为提高碱性磷酸酶（ALP）活性测定的灵敏度，对以4-甲基伞形酮磷酸酯（4-MUP）为底物的荧光法进行了研究。
In order to improve the sensitivity of detection of alkaline phosphatase(ALP)activity, a fluorometric method based on 4-methylumbelliferyl phosphate(4-MUP)as substrate was investigated.
同时测定了培养组织的碱性磷酸酶活性，培养组织与河蚌外套膜具有相近的比活及相对酶活。
The specific and relative activities of alkaline phosphatase were similar for both nature and cultured tissues of mantles in this experiment.
方法：采用经具核梭杆菌LPS刺激的单核细胞培养上清作用于体外培养的人牙髓细胞，通过OD值测定，观察单核细胞培养上清对人牙髓成纤维细胞碱性磷酸酶（ALP）活性的影响。
Methods:The culture supernatant of monocytes stimulated with Fn LPS was applied to human dental pulp cell in vitro, and activity of ALP in human dental pulp fibroblast was monitored by OD test.
方法：采用经具核梭杆菌LPS刺激的单核细胞培养上清作用于体外培养的人牙髓细胞，通过OD值测定，观察单核细胞培养上清对人牙髓成纤维细胞碱性磷酸酶（ALP）活性的影响。
Methods:The culture supernatant of monocytes stimulated with Fn LPS was applied to human dental pulp cell in vitro, and activity of ALP in human dental pulp fibroblast was monitored by OD test.
应用推荐