目的探讨中性粒细胞碱性磷酸酶染色的实验条件。
Objective To discuss the experimental conditions of neutrophil alkaline phosphatase staining.
目的建立一种简便快速中性粒细胞碱性磷酸酶染色方法。
Aim To establish a rapid method of neutrophil alkaline phosphatase stain.
诱导培养细胞碱性磷酸酶染色，骨钙素免疫组化染色阳性。
The hMSCs in induced culture showed cytochemical staining for ALP and osteocalcin expression.
采用碱性磷酸酶染色、核型分析、类胚体实验对扩增细胞进行ESC鉴定。
Subsequently we identified the expanding cells by alkaline phosphatase staining, karyotype analysis and the formation of embryoid body which are typical characteristics of ESCs.
结果从兔颅骨分离的细胞碱性磷酸酶染色阳性，在长期培养中能够形成钙化结节。
Results The cells from rabbit calvaria were positive to ALP staining and could form calcium node in vitro.
空白组碱性磷酸酶染色均显示胞浆呈粉红色，细胞仍然保留清晰的成纤维细胞样外观。
ALP staining of the blank group have shown that the cytoplasm was pink, the cells still retain a clear fibroblast-like appearance.
方法：采用碱性磷酸酶染色法，对7例新生儿和婴幼儿脑视觉系统微血管密度进行定量分析。
Methods: The microvascular densities were observed and analyzed in alkaline phosphatase-stained sections of human visual system from 7 neonates, infants and child brains.
结果转染后成骨细胞的基因组中整合有SV40T基因，碱性磷酸酶染色阳性细胞占95％以上；
Results Homologous integration of SV40T gene into RAE - 1 cell genome was positively detected by PCR. More than 95% cells were proved positively by alkaline phosphatase staining.
主要观察指标：形态观察、增值率测定、碱性磷酸酶染色、细胞基质钙含量测定、茜素红染色显示矿化结节数。
MAIN OUTCOME MEASURES: Morphological observation, determination of proliferation ratio, ALP stain, determination of matrix calcium accumulation and number of mineralized nodes with alizarin red stain.
碱性磷酸酶染色：细胞诱导培养第3代后可见胞质内有大量灰黑色颗粒或块状沉淀，呈现碱性磷酸酶染色阳性。
Alkaline phosphatase staining: After the 3rd generation culture, there was a large amount of gray-black granule or block deposit, which showed positive alkaline phosphatase staining.
用磷酸麝香草酚酞中释放麝香草酚酞方法检测碱性磷酸酶ALP活性用硫酸茜红素染色法检测基质矿化。
Alkaline phosphatase (ALP) activity was evaluated by thymolphthalein release from thymolphthalein monophosphate and matrix mineralization by staining with alizarin red S.
方法：(1)建立与改良小鼠成骨细胞的原代培养方法，并通过碱性磷酸酶和钙结节染色方法进行细胞来源鉴定。
Methods: (1) We built and improved the method of osteoblasts primary culture in vitro in mice, and identified the origination of osteoblasts by AKP and calcium nodes dyeing.
方法：应用碱性磷酸酶血管染色法，对脊髓内部血管，用体视学方法作计量和观察。
Methods: Blood vessels of spinal cord were stained with alkaline phosphatase and then observed with stereology method.
方法用碱性磷酸酶-抗碱性磷酸酶（APAAP）免疫酶标和DNA末端原位标记（ISEL）双重染色方法检测38例急性白血病诱导化疗前后骨髓涂片中细胞凋亡程度及细胞类型，初治组17例与2 1例经治病例对照。
Methods A double-stained way of APAAP and in situ DNA end labeling (ISEL) were used to detect apoptosis characteristics in 38 cases of acute leukemia, including 17 untreated and 21 treated patients.
方法用碱性磷酸酶-抗碱性磷酸酶（APAAP）免疫酶标和DNA末端原位标记（ISEL）双重染色方法检测38例急性白血病诱导化疗前后骨髓涂片中细胞凋亡程度及细胞类型，初治组17例与2 1例经治病例对照。
Methods A double-stained way of APAAP and in situ DNA end labeling (ISEL) were used to detect apoptosis characteristics in 38 cases of acute leukemia, including 17 untreated and 21 treated patients.
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