方法采用碱性磷酸酶抗碱性磷酸酶法(APAAP法)检测48例急性荨麻疹患者和20例健康对照者外周血CD 3 +，CD 4 +，CD 8 +T淋巴细胞。
Methods the peripheral blood t lymphocytes and their subsets in 48 patients with acute urticaria were detected by APAAP and compared with 20 healthy control.
方法：用碱性磷酸酶抗碱性磷酸酶法检测再障患者骨髓中T淋巴细胞表型的变化，并用半固体培养法检测再障患者骨髓及去T细胞后骨髓单个核细胞CFU-GM集落形成情况。
Methods: Phenotype of T lymphocytes in bone marrow and CFU GM yields of bone marrow mononuclear cells with or without T cells were assayed with APAAP and semi solid culture method, respectively.
采用酶动力法测成骨细胞在基质材料上培养2，4，6 天后的碱性磷酸酶（ALP）活性。
Alkaline phosphatase (ALP) activity of osteoblasts was determined by enzyme dynamical method at 2d, 4d and 6d .
为提高碱性磷酸酶（ALP）活性测定的灵敏度，对以4-甲基伞形酮磷酸酯（4-MUP）为底物的荧光法进行了研究。
In order to improve the sensitivity of detection of alkaline phosphatase(ALP)activity, a fluorometric method based on 4-methylumbelliferyl phosphate(4-MUP)as substrate was investigated.
通过扫描电镜、MTT测试法、考马斯亮蓝和碱性磷酸酶活性检测法，观察去抗原异种松质骨材料对细胞生长、增殖及功能表达的影响。
SEM, MTT assay and alkaline phosphatase (ALP) activity were carried out to observe the effect of AEXCB on cell growth, proliferation and functional expression.
骨代谢生化指标测量：全自动生化分析仪测血清钙、磷、碱性磷酸酶、抗酒石酸酸性磷酸酶，放免法测骨钙素；
Measurement of biochemical markers of bone metabolism: Serum Ca, P, ALP and TRAP was measured by automatic biochemistry analyzer, BGPmeasured by radioimmunoassay;
骨代谢生化指标测量：全自动生化分析仪测血清钙、磷、碱性磷酸酶、抗酒石酸酸性磷酸酶，放免法测骨钙素；
Measurement of biochemical markers of bone metabolism: Serum Ca, P, ALP and TRAP was measured by automatic biochemistry analyzer, BGPmeasured by radioimmunoassay;
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