及生物信息学技术，分析HLA新等位基因与HLA已知基因序列的差异。
Methods Molecular cloning, sequencing and bioinformatics techniques were used to identify the difference between the new HLA allele and other known alleles.
方法利用聚合酶链反应-序列特异引物(PCR -ssp)法，对189例银屑病患者和273例健康人的HLA - DQA1和DQB1等位基因进行检测。
Method Polymerase chain reaction sequence specific primers (PCR-SSP) method was used to analyze the frequencies of HLA-DQA1 and DQB1 alleles among 189 patients with PV and 273 healthy controls.
通过测序确认基因座核心重复序列及等位基因核心序列重复数。
We have confirmed the core repeats and its repeating number of these alleles by sequencing.
方法运用聚合酶链反应-序列特异性引物（PCR-SSP）法，对38例山东汉族人GPP与94例健康对照进行HLA-DQB1等位基因分型。
Methods The distributing frequencies of HLA-DQB1 alleles were detected with polymerase chain reaction-sequence specific primers (PCR-SSP) in 38 GPP patients and 94 healthy subjects from Shandong.
方法应用PCR -STR和DNA序列分析技术，对4650个无关个体在15个STR基因座中的罕见等位基因进行检测。
Methods PCR-STR and DNA sequencing technology were used to detect rare alleles at 15 STR loci for 4650 unrelated individuals.
方法应用PCR -STR和DNA序列分析技术，对4650个无关个体在15个STR基因座中的罕见等位基因进行检测。
Methods PCR-STR and DNA sequencing technology were used to detect rare alleles at 15 STR loci for 4650 unrelated individuals.
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