方法运用序列特异性引物聚合酶链反应技术,检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的HLA-DQB1等位基因。
METHODS HLA-DQB1 gene polymorphisms were typed by sequence specific primer based polymerase chain reaction, in 42 patients with esophageal neoplasm and 136 normal control subjects.
方法运用聚合酶链反应-序列特异性引物(PCR-SSP)法,对38例山东汉族人GPP与94例健康对照进行HLA-DQB1等位基因分型。
Methods The distributing frequencies of HLA-DQB1 alleles were detected with polymerase chain reaction-sequence specific primers (PCR-SSP) in 38 GPP patients and 94 healthy subjects from Shandong.
方法利用聚合酶链反应-序列特异引物(PCR -ssp)法,对189例银屑病患者和273例健康人的HLA - DQA1和DQB1等位基因进行检测。
Method Polymerase chain reaction sequence specific primers (PCR-SSP) method was used to analyze the frequencies of HLA-DQA1 and DQB1 alleles among 189 patients with PV and 273 healthy controls.
方法采用等位基因特异性聚合酶链反应(AS - PCR)及基因测序方法检测190例MPD患者的MPLW515L和JAK2V 617 F点突变。
Methods MPLW515L and JAK2V617F mutations were simultaneously detected by alleles specific polymerase chain reaction (AS-PCR) in 190 MPD patients.
方法采用等位基因特异性聚合酶链反应(AS - PCR)及基因测序方法检测190例MPD患者的MPLW515L和JAK2V 617 F点突变。
Methods MPLW515L and JAK2V617F mutations were simultaneously detected by alleles specific polymerase chain reaction (AS-PCR) in 190 MPD patients.
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