方法：应用DE52离子层析法。
本文研兖了木糖醇、阿糖醇、山梨糖醇在离子交换树脂层析柱上的分离。
The paper discussed the separation of Xylitol, Arabitol and Sorbitol by Ion Exchange Chromatography.
单峰胰岛素经deae琼脂糖快流速离子交换层析后用醋酸锌结晶，得到结晶单组分胰岛素。
Single peak insulin was purified by using DEAE-Sepharose fast flow ion exchange chromatography and crystallizing with zinc component.
菌体经过超声破碎后反复洗涤包涵体，融合蛋白纯度达到70%，进一步用离子交换层析和凝胶过滤层析纯化使其纯度达95%以上。
The purity of fusion protein was about 70% after ultrasonic decomposition and washing repeatedly, and reached above 95 % after purification by ion exchange and gel-filtration chromatography.
利用DEAE离子交换和atp亲和层析法从热休克的大鼠肝脏中分离纯化了热休克蛋白70 (HSP70)。
Heat shock protein 70 (HSP70) was purified from rat liver with DEAE ion exchange chromatography and ATP-affinity chromatography.
研究了离子沉淀法和大孔吸附树脂层析法结合制备茶多酚的工艺。
Combining the ion precipitation with column chromatography to prepare tea polyphenol was studied.
用高速离心、分段盐析、亲和层析、超滤和阴离子交换层析法，从牛胎盘中分离纯化出一种肝细胞生长因子。
A kind of hepatocyte growth factor was purified from cow placenta through high speed centrifugation, salting out, affinity chromatography, ultrafiltration and anion exchange chromatography.
采用硫酸铵沉淀法和阴离子交换层析法从鸡蛋清内提取高纯度卵铁传递蛋白。
High purity ovotransferrin was extracted from albumen by precipitation with ammonium sulfate and ion exchange chromatography in the study.
研究了利用批量离子交换层析与凝胶过滤层析相结合的方法纯化类人胶原蛋白i的最佳条件。
The method of batch ion exchange chromatography combined with gel filtration chromatography to purify Human-like Collagen Biopolymer I was investigated.
采用亲和层析和离子交换层析分离纯化，以细胞增殖实验测定表达蛋白的生物活性。
Affinity chromatography and ion exchange chromatography were applied in isolation and purification, and the bioactivity of expression protein was determined in cell proliferation test.
实验结果表明，等电点沉淀、凝胶柱层析法和阴离子交换法适合于分离水牛乳中主要过敏原成分。
The results showed that the methods of isoelectric precipitation, gel-filtration chromatography and anion-exchange chromatography were suitable for purifying the major allergens in buffalo milk.
目的：选择多核苷酸磷酸化酶快速离子交换层析的最佳条件。
OBJECTIVE:Selection of optimal chromatographic conditions for purification of polynucleotide phosphorylase in fast ion-exchange chromatography.
根据蛋白质所带电荷的不同而进行分离的一种柱层析技术。层析柱用离子装填。
A type of column chromatography in which proteins are separated according to charge. Column is loaded with ions.
凝血酶经CM离子交换液相层析柱进一步纯化。
Thrombin was further purified with CM ion-exchange liquid chromatographic column.
用离子交换柱层析法分离，用反相高效液相色谱法进一步纯化，经聚丙烯酰胺凝胶电泳分析确定相对分子质量。
Methods The peptide was separated by ion exchange column chromatography and further purified by reverse phase HPLC, and the molecular weight was determined by tricine gel electro phoresis.
以离子交换和凝胶过滤层析对梧桐花粉变应原进行分离和纯化。
Two major allergens PT 13 and PT 53 in platanus hispanica pollen were isolated and purified by ion exchange chromatography and gel filtration chromatography.
以含有多个氮原子的组氨酸对强碱型阴离子树脂进行修饰，获得一种新型的离子交换剂。用该离子交换剂对透明质酸粗品进行离子交换层析，使透明质酸与蛋白质、核酸达到有效的分离。
A novel ion exchanger prepared from a strong anion resin modified with histidine was used for purifying crude hyaluronic acid to achieve a successful separation from protein and nucleic acids.
采用DEAE—纤维素离子交换柱层析法对红枣多糖进行组分分析，可得到五种多糖组分。
Using DEAE-Cellulose to separate the polysaccharides from jujube Mill, five content has been acquired.
方法：采用离子交换柱层析和凝胶柱层析纯化得到RRTP；
Method: RRTP-1 was obtained by ion exchange column chromatography and gel chromatography.
通过固定化金属亲和层析、阴离子交换、疏水层析融合蛋白得到了纯化，其纯度可达91%。
By immobilized metal affinity chromatography, ion-exchange chromatography and hydrophobic interaction chromatography, the recombinant Gln49-PLA2 was purified with 91% purity.
融合蛋白基因在巴斯德毕赤酵母中进行表达后通过阳离子交换层析、反向层析和凝胶过滤对表达产物进行了分离纯化。
HSA-AX15(R13K ) fusion protein was purified to homogeneity by cation exchange chromatography, re verse phase chromatography and gel filtration after expressed in pichia pastor is.
抗菌肽的分离应用离子交换层析法将抗菌肽初步分离成两部分即阴离子组分和阳离子组分。
Isolation of the crude extract the crude extract were isolated into two parts, the negative-charged and the positive-charged, by means of Ion-exchange chromatography.
采用镍离子螯合层析法纯化变性的包涵体样品，纯度达90%以上。
The denatured inclusion bodies were purified with Ni-NTA chelate agarose and its purity can reach 90%.
通过脱蛋白、阴离子交换层析、真空干燥步骤分离纯化水溶性多糖与胞外多糖；
The process for preparing extracellular polyose includes centrifugal filter, vacuum concentrating, dialysis desalting and vacuum drying.
进一步提供了可选择的额外的纯化步骤，包括溶胞产物过滤、阴离子交换层析、三联体亲和层析和疏水性相互作用层析。
It further provides for optional additional purification steps, including lysate filtration, anion exchange chromatography, triplex affinity chromatography, and hydrophobic interaction chromatography.
复性结束后用阳离子交换层析纯化。
The refolded endostatin was then purified by cation-exchange chromatography.
表达上清经阳离子交换层析分离纯化为单一组份，SDS - PAGE显示相对分子质量为6 500。
The expression supernatant was purified with cation exchange chromatography to single peak and SDS-PAGE indicated that the relative molecular mass was 6 500.
表达上清经阳离子交换层析分离纯化为单一组份，SDS - PAGE显示相对分子质量为6 500。
The expression supernatant was purified with cation exchange chromatography to single peak and SDS-PAGE indicated that the relative molecular mass was 6 500.
应用推荐