方法应用肠道细菌毒力和侵袭力检测、宋内菌大质粒DNA图谱分析和猴体口服耐受试验对S7株、S7R株进行安全性和稳定性分析。
Methods Making use of enterobacterial virulence and invasive test, DNA profile analysis and challenge test of rhesus monkey to determine the safety and stability of S7 and S7R.
目的构建以融合蛋白形式在大肠杆菌中高效表达心钠素的重组质粒，稳定高效地获得基因工程产品心钠素。
Objective To construct recombinant plasmid with human atrial natriuretic peptide (ANP) gene in fusion form for stable and high level expression of genetic engineering product ANP in E. coli system.
对重组菌中重组质粒的稳定性进行研究，结果表明该质粒在宿主菌中具有良好的分离稳定性，而结构稳定性较差。
The plasmid stability studies showed that recombinant plasmid has excellent segregational stability but the structural stability was not good in the host cell.
方法构建了不同TPR串联簇的截断突变体的表达质粒后，建立了稳定转染的BEL740 2细胞株。
Methods the plasmids which can express the truncated mutants of the TPR tandem clusters at different region of CRLP were constructed, stable transfected BEL7402 cell lines were established.
发酵过程中维持低葡萄糖水平可以限制细胞的生长速率，提高质粒稳定性和促进青霉素G酰化酶的合成。
Methods:The influence of ammonium sulfate and glucose on plasmid stability and penicillin G acylase activity were conducted in flask shakes and a 5L fermentor.
结论：成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒，并且该基因在NIH3T3细胞中获得了稳定表达。
CONCLUSION: a recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed, and can be expressed stably in the NIH3T3 cells.
该质粒经过稳定筛选后稳定表达于细胞内，促进人肝细胞的生长，抑制其凋亡，但对非肝脏来源的对照组细胞没有明显影响。
PEG10 gene accelerated the growth of L02 cells and inhibited their apoptosis but it had no conspicuous effect on the non-liver derived cells.
该质粒经过稳定筛选后稳定表达于细胞内，促进人肝细胞的生长，抑制其凋亡，但对非肝脏来源的对照组细胞没有明显影响。
PEG10 gene accelerated the growth of L02 cells and inhibited their apoptosis but it had no conspicuous effect on the non-liver derived cells.
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