结果：建立了新基因HBRP稳定表达的基因工程细胞系。
Results: the stable expression cell system of the novel gene HBRP was constructed.
外源基因在大肠杆菌染色体上的稳定表达并不影响细菌的生长繁殖。
The stable expression of exogenous gene in E. coli chromosome had no effect on the bacterial growth and propagation.
目的构建人神经病靶标酯酶（NTE）双链RNA的稳定表达载体。
Objective To construct the RNA interference expression vector for expression of human neuropathy target esterase (NTE) gene in mammalian cells.
GUS活性检测和胭脂碱分析表明，外源基因在转化细胞内得到稳定表达。
Enzyme activity of GUS and NOS assay demonstrated that foreign genes expressed in the transformed plants and calli.
结果：逆转录病毒载体能有效地将外源性基因导入血管内皮细胞并稳定表达。
Results: The CGRP gene could be effectively transferred into HUVEC and stably expressed.
目的构建人G250真核表达载体，建立稳定表达人G250的小鼠黑色素瘤细胞系。
Objective To construct the human G250 eukaryotic expression vector and establish the stable B16 cell line expressing human G250 in mice.
结果：经荧光显微镜、流式细胞仪及免疫印迹法检测，证实AIRE基因得到了稳定表达。
Results: Stable expression of AIRE was confirmed by fluorescence microscope, FACS and western blotting analysis.
结论：应及时用MTX对细胞株进行再加压筛选以及保存，以保持细胞稳定表达抗体的能力；
Conclusion: MTX selection should be timely used to overcome the instability of the expression of anti HBsAg in CHO HB2 cells.
过氧化氢酶(CAT)只出现一条酶带，其稳定表达，对维持细胞代谢环境的稳定有重要作用。
Catalase (CAT) showed only one band, it's stable expression is important for maintaining the stabilization of cell metabolism circumstance.
结论：成功地建立了人乙酰肝素酶稳定表达细胞株，为其抑制剂的筛选及其功能的研究奠定了基础。
Conclusion: Heparanase stable expression cell lines have been successfully established, which could be of great benefits to the screening and function study of its inhibitors.
目的：建立骨唾液蛋白的稳定表达乳腺癌细胞系，观察骨唾液蛋白在乳腺癌骨转移的整个过程中的作用。
OBJECTIVE: To establish breast cancer cell strains of BSP with stable expression and observe the effect of BSP in the whole process of breast cancer bone metastasis.
“她问道：”大多数ER不稳定表达的病例，或者获得或丢失，在HER 2阳性原发肿瘤病人也发生吗？
Are most of the cases with unstable expression of er, either gains or losses, occurring in patients with HER2-positive primary tumors?
结论建立稳定表达人MUC1T739小鼠膀胱癌细胞株和t739荷瘤小鼠模型，可用于肿瘤疫苗研究。
Conclusion T739 mouse bladder cancer cell lines and tumor-bear model expressing human MUC1 were successfully established for the development of tumor vaccines targeting MUC1.
本研究旨在构建稳定表达T7RNA聚合酶的猪睾丸细胞系(ST细胞系)，用于猪瘟病毒等的反向遗传操作。
This study was aimed to establish a ST-based cell line stably expressing the T7 RNA polymerase, which can be used in the reverse genetic manipulation of CSFV and other swine RNA viruses.
目的：建立稳定表达nt3的NIH3T3细胞株，通过体外共培养实验观察其对耳蜗螺旋神经节细胞生长的影响。
AIM: to establish a NIH3T3 cell line which expresses human NT3 and to observe the growth of cochlear ganglion cells by co culturing.
结论构建了含人pd - 1基因重组逆转录病毒的载体，筛选出稳定表达人PD - 1分子的L929细胞株。
Conclusion HumanPD-1 gene has been cloned, recombinant retrovirus vector containing PD-1 gene constructed and L929 transfection cell line expressing PD-1 molecules selected.
