逆转录病毒；稳定转染；干细胞。
Retrovirus vector; Stable transfected cell line; Stem cells.
大鼠骨髓间质干细胞可以成为PEX基因稳定转染的载体细胞吗？
Can bone marrow mesenchymal stem cells be used as PEX gene stable transfection vectors?
目的：在中国仓鼠卵巢(CHO)细胞上稳定转染咪唑啉1受体。
Objective: to stably transfect imidazoline-1 receptor (I 1r) in Chinese hamster ovary (CHO) cells.
方法细胞瞬时和稳定转染及氯霉素酰基转移酶和芳香族化酶活性测定。
Methods Transient and stable transfection experiments in cells and assays of chloramphenicol acetyltransferase activity and aromatase activity were used.
该稳定转染细胞系的建立为G250在肿瘤免疫治疗中的应用奠定了研究基础。
Stable transfection of mouse B16 cell line can highly express G250, thus laying a foundation for the application of G250 in immunotherapy for tumors.
目的：研究稳定转染外源性KAI1基因对人乳腺癌细胞体外生物学特性的影响。
Objective: To investigate the effect of stable transfection of the exogenous wild KAI1 gene on biological behavior of the breast cancer cell in vitro.
方法构建了不同TPR串联簇的截断突变体的表达质粒后，建立了稳定转染的BEL740 2细胞株。
Methods the plasmids which can express the truncated mutants of the TPR tandem clusters at different region of CRLP were constructed, stable transfected BEL7402 cell lines were established.
目的：构建人死亡受体5 (DR5)真核表达载体，转染NS - 1细胞，建立稳定转染的NS - 1细胞系。
Objective: to construct eukaryotic expression vector of human death receptor (DR5) and transfect NS-1 cells to establish stable NS-1 cell line.
目的构建人黑色素浓集激素1型受体（MCHR1）真核表达载体，转染CHO细胞，建立稳定转染的CHO细胞系。
Objective To construct eukaryotic expressing vector of human melanin-concentrating hormone receptor 1(MCHR1), then to transfect CHO cells with the vector for establishment of stable CHO cell line.
结果单、双自杀基因均在GLC- 82细胞中稳定表达，双基因转染组对细胞增殖的杀伤及旁杀伤效应高于单基因组。
Results Double and single suicide gene transfer were both stably expressed in GLC-82 cells. The cytotoxic effects of co-expressed TK-CD genes were superior than that of the single gene.
结论LPD是一种制备工艺简单、体外稳定性好、转染率高，具有应用潜力的非病毒载体系统。
Conclusion LPD could be prepared easily with small particle sizes and high transfection activities. LPD may be good non-viral vectors for applications in gene delivery.
在4株转染的COS7细胞株中有2株同样具有高效稳定的TIF3编码蛋白质表达。
Similarly, 2 out of 4 COS7 cell lines had significant over expression of TIF3 encoded protein.
结论成功构建稳定沉默CXCR4表达的肾癌a 498细胞株，转染后的细胞凋亡率升高，体外侵袭能力降低，为后续研究奠定了基础。
ConclusionWe have successfully established an A498 cell line with CXCR4 stably silenced; the cell line has a higher apoptotic rate and lower invasion ability, which paves a way for future research.
纳米控释系统使转染基因的胞内摄入量增高，增强其稳定性、靶向性及细胞定位。
Nano-delivery system increases cellular uptake of specific gene, protects them against nucleases and enhances their targeting and selectivity.
纳米控释系统使转染基因的胞内摄入量增高，增强其稳定性、靶向性及细胞定位。
Nano-delivery system increases cellular uptake of specific gene, protects them against nucleases and enhances their targeting and selectivity.
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