• 方法应用肠道细菌侵袭检测、宋内菌大质粒DNA图谱分析体口服耐受试验对S7S7R株进行安全性稳定分析。

    Methods Making use of enterobacterial virulence and invasive test, DNA profile analysis and challenge test of rhesus monkey to determine the safety and stability of S7 and S7R.

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  • 目的构建以融合蛋白形式大肠杆菌高效表达重组质粒稳定高效地获得基因工程产品素。

    Objective To construct recombinant plasmid with human atrial natriuretic peptide (ANP) gene in fusion form for stable and high level expression of genetic engineering product ANP in E. coli system.

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  • 重组菌中重组稳定进行研究结果表明该质粒宿主菌中具有良好的分离稳定性,结构稳定性较差。

    The plasmid stability studies showed that recombinant plasmid has excellent segregational stability but the structural stability was not good in the host cell.

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  • 方法构建了不同TPR串联截断突变体表达后,建立稳定转染的BEL740 2细胞株。

    Methods the plasmids which can express the truncated mutants of the TPR tandem clusters at different region of CRLP were constructed, stable transfected BEL7402 cell lines were established.

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  • 发酵过程中维持低葡萄糖水平可以限制细胞的生长速率,提高质粒稳定促进青霉素G化酶的合成。

    Methods:The influence of ammonium sulfate and glucose on plasmid stability and penicillin G acylase activity were conducted in flask shakes and a 5L fermentor.

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  • 结论成功地构建杜氏利什曼原虫鞭毛体蛋白基因真核表达重组质粒,并且该基因NIH3T3细胞中获得了稳定表达

    CONCLUSION: a recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed, and can be expressed stably in the NIH3T3 cells.

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  • 质粒经过稳定筛选稳定表达于细胞内,促进人肝细胞生长抑制凋亡肝脏来源的对照组细胞没有明显影响

    PEG10 gene accelerated the growth of L02 cells and inhibited their apoptosis but it had no conspicuous effect on the non-liver derived cells.

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  • 质粒经过稳定筛选稳定表达于细胞内,促进人肝细胞生长抑制凋亡肝脏来源的对照组细胞没有明显影响

    PEG10 gene accelerated the growth of L02 cells and inhibited their apoptosis but it had no conspicuous effect on the non-liver derived cells.

    youdao

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