结果单、双自杀基因均在GLC- 82细胞中稳定表达，双基因转染组对细胞增殖的杀伤及旁杀伤效应高于单基因组。
Results Double and single suicide gene transfer were both stably expressed in GLC-82 cells. The cytotoxic effects of co-expressed TK-CD genes were superior than that of the single gene.
该质粒经过稳定筛选后稳定表达于细胞内，促进人肝细胞的生长，抑制其凋亡，但对非肝脏来源的对照组细胞没有明显影响。
PEG10 gene accelerated the growth of L02 cells and inhibited their apoptosis but it had no conspicuous effect on the non-liver derived cells.
结论：成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒，并且该基因在NIH3T3细胞中获得了稳定表达。
CONCLUSION: a recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed, and can be expressed stably in the NIH3T3 cells.
为满足转目的基因的需求，探索一种能够将外源基因转入银杏细胞，并得以稳定表达和遗传的转基因方法以及选择合适的载体。
To meet the demands of gene transfer of an objective gene, a suitable vector should be screened to carry the extraneous objective gene into the callus cell of Ginkgo biloba.
这些结果证明供体燕麦上可能携带抗小麦条锈菌的新基因，并已通过花粉管通道进入小麦，整合到小麦的染色体组中，稳定表达。
The result also indicated that the new genes resistant to yellow rust may be carried on oat DNA, which have been integrated into the genome of wheat and expressed steadily.
目的：建立稳定表达尿素通道蛋白a2 (UT - A 2)的FRT细胞系，为寻找UT - A 2抑制剂提供细胞模型。
AIM: to obtain an FRT cell line that can stably express urea transporter A2 (UT-A2) and provide a cell model for screening UT-A2 inhibitors.
目的：建立稳定表达EGFP标记的葡萄糖转运蛋白4的CHO细胞系，为研究GLUT4在CHO细胞中的转运调节机制奠定基础。
Objective:To establish a stable CHO cell line expressing GLUT4-EGFP, and to lay a foundation for investigating the translocation mechanisms of GLUT4 in CHO cells.
但对于一个特定的目标蛋白，选用何种受体植物更有利于外源蛋白的高效稳定表达和经济快捷的提纯，仍是需要首要考虑和解决的问题。
But for a special goal protein, it is the key question to choice the plant that can be in favor of foreign proteins' expressing and purifying.
不同材料间具有稳定表达的组成型RRMRNA结合蛋白基因，同时具有丰富的差异表达的具调控功能的RRM RNA结合蛋白基因。
The primer was designed based on consensus sequence from RRM RNA binding protein gene family. The results showed that there were stable expressed fragments among the different mRNA samples.
表明第7染色体上位于标记R1357~R1245之间的种子休眠性QTL位点是一个在年度之间稳定表达的控制种子休眠性的基因位点。
The QTL for seed dormancy on chromosome 7 between the marker R1357 and R1245 was a stable expressional locus associated with seed dormancy in different years.
结论：稳定表达人ox40l的乳腺癌转基因细胞在体外能有效地活化t细胞，介导其增殖、分泌细胞因子及抑制T细胞活化诱导的细胞死亡。
CONCLUSION: OX40L-MDA-MB-435 cells could activate t cells in vitro, promote t cell proliferation and cytokine secretion, and suppress t cell activation-induced cell death.
目的为建立能稳定表达人葡糖醛酸转移酶UDPGT1A9蛋白的CHL UDPGT1A9转基因细胞系，并鉴定其对药物的葡糖醛酸缀合活性。
AIM To establish a cell line CHL UDP glucuronosyltransferase gene (UDPGT1A9) which will stably express human UDPGT1A9 protein and determine the activity of expressed UDPGT1A9 in drug glucuronidation.
当大脑试图逼迫不稳定的手去完成任务时，两者之间的紧张关系导致的结果是更加精简、心理上更加难懂的表达。
As the brain attempts to force the unsteady hand to do its bidding, the tension between the two results in a more compressed, psychologically denser expression.
尽管当地居民对这次权力转移的合法性表达了不同的看法，但大多数人希望国家和睦与稳定。
Residents expressed different opinions on the legality of the transition, but most expressed hope for peace and stability.
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