方法构建携带CMV - DNA的标准质粒，利用常规pcr技术及FQ - P CR技术分别对不同稀释度的标准质粒进行检测。
Methods to construct the standard plasmid carrying HCMV-DNA; PCR and FQ-PCR techniques were used to detect the plasmid with different dilution.
方法采用方阵滴定法，比较4种封闭液、不同稀释度的捕获抗体、检测抗体和辣根过氧化酶标记链菌素对检测结果的影响，确定ELISA法的最佳实验条件。
Method 4 kinds of block buffer, the different concentration of anti-MIF and biotin-anti-MIF, the different dilution of streptavidin-HRP are used for titration of ELISA test.
将甲型副伤寒沙门菌标准菌株按10- 1 ～ 10 -9稀释后扩增比较PCR的检测灵敏度。
The sensitivity of PCR assay was analyzed with 10 -1 ~10 -9 diluted.
将甲型副伤寒沙门菌标准菌株按10- 1 ～ 10 -9稀释后扩增比较PCR的检测灵敏度。
The sensitivity of PCR assay was analyzed with 10 -1 ~10 -9 diluted.
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