研究了利用批量离子交换层析与凝胶过滤层析相结合的方法纯化类人胶原蛋白i的最佳条件。
The method of batch ion exchange chromatography combined with gel filtration chromatography to purify Human-like Collagen Biopolymer I was investigated.
利用DEAE离子交换和atp亲和层析法从热休克的大鼠肝脏中分离纯化了热休克蛋白70 (HSP70)。
Heat shock protein 70 (HSP70) was purified from rat liver with DEAE ion exchange chromatography and ATP-affinity chromatography.
采用亲和层析和离子交换层析分离纯化，以细胞增殖实验测定表达蛋白的生物活性。
Affinity chromatography and ion exchange chromatography were applied in isolation and purification, and the bioactivity of expression protein was determined in cell proliferation test.
用离子交换柱层析法分离，用反相高效液相色谱法进一步纯化，经聚丙烯酰胺凝胶电泳分析确定相对分子质量。
Methods The peptide was separated by ion exchange column chromatography and further purified by reverse phase HPLC, and the molecular weight was determined by tricine gel electro phoresis.
制备了弱阴离子交换整体柱，并建立了白介素18在该柱上分离纯化的方法。
A weak anion exchange column was prepared and a separation method of interleukin-18 (IL-18) was developed on the column.
目的研究极性有机溶剂辅助阴、阳离子交换色谱串接组合纯化蛋白质的可行性。
PurposeTo explore the possibility of combination of anion and cation exchange chromatography by using the polarity organic solvents in eluent.
凝血酶经CM离子交换液相层析柱进一步纯化。
Thrombin was further purified with CM ion-exchange liquid chromatographic column.
用高速离心、分段盐析、亲和层析、超滤和阴离子交换层析法，从牛胎盘中分离纯化出一种肝细胞生长因子。
A kind of hepatocyte growth factor was purified from cow placenta through high speed centrifugation, salting out, affinity chromatography, ultrafiltration and anion exchange chromatography.
研究了离子交换树脂分离纯化高麦芽糖浆的新工艺。
A new technology for the separation and cleanse of high maltose syrup with ion exchange resins was proposed and studied.
利用离子交换树脂纯化和分离玉米淀粉糖在5 0年代初得到了应用。
The purification and separating corn starch sugar with ion exchange resin have been applied in the early of 1950s.
菌体经过超声破碎后反复洗涤包涵体，融合蛋白纯度达到70%，进一步用离子交换层析和凝胶过滤层析纯化使其纯度达95%以上。
The purity of fusion protein was about 70% after ultrasonic decomposition and washing repeatedly, and reached above 95 % after purification by ion exchange and gel-filtration chromatography.
目的考察阳离子交换树脂法中不同洗脱剂对苦参总碱的纯化效果，优选最佳纯化工艺。
Objective to select the optimum purifying process of total alkaloids in Sophora flavescens by different eluents on cation exchange resin.
方法采用优球蛋白沉淀、离子交换色谱、(NH4)2so4沉淀和亲和色谱法进行纯化。
Methods a combination of euglobulin precipitation, ion-exchange chromatography, (NH4) 2so4 precipitation and affinity chromatography was used in the process of purification.
以离子交换和凝胶过滤层析对梧桐花粉变应原进行分离和纯化。
Two major allergens PT 13 and PT 53 in platanus hispanica pollen were isolated and purified by ion exchange chromatography and gel filtration chromatography.
采用离子交换分离装置和活性炭吸附分离装置进行果汁纯化、玉米糖浆脱灰、甜菜糖纯化、甜味剂脱色的工厂日益增多。
Juice purification, corn syrup deashing, beat sugar purification and sweetener decolorization are practiced daily in many ion Exchange Separations and Activated Carbon Adsorption installations.
方法：采用离子交换柱层析和凝胶柱层析纯化得到RRTP；
Method: RRTP-1 was obtained by ion exchange column chromatography and gel chromatography.
通过对溶剂、提取次数、碱化条件的选择，用离子交换和重结晶的方法提取纯化氧化苦参碱。
Through selecting extracting solvent, extracting times and alkalinizing method, Oxymatrine is extracted and purified by ion-exchanging and re-crystallization.
目的：探索以阴、阳离子交换色谱串接组合为方式，进行高分辨率分离纯化蛋白质的可行性。
Objective To explore the possibility of achieving the highest resolution by using combination of anion and cation exchange chromatography.
以大孔吸附树脂提取的大豆皂苷粗提物为研究对象，利用阳离子交换树脂进行纯化。
A cation exchange resin was applied to purify the crude soyasaponins obtained by solid phase extraction (SPE) using AB-8, a kind of polystyrene matrix macroporous absorbent resins.
采用二次离子交换法分离天然样品中的硼是一种更有效的从天然样品中分离和纯化硼的分离方法，因而，也进一步提高了硼同位素组成测定的测量精度。
The results showed that boron separation by the two-step ion exchange for isotopic measurement was more effective. It achieved high accuracy of isotopic measurement of boron.
结论用离子交换纤维提取纯化溶解在乙醇中的葛根素的方法是可行的；
Conclusion The method of using ion exchange fiber to extracting and refining puerarin by dissolving alcohol is feasible;
中空纤维离子交换膜广泛应用于蛋白质的分离纯化、重金属离子的去除以及酶催化反应等众多领域。
Hollow fiber ion-exchange membrane is widely used in many areas such as protein separation and purification, removal of heavy metal ion and enzyme immobilization.
融合蛋白基因在巴斯德毕赤酵母中进行表达后通过阳离子交换层析、反向层析和凝胶过滤对表达产物进行了分离纯化。
HSA-AX15(R13K ) fusion protein was purified to homogeneity by cation exchange chromatography, re verse phase chromatography and gel filtration after expressed in pichia pastor is.
通过固定化金属亲和层析、阴离子交换、疏水层析融合蛋白得到了纯化，其纯度可达91%。
By immobilized metal affinity chromatography, ion-exchange chromatography and hydrophobic interaction chromatography, the recombinant Gln49-PLA2 was purified with 91% purity.
进一步提供了可选择的额外的纯化步骤，包括溶胞产物过滤、阴离子交换层析、三联体亲和层析和疏水性相互作用层析。
It further provides for optional additional purification steps, including lysate filtration, anion exchange chromatography, triplex affinity chromatography, and hydrophobic interaction chromatography.
目的：考察阳离子交换树脂不同洗脱剂纯化苦参总生物碱的效果，优选最佳纯化工艺条件。
Objective: to select the optimum purifying process of total alkaloids from Sophora flavescens by means of cation exchange resin.
表达上清经阳离子交换层析分离纯化为单一组份，SDS - PAGE显示相对分子质量为6 500。
The expression supernatant was purified with cation exchange chromatography to single peak and SDS-PAGE indicated that the relative molecular mass was 6 500.
复性结束后用阳离子交换层析纯化。
The refolded endostatin was then purified by cation-exchange chromatography.
通过脱蛋白、阴离子交换层析、真空干燥步骤分离纯化水溶性多糖与胞外多糖；
The process for preparing extracellular polyose includes centrifugal filter, vacuum concentrating, dialysis desalting and vacuum drying.
通过脱蛋白、阴离子交换层析、真空干燥步骤分离纯化水溶性多糖与胞外多糖；
The process for preparing extracellular polyose includes centrifugal filter, vacuum concentrating, dialysis desalting and vacuum drying.
应用推荐