目的：研究建立等位基因特异性引物pcr技术体系，并将其应用于基因单核苷酸多态性研究工作。
Objective: to study and establish an allelic specific primer polymerase chain reaction (ASP-PCR) technique system and to apply this technique to study single nucleotide polymorphism (SNP) of genes.
通过分析肿瘤细胞的密度及等位基因突变的几率来确定肿瘤异质性和等位基因特异性突变的不平衡性。
Tumor cellularity and mutant allele frequency were analyzed to identify tumor heterogeneity and mutant allele-specific imbalance.
目的：(1)建立使用等位基因特异性引物方法检测KLOTHO基因单核苷酸多态性的PCR反应体系。
Objective: (1) to establish PCR reaction system that USES allele-specific primer PCR technique to detect SNP of KLOTHO gene.
等位基因突变几率预测肿瘤异质性方面低于预期，但等位基因突变几率预测等位基因特异性突变不平衡性方面则高于预期。
Lower than expected mutant allele frequency indicated tumor heterogeneity, while higher than expected mutant allele frequency indicated mutant allele-specific imbalance.
新一代测序技术对等位基因特异性突变几率非常精确，检测出了23%的基因突变、其中等位基因突变的几率达2- 20%。
Next-generation sequencing showed precise measurement of mutant allele frequencies and detected 23% of mutations with 2-20% mutant allele frequencies.
方法采用等位基因特异性聚合酶链反应(AS - PCR)及基因测序方法检测190例MPD患者的MPLW515L和JAK2V 617 F点突变。
Methods MPLW515L and JAK2V617F mutations were simultaneously detected by alleles specific polymerase chain reaction (AS-PCR) in 190 MPD patients.
这些等位基因的分布具有地域特异性和种族特异性。
The distribution of these alleles is region specific and race specific.
方法运用聚合酶链反应-序列特异性引物（PCR-SSP）法，对38例山东汉族人GPP与94例健康对照进行HLA-DQB1等位基因分型。
Methods The distributing frequencies of HLA-DQB1 alleles were detected with polymerase chain reaction-sequence specific primers (PCR-SSP) in 38 GPP patients and 94 healthy subjects from Shandong.
方法运用序列特异性引物聚合酶链反应技术，检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的HLA-DQB1等位基因。
METHODS HLA-DQB1 gene polymorphisms were typed by sequence specific primer based polymerase chain reaction, in 42 patients with esophageal neoplasm and 136 normal control subjects.
等位基因频率说明了特异性位点与地理位置有关。
Aelle frequency proved particular loci have relation with geographical location.
等位基因频率说明了特异性位点与地理位置有关。
Aelle frequency proved particular loci have relation with geographical location.
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