采用富集到的磷酸化肽样品可进而实现磷酸化位点的鉴定。
The enrichment sample of phosphorylated peptide can identify phosphorylation sites.
最后用串联质谱分析结合数据库检索鉴定用来确定磷酸化位点。
Finally, the phosphorylation sites are determined by tandem mass spectrometry analysis and database searching.
激光共聚焦显微镜观察完整的和去除蛋白激酶A磷酸化位点的线粒体融合素2基因在细胞中的定位。
Laser confocal microscopy was used to observe the locations of Mfn2 gene with and without PKA in cells.
例如，我们使用GPS和贝叶斯决策理论算法，分别构建了预测蛋白质磷酸化位点的GPS和PP SP网站。
For example, we construct GPS and PPSP for phosphorylation site prediction, based on GPS and Bayesian Decision Theory algorithms, respectively.
类似这种的脯氨酸导向的磷酸化位点通常是CDKs和MAPKs的靶标，并往往能显著影响蛋白质的构象（4，5）。
Proline-directed phosphorylation sites like this one are often targeted by CDKs and MAPKs and can often dramatically affect protein conformation (4,5).
其激活和转位机制可能与其不同的活化因子或底物和不同位点的氨基酸残基磷酸化有关。
The mechanisms of its activation and translocation may be associated with the difference of the activation factors or its targets, and the amino acid residues of different phosphorylation site.
所有先天感染患儿临床株的UL145编码蛋白具有蛋白激酶（C PKC）磷酸化功能位点和酪蛋白激酶（CK2）磷酸化功能位点。
The UL145 protein of the clinical strains were conserved and comprised phosphorylation motif sites for protein kinase C(PKC) and casein kinase 2(CK2).
此过程的上游激酶和这些位点磷酸化的生物学意义尚不明晰(6)。
The upstream kinase and biological significance of these phosphorylation events have yet to be elucidated (6).
这些地方是已知的ATM/ATR激酶磷酸化的首选作用位点（4，5）。
These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5).
该抗体不与其他位点磷酸化的PP2A亚单位发生交叉反应。
该抗体不与其他位点磷酸化的Chk1蛋白发生交叉反应。
This antibody does not cross-react with Chk1 when phosphorylated at other sites.
该抗体不与其他位点磷酸化的p53 蛋白发生交叉反应。
The antibody does not cross-react with p53 phosphorylated at other sites.
该抗体不与其他位点磷酸化的p 53蛋白发生交叉反应。
It does not cross-react with p53 phosphorylated at other sites.
本抗体不与其它酪氨酸位点被磷酸化的ErbB家族蛋白发生交叉反应。
This antibody does not cross-react with other tyrosine-phosphorylated ErbB family members.
本抗体不与其它酪氨酸位点被磷酸化的ErbB家族蛋白发生交叉反应。
This antibody does not cross-react with other tyrosine-phosphorylated ErbB family members.
应用推荐