方法运用序列特异性引物聚合酶链反应技术，检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的HLA-DQB1等位基因。
METHODS HLA-DQB1 gene polymorphisms were typed by sequence specific primer based polymerase chain reaction, in 42 patients with esophageal neoplasm and 136 normal control subjects.
方法利用聚合酶链反应-序列特异引物(PCR -ssp)法，对189例银屑病患者和273例健康人的HLA - DQA1和DQB1等位基因进行检测。
Method Polymerase chain reaction sequence specific primers (PCR-SSP) method was used to analyze the frequencies of HLA-DQA1 and DQB1 alleles among 189 patients with PV and 273 healthy controls.
目的建立一种简便、实用的检测亚甲基四氢叶酸还原酶(MTHFR)等位基因C677 T点突变的方法，并初步观察部分健康老人和老年血管性痴呆(VD)患者中mthfr等位基因C677 T点突变情况。
Objective to establish a simple and practical method for detecting the MTHFR gene C677T mutation so as to investigate MTHFR genotypes in the healthy elder and Vascular Dementia (VD).
方法运用聚合酶链反应-序列特异性引物（PCR-SSP）法，对38例山东汉族人GPP与94例健康对照进行HLA-DQB1等位基因分型。
Methods The distributing frequencies of HLA-DQB1 alleles were detected with polymerase chain reaction-sequence specific primers (PCR-SSP) in 38 GPP patients and 94 healthy subjects from Shandong.
方法在140名汉族健康人的外周血中，应用聚合酶链反应（PCR）限制性片段长度多态性分析（RFLP）及多重PCR技术，进行NAT1等位基因分型研究。
Methods Using multiple PCR and PCR-RFLP methods, we studied the NAT1 genotypes and its genetic polymorphisms of the peripheral blood samples from 140 Han people.
本研究利用聚合酶链式反应技术，成功地克隆了枯草芽孢杆菌缺陷型原噬菌体PBSX阻遏基因及其温度敏感型等位基因。
The repressor gene and its allele-a temperature sensitive mutant have been cloned from the defective prophage PBSX of Bacillus subtilis by means of PCR technique.
方法应用聚合酶链反应限制性片段长度多态性技术检测296例两个基因多态位点的等位基因、基因型。
MethodGenotypes and alleles of polymorphisms of both genes were determined with polymerase chain reactionrestriction fragment length polymorphism assay (PCRRFLP) of 296 subjects in Han Chinese.
方法采用等位基因特异性聚合酶链反应(AS - PCR)及基因测序方法检测190例MPD患者的MPLW515L和JAK2V 617 F点突变。
Methods MPLW515L and JAK2V617F mutations were simultaneously detected by alleles specific polymerase chain reaction (AS-PCR) in 190 MPD patients.
等位酶分析结果表明：1.在供试材料的6个种中，在11个酶系统的24个位点上共检测到69个等位基因，位点最大等位基因数为8。
Results of allozyme analysis showed: 1. 69 alleles were detected at 24 loci of 11 enzyme systems in 6 species, with the largest number of 8 in one locus.
用2种酶系统分别对中国李进行单株聚类分析，中国李某些性状与等位酶基因位点或等位基因的相关性有待进一步研究。
In order to identify enzyme systems related to some traits of Chinese plum, we used 2 enzyme systems for individual UPGMA cluster analysis. But their collection remains to be investigated.
两种同工酶（EST和POD）对31份乌拉尔甘草种质资源材料共检测到6个基因位点，19个等位基因，其中有15个多态性等位基因，多态性等位基因频率为78.95%。
Two isozymes (EST and POD) on 31 Guralensis were assessed, and 6 enzyme loci were identified, presenting 19 allozymes, including 15 polymorphic allozymes. Polymorphism rate of allzymeis 78.95%.
两种同工酶（EST和POD）对31份乌拉尔甘草种质资源材料共检测到6个基因位点，19个等位基因，其中有15个多态性等位基因，多态性等位基因频率为78.95%。
Two isozymes (EST and POD) on 31 Guralensis were assessed, and 6 enzyme loci were identified, presenting 19 allozymes, including 15 polymorphic allozymes. Polymorphism rate of allzymeis 78.95%.
